ZNF513 (NM_144631) Human Tagged ORF Clone

CAT#: RG208630

ZNF513 (tGFP-tagged) - Human zinc finger protein 513 (ZNF513), transcript variant 1

Product Images

Circular map for RG208630

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF513
• Synonyms: HMFT0656; RP58; Zfp513
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208630 representing NM_144631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGAAGGAAGCAAAGCCACCCGCAGCCCGTGAAATGCGAGGGGGTCAAAGTGGATACTGAAGACT
  CCCTCGACGAAGGACCCGGGGCCCTGGTATTGGAGAGTGATTTGCTACTAGGCCAGGATCTGGAGTTTGA
  GGAGGAAGAGGAAGAGGAGGAAGGCGACGGCAACAGTGACCAGCTCATGGGCTTCGAGAGAGACTCGGAA
  GGAGACTCTCTGGGGGCCAGGCCTGGGCTTCCCTATGGGCTGAGCGACGATGAGTCTGGGGGCGGCCGGG
  CACTAAGTGCGGAGAGTGAAGTTGAGGAGCCAGCCAGGGGTCCAGGGGAGGCCAGGGGTGAGAGGCCAGG
  CCCAGCCTGCCAGCTGTGTGGGGGGCCGACAGGTGAGGGGCCGTGTTGTGGGGCAGGAGGGCCGGGTGGG
  GGGCCCCTGCTGCCCCCACGGCTACTGTACTCATGCCGCCTCTGCACCTTCGTGTCCCACTACTCGAGCC
  ACCTGAAGCGGCACATGCAGACACACAGCGGAGAGAAGCCGTTCCGCTGTGGCCGCTGCCCCTACGCCTC
  AGCCCAGCTCGTCAACCTGACACGACATACCCGCACCCACACTGGCGAGAAGCCCTACCGCTGTCCCCAC
  TGCCCCTTTGCCTGCAGCAGCCTGGGCAACCTGAGGCGGCATCAGCGTACCCACGCAGGGCCCCCCACTC
  CTCCCTGCCCGACCTGTGGCTTCCGCTGCTGTACTCCACGACCAGCCCGGCCTCCCAGTCCCACAGAGCA
  GGAGGGGGCGGTGCCCCGGCGACCTGAAGATGCTCTGCTCCTTCCAGATTTGAGCCTCCATGTGCCACCA
  GGTGGTGCCAGTTTCCTGCCAGACTGTGGGCAGCTGCGGGGTGAAGGGGAGGGCCTCTGCGGGACTGGAT
  CAGAACCACTGCCAGAGCTGCTATTCCCTTGGACCTGCCGGGGCTGTGGACAAGAGCTGGAGGAGGGTGA
  GGGTAGTCGGCTGGGAGCTGCCATGTGTGGGCGCTGCATGCGAGGAGAGGCTGGAGGGGGTGCCAGTGGG
  GGGCCCCAGGGCCCCAGTGACAAAGGCTTTGCCTGTAGCCTCTGCCCCTTTGCCACTCACTATCCCAACC
  ACCTGGCCCGGCACATGAAGACACACAGTGGTGAGAAGCCCTTCCGCTGCGCCCGCTGTCCTTATGCCTC
  TGCTCATCTGGATAACCTGAAACGGCACCAGCGCGTCCATACAGGAGAGAAGCCCTACAAGTGCCCCCTC
  TGCCCTTATGCCTGTGGCAATCTGGCCAACCTCAAGCGTCATGGTCGCATCCACTCTGGTGACAAACCTT
  TTCGGTGTAGCCTTTGCAACTACAGCTGCAACCAGAGCATGAACCTCAAACGTCACATGCTGCGGCACAC
  AGGCGAGAAGCCCTTCCGCTGTGCCACCTGCGCCTATACCACGGGCCACTGGGACAACTACAAGCGCCAC
  CAGAAGGTGCATGGCCACGGTGGGGCAGGAGGGCCTGGTCTCTCTGCCTCTGAGGGCTGGGCCCCACCTC
  ATAGCCCACCCTCTGTTTTGAGCTCTCGGGGCCCACCAGCCCTGGGGACTGCTGGCAGCCGGGCTGTCCA
  CACAGACTCATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208630 representing NM_144631
  Red=Cloning site Green=Tags(s)
  
  MPRRKQSHPQPVKCEGVKVDTEDSLDEGPGALVLESDLLLGQDLEFEEEEEEEEGDGNSDQLMGFERDSE
  GDSLGARPGLPYGLSDDESGGGRALSAESEVEEPARGPGEARGERPGPACQLCGGPTGEGPCCGAGGPGG
  GPLLPPRLLYSCRLCTFVSHYSSHLKRHMQTHSGEKPFRCGRCPYASAQLVNLTRHTRTHTGEKPYRCPH
  CPFACSSLGNLRRHQRTHAGPPTPPCPTCGFRCCTPRPARPPSPTEQEGAVPRRPEDALLLPDLSLHVPP
  GGASFLPDCGQLRGEGEGLCGTGSEPLPELLFPWTCRGCGQELEEGEGSRLGAAMCGRCMRGEAGGGASG
  GPQGPSDKGFACSLCPFATHYPNHLARHMKTHSGEKPFRCARCPYASAHLDNLKRHQRVHTGEKPYKCPL
  CPYACGNLANLKRHGRIHSGDKPFRCSLCNYSCNQSMNLKRHMLRHTGEKPFRCATCAYTTGHWDNYKRH
  QKVHGHGGAGGPGLSASEGWAPPHSPPSVLSSRGPPALGTAGSRAVHTDSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144631
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144631.6
• RefSeq Size: 2168 bp
• RefSeq ORF: 1626 bp
• Locus ID: 130557
• UniProt ID: Q8N8E2
• Cytogenetics: 2p23.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a possible transcriptional regulator involved in retinal development. Defects in this gene can be a cause of autosomal-recessive retinitis pigmentosa. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]