MYBBP1A (NM_014520) Human Tagged ORF Clone

CAT#: RG208514

MYBBP1A (tGFP-tagged) - Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2

Product Images

Circular map for RG208514

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MYBBP1A
• Synonyms: P160; PAP2; Pol5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208514 representing NM_014520
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCCGGGATCCCGCCCAGCCGATGTCGCCTGGAGAAGCGACGCAGAGTGGCGCCCGGCCTGCCG
  ACCGCTATGGCCTATTGAAGCACAGTCGCGAGTTCTTGGACTTCTTCTGGGACATTGCGAAGCCTGAGCA
  GGAGACGCGACTTGCGGCCACGGAGAAGCTGCTGGAGTATCTGCGTGGCAGGCCGAAGGGGTCCGAGATG
  AAATATGCCCTGAAGCGTCTAATCACGGGACTCGGGGTCGGGCGAGAAACAGCCCGGCCCTGCTACAGTT
  TGGCCCTGGCACAGCTGTTACAGTCTTTTGAAGACCTCCCCTTGTGCAGCATCCTGCAGCAGATACAAGA
  AAAATATGACCTGCATCAGGTGAAGAAGGCAATGCTGAGACCTGCTCTCTTTGCAAACCTGTTTGGAGTG
  CTCGCCCTCTTTCAGTCAGGTCGGCTGGTGAAGGACCAGGAGGCACTGATGAAGTCGGTGAAGCTGCTGC
  AGGCCCTGGCCCAGTACCAAAACCACTTGCAGGAGCAGCCCCGGAAGGCCCTGGTGGACATCCTCTCCGA
  GGTCTCGAAGGCCACATTGCAGGAGATCCTGCCGGAGGTCCTCAAAGCCGACTTGAATATAATACTCAGC
  TCCCCTGAACAGCTAGAGCTCTTCCTCCTGGCCCAGCAGAAGGTGCCCTCCAAGCTCAAGAAGCTGGTGG
  GATCCGTGAACCTATTCTCAGATGAGAATGTCCCCAGGCTGGTGAATGTGCTGAAGATGGCCGCCTCCTC
  TGTGAAGAAGGACCGCAAGCTGCCCGCCATTGCTCTGGACCTGCTCCGCCTGGCGCTCAAGGAAGACAAG
  TTCCCACGGTTCTGGAAGGAGGTGGTGGAACAAGGGCTGCTGAAGATGCAGTTCTGGCCAGCCAGCTACC
  TGTGTTTCCGCCTGCTGGGCGCGGCCCTGCCCCTGCTGACCAAGGAGCAGCTGCACCTGGTGATGCAGGG
  AGACGTGATCCGCCATTACGGGGAGCACGTGTGCACTGCTAAGCTCCCAAAGCAGTTCAAGTTTGCCCCA
  GAGATGGACGATTACGTGGGCACCTTCCTAGAGGGGTGCCAGGATGACCCTGAGCGGCAGCTGGCCGTGC
  TAGTGGCCTTCTCATCTGTCACCAACCAAGGCCTCCCTGTCACGCCTACTTTCTGGCGGGTCGTGCGGTT
  CCTGAGCCCTCCGGCCCTGCAGGGCTATGTGGCCTGGCTGCGGGCCATGTTTCTCCAGCCAGACCTGGAC
  TCCTTGGTTGACTTCAGCACCAACAACCAGAAGAAAGCCCAGGATTCATCGCTCCACATGCCTGAGCGAG
  CTGTGTTCCGGCTGAGGAAATGGATCATCTTTCGATTGGTGAGCATTGTGGACAGCCTGCACCTGGAGAT
  GGAGGAGGCCTTGACTGAGCAGGTGGCCAGGTTTTGTTTGTTCCACTCGTTCTTTGTCACAAAGAAGCCC
  ACATCCCAGATCCCTGAGACAAAGCACCCGTTCTCCTTCCCTTTGGAAAACCAGGCCCGAGAGGCTGTCA
  GCAGTGCCTTCTTCAGTCTGTTGCAGACCCTCAGCACGCAGTTCAAGCAGGCACCGGGCCAGACCCAGGG
  TGGGCAGCCCTGGACCTACCACCTGGTGCAGTTCGCAGACCTCCTGTTGAATCACAGCCACAACGTGACC
  ACCGTGACACCCTTCACTGCGCAGCAGCGCCAGGCCTGGGACCGGATGCTGCAGACTCTGAAGGAGCTGG
  AGGCCCACTCCGCAGAGGCCAGGGCTGCTGCCTTCCAGCACCTTCTGCTCCTCGTGGGCATCCACCTCCT
  CAAGTCCCCTGCAGAGAGCTGTGACCTGCTGGGTGACATCCAGACCTGCATCAGGAAAAGTCTGGGAGAG
