PRKX (NM_005044) Human Tagged ORF Clone

CAT#: RG208332

PRKX (tGFP-tagged) - Human protein kinase, X-linked (PRKX)

Product Images

Circular map for RG208332

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRKX
• Synonyms: PKX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208332 representing NM_005044
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCCCGGGCTGGCCCAGGCGGCCGCGGCGGAGAGCGACTCCCGCAAGGTGGCGGAGGAGACCC
  CCGACGGGGCGCCCGCGCTCTGCCCCAGCCCTGAGGCGCTGTCGCCGGAGCCGCCTGTGTACAGCCTGCA
  GGACTTTGACACGCTGGCCACCGTGGGCACTGGGACGTTCGGGCGGGTGCACCTGGTGAAGGAGAAGACA
  GCCAAGCATTTCTTCGCCCTCAAGGTGATGAGCATTCCTGACGTCATCCGCCTAAAGCAGGAGCAACACG
  TACACAATGAGAAGTCTGTCCTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCA
  TGACGAGCGCTTCCTCTACATGCTCATGGAGTACGTGCCGGGCGGCGAGCTCTTCAGCTACCTGCGCAAC
  CGGGGGCGCTTCTCCAGCACCACGGGGCTCTTCTACTCTGCAGAGATCATCTGTGCCATCGAGTACCTGC
  ACTCCAAAGAGATCGTCTACAGGGACTTGAAGCCAGAGAACATCCTGCTGGATAGGGATGGCCACATTAA
  GCTCACGGACTTTGGGTTCGCCAAGAAGCTGGTAGACAGGACTTGGACCCTCTGTGGAACACCCGAGTAC
  CTAGCCCCCGAAGTCATTCAGAGCAAGGGCCACGGAAGGGCCGTGGACTGGTGGGCCCTCGGCATCCTGA
  TATTCGAGATGCTTTCGGGGTTTCCTCCGTTTTTTGATGACAACCCGTTTGGCATTTATCAGAAAATTCT
  TGCAGGCAAAATAGATTTCCCCAGACATTTGGATTTCCATGTAAAAGACCTCATTAAGAAACTGCTCGTG
  GTTGACAGAACAAGGCGATTAGGAAACATGAAGAACGGGGCGAATGATGTGAAGCATCATCGGTGGTTCC
  GCTCCGTGGACTGGGAAGCTGTTCCGCAGAGAAAACTGAAGCCTCCCATCGTGCCCAAGATAGCTGGTGA
  CGGCGACACTTCCAACTTCGAAACTTACCCTGAGAATGACTGGGACACAGCCGCGCCCGTGCCGCAGAAG
  GATTTAGAAATCTTCAAGAATTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208332 representing NM_005044
  Red=Cloning site Green=Tags(s)
  
  MEAPGLAQAAAAESDSRKVAEETPDGAPALCPSPEALSPEPPVYSLQDFDTLATVGTGTFGRVHLVKEKT
  AKHFFALKVMSIPDVIRLKQEQHVHNEKSVLKEVSHPFLIRLFWTWHDERFLYMLMEYVPGGELFSYLRN
  RGRFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEY
  LAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKIDFPRHLDFHVKDLIKKLLV
  VDRTRRLGNMKNGANDVKHHRWFRSVDWEAVPQRKLKPPIVPKIAGDGDTSNFETYPENDWDTAAPVPQK
  DLEIFKNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005044
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005044.2
• RefSeq Size: 6034 bp
• RefSeq ORF: 1077 bp
• Locus ID: 5613
• UniProt ID: P51817
• Cytogenetics: Xp22.33
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010]