Syntrophin (SNTB2) (NM_006750) Human Tagged ORF Clone

CAT#: RG208309

SNTB2 (tGFP-tagged) - Human syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2) (SNTB2)

Product Images

Circular map for RG208309

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Syntrophin
• Synonyms: D16S2531E; EST25263; SNT2B2; SNT3; SNTL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208309 representing NM_006750
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTAGCTGCGGCGACTGCGGCGGCTGGAGCGGGGCCGGCCATGGCGGTGTGGACGCGGGCCACCA
  AAGCGGGGCTGGTGGAGCTGCTCCTGAGGGAGCGCTGGGTCCGAGTGGTGGCCGAGCTGAGCGGGGAGAG
  CCTGAGCCTGACGGGCGACGCCGCCGCGGCCGAGCTGGAGCCCGCTCTGGGACCCGCGGCCGCCGCCTTC
  AACGGCCTCCCAAACGGCGGCGGCGCGGGCGACTCGCTGCCCGGGAGCCCAAGCCGCGGCCTGGGGCCCC
  CGAGCCCGCCGGCGCCGCCTCGGGGCCCCGCGGGTGAGGCGGGCGCGTCGCCGCCCGTGCGCCGGGTGCG
  GGTGGTGAAGCAAGAGGCGGGCGGCCTGGGCATCAGCATCAAGGGCGGCCGCGAGAACCGGATGCCGATC
  CTCATCTCCAAGATCTTCCCCGGGCTGGCTGCCGACCAGAGCCGGGCGCTGCGGCTGGGCGACGCCATCC
  TGTCGGTGAACGGCACCGACCTGCGCCAGGCCACCCACGACCAGGCCGTGCAGGCGCTGAAGCGCGCGGG
  CAAGGAGGTGCTGCTGGAGGTCAAGTTCATCCGAGAAGTAACACCATATATCAAGAAGCