EFHA2 (MICU3) (NM_181723) Human Tagged ORF Clone

CAT#: RG208462

EFHA2 (tGFP-tagged) - Human EF-hand domain family, member A2 (EFHA2)

Product Images

Circular map for RG208462

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EFHA2
• Synonyms: EFHA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208462 representing NM_181723
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCTGCGAAGGCTCTTGTGGCCGCCACCCCGGGTGTCTCCTCCACTCTGCGCTCACCAGCCCC
  TCCTTGGGCCGTGGGGGCGGCCTGCGGTGACCACCCTGGGCCTTCCTGGCCGGCCCTTCTCCTCCCGAGA
  GGATGAGGAGAGGGCTGTGGCGGAGGCGGCATGGAGGCGGCGGCGGCGCTGGGGGGAGCTGAGCGTGGCG
  GCGGCGGCCGGCGGGGGGCTGGTCGGCCTGGTATGCTACCAGCTGTACGGGGACCCCAGGGCCGGCTCGC
  CGGCGACCGGGCGACCCTCAAAGAGCGCGGCCACGGAGCCCGAGGACCCGCCCCGCGGCCGGGGGATGCT
  GCCCATCCCAGTGGCGGCTGCCAAGGAGACGGTTGCTATTGGCAGAACAGACATTGAAGACTTAGACCTT
  TATGCCACATCTCGGGAGAGGCGATTTCGTTTATTTGCTTCTATAGAATGTGAAGGGCAGTTATTCATGA
  CTCCGTATGATTTTATTTTGGCTGTTACAACAGATGAGCCCAAAGTTGCCAAAACTTGGAAGTCACTTTC
  CAAACAGGAATTAAATCAAATGCTCGCAGAAACACCACCAGTTTGGAAAGGCTCATCGAAGCTATTTCGA
  AATCTTAAAGAAAAAGGTGTGATTTCTTACACAGAATATCTTTTTCTTTTATGTATTTTAACAAAGCCAC
  ATGCAGGGTTCAGAATAGCTTTCAACATGTTTGACACTGATGGCAATGAGATGGTGGATAAAAAAGAGTT
  TTTGGTGCTTCAAGAGATATTCAGGAAAAAAAATGAAAAGAGAGAAATTAAAGGAGATGAAGAAAAGCGT
  GCAATGCTGCGTCTTCAACTTTATGGATACCATTCTCCTACTAATAGTGTACTTAAAACAGATGCTGAGG
  AACTTGTCTCCAGAAGCTATTGGGATACACTGAGACGTAACACAAGCCAAGCACTGTTTTCAGACCTCGC
  AGAGCGTGCTGATGACATCACAAGTTTAGTAACAGATACTACACTTCTTGTACACTTTTTTGGAAAGAAA
  GGAAAAGCTGAGCTCAACTTTGAAGATTTTTATAGATTCATGGATAATCTCCAAACAGAAGTTCTAGAAA
  TAGAATTCCTTTCCTACTCAAATGGAATGAATACCATCAGTGAAGAAGATTTTGCTCATATTCTTTTACG
  ATATACAAATGTGGAAAATACATCAGTATTTTTAGAAAATGTGCGTTACAGTATACCTGAAGAAAAGGGC
  ATCACATTTGATGAATTCAGGTCATTTTTCCAGTTTTTAAACAACCTAGAAGACTTTGCAATAGCCCTGA
  ATATGTATAACTTTGCAAGTCGTTCTATAGGGCAAGATGAATTTAAACGTGCCGTCTATGTAGCTACTGG
  ACTCAAATTTTCACCACATTTAGTGAACACTGTCTTCAAGATTTTTGATGTTGACAAAGATGATCAATTA
  AGTTATAAAGAATTTATTGGAATTATGAAAGACAGACTCCATAGAGGATTCCGGGGTTATAAAACAGTCC
  AGAAGTACCCCACTTTCAAATCCTGCCTGAAGAAAGAACTTCACAGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208462 representing NM_181723
  Red=Cloning site Green=Tags(s)
  
  MAALRRLLWPPPRVSPPLCAHQPLLGPWGRPAVTTLGLPGRPFSSREDEERAVAEAAWRRRRRWGELSVA
  AAAGGGLVGLVCYQLYGDPRAGSPATGRPSKSAATEPEDPPRGRGMLPIPVAAAKETVAIGRTDIEDLDL
  YATSRERRFRLFASIECEGQLFMTPYDFILAVTTDEPKVAKTWKSLSKQELNQMLAETPPVWKGSSKLFR
  NLKEKGVISYTEYLFLLCILTKPHAGFRIAFNMFDTDGNEMVDKKEFLVLQEIFRKKNEKREIKGDEEKR
  AMLRLQLYGYHSPTNSVLKTDAEELVSRSYWDTLRRNTSQALFSDLAERADDITSLVTDTTLLVHFFGKK
  GKAELNFEDFYRFMDNLQTEVLEIEFLSYSNGMNTISEEDFAHILLRYTNVENTSVFLENVRYSIPEEKG
  ITFDEFRSFFQFLNNLEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKFSPHLVNTVFKIFDVDKDDQL
  SYKEFIGIMKDRLHRGFRGYKTVQKYPTFKSCLKKELHSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181723
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181723.3
• RefSeq Size: 4000 bp
• RefSeq ORF: 1593 bp
• Locus ID: 286097
• UniProt ID: Q86XE3
• Cytogenetics: 8p22
• Gene Summary: May play a role in mitochondrial calcium uptake.[UniProtKB/Swiss-Prot Function]