  AAGCCCCGCCGGAGCCGCACCAAGACCATCGACCCCCAGGAACCCCCGTGGGTAGAGGTGCTGGTGGAGA
  TCTTGCTGGCCCTGTTGGCCCAGCCCAGCCACCTCATGCGCCAGGTGGCCCGGAGCGTGTTTGGCCACAT
  CTGCTCCCACCTGACCCCGCGTGCCCTGCAGCTAATTCTGGATGTGCTGAACCCCGAGACCAGTGAGGAT
  GAGAATGACCGTGTGGTGGTGACGGACGATTCTGATGAGCGGCGGCTGAAGGGTGCAGAGGACAAGAGCG
  AGGAAGGTGAGGACAACAGAAGCTCAGAGAGTGAAGAGGAGAGCGAGGGGGAGGAGAGCGAGGAGGAGGA
  GCGCGACGGGGACGTGGATCAGGGCTTCCGGGAACAGCTGATGACCGTGCTGCAGGCTGGGAAGGCGCTG
  GGTGGAGAGGACAGTGAGAACGAGGAGGAGCTGGGGGATGAGGCCATGATGGCCCTGGACCAGAGCCTCG
  CCAGCCTCTTTGCCGAGCAGAAGCTGCGTATCCAGGCCCGGCGAGACGAGAAGAACAAGCTGCAGAAGGA
  GAAGGCTCTGCGGCGCGACTTCCAGATCCGGGTGCTGGACCTGGTGGAGGTGCTAGTGACCAAGCAGCCC
  GAGAATGCCCTGGTCCTGGAGCTGCTGGAGCCGCTGCTGAGCATCATCCGGCGCAGCCTGCGCAGCAGCA
  GCTCCAAACAGGAGCAGGACCTTCTGCACAAGACGGCGCGCATCTTCACGCACCACCTGTGCCGTGCCCG
  GCGCTACTGCCACGACTTGGGTGAGCGCGCAGGGGCCCTGCACGCCCAGGTGGAGCGGTTGGTGCAGCAG
  GCTGGCCGCCAGCCCGACTCCCCCACCGCCCTCTACCACTTCAACGCCTCTCTCTACCTGCTCCGGGTCT
  TGAAGGGCAACACTGCTGAGGGCTGCGTGCATGAGACACAGGAGAAGCAGAAAGCTGGCACTGACCCCAG
  CCACATGCCCACGGGCCCGCAGGCTGCCAGCTGCTTGGACTTGAACCTGGTGACCCGGGTGTACTCGACA
  GCACTGAGCTCCTTCCTGACCAAGCGCAACAGCCCCCTCACAGTTCCCATGTTCCTCAGCCTCTTCTCCC
  GGCACCCGGTGCTCTGTCAGAGCCTGCTCCCCATCCTGGTCCAGCATATCACGGGCCCGGTGCGGCCCCG
  TCGTCAGGCCTGCCTGCTGCTCCAGAAGACCCTGTCCATGCGGGAGGTGAGGTCGTGCTTTGAGGACCCC
  GAGTGGAAGCAGCTGATGGGCCAGGTCCTAGCAAAGGTCACCGAGAACTTGCGCGTGCTGGGGGAGGCGC
  AGACCAAGGCGCAGCATCAGCAGGCACTGTCCTCCCTGGAGCTGCTCAACGTTCTCTTCAGGACCTGCAA
  ACATGAGAAGCTGACCTTGGACCTGACGGTGCTCCTGGGTGTGCTGCAGGGGCAACAGCAGAGCCTACAG
  CAGGGGGCACACTCCACCGGCTCCAGCCGCCTGCACGACCTCTACTGGCAGGCCATGAAAACCCTGGGAG
  TCCAGCGCCCCAAGTTGGAGAAGAAGGATGCCAAGGAGATCCCCAGTGCCACCCAGAGCCCCATCAGTAA
  GAAGCGGAAGAAAAAGGGATTCTTGCCAGAGACGAAGAAGCGCAAGAAACGCAAGTCAGAGGATGGCACG
  CCAGCGGAGGATGGCACACCTGCAGCCACCGGCGGGAGCCAGCCCCCCAGCATGGGCAGGAAGAAGAGGA
  ACAGGACAAAGGCTAAGGTCCCAGCCCAGGCAAACGGGACGCCAACCACCAAGAGTCCAGCCCCTGGCGC
  CCCCACCCGGAGCCCCAGCACCCCTGCCAAATCCCCAAAACTGCAGAAGAAAAACCAGAAGCCGTCCCAG
  GTGAATGGAGCTCCCGGGTCCCCCACGGAACCTGCAGGCCAAAAGCAGCATCAGAAGGCTCTTCCCAAAA
  AGGGGGTCTTGGGCAAATCACCACTGTCCGCGCTGGCACGGAAAAAGGCAAGGCTGTCTTTGGTCATCAG
  GAGTCCCAGCCTGCTTCAGAGTGGGGCCAAGAAGAAAGCACAGGTGAGGAAGGCAGGGAAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208514 representing NM_014520
  Red=Cloning site Green=Tags(s)
  
  MESRDPAQPMSPGEATQSGARPADRYGLLKHSREFLDFFWDIAKPEQETRLAATEKLLEYLRGRPKGSEM
  KYALKRLITGLGVGRETARPCYSLALAQLLQSFEDLPLCSILQQIQEKYDLHQVKKAMLRPALFANLFGV
  LALFQSGRLVKDQEALMKSVKLLQALAQYQNHLQEQPRKALVDILSEVSKATLQEILPEVLKADLNIILS
  SPEQLELFLLAQQKVPSKLKKLVGSVNLFSDENVPRLVNVLKMAASSVKKDRKLPAIALDLLRLALKEDK
  FPRFWKEVVEQGLLKMQFWPASYLCFRLLGAALPLLTKEQLHLVMQGDVIRHYGEHVCTAKLPKQFKFAP
  EMDDYVGTFLEGCQDDPERQLAVLVAFSSVTNQGLPVTPTFWRVVRFLSPPALQGYVAWLRAMFLQPDLD
  SLVDFSTNNQKKAQDSSLHMPERAVFRLRKWIIFRLVSIVDSLHLEMEEALTEQVARFCLFHSFFVTKKP
  TSQIPETKHPFSFPLENQAREAVSSAFFSLLQTLSTQFKQAPGQTQGGQPWTYHLVQFADLLLNHSHNVT
  TVTPFTAQQRQAWDRMLQTLKELEAHSAEARAAAFQHLLLLVGIHLLKSPAESCDLLGDIQTCIRKSLGE
  KPRRSRTKTIDPQEPPWVEVLVEILLALLAQPSHLMRQVARSVFGHICSHLTPRALQLILDVLNPETSED
  ENDRVVVTDDSDERRLKGAEDKSEEGEDNRSSESEEESEGEESEEEERDGDVDQGFREQLMTVLQAGKAL
  GGEDSENEEELGDEAMMALDQSLASLFAEQKLRIQARRDEKNKLQKEKALRRDFQIRVLDLVEVLVTKQP
  ENALVLELLEPLLSIIRRSLRSSSSKQEQDLLHKTARIFTHHLCRARRYCHDLGERAGALHAQVERLVQQ
  AGRQPDSPTALYHFNASLYLLRVLKGNTAEGCVHETQEKQKAGTDPSHMPTGPQAASCLDLNLVTRVYST
  ALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRRQACLLLQKTLSMREVRSCFEDP
  EWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQ
  QGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGT
  PAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQ
  VNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014520
• ORF Size: 3984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014520.1, NP_055335.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 3987 bp
• Locus ID: 10514
• UniProt ID: Q9BQG0
• Cytogenetics: 17p13.2
• Domains: DNA_pol_V
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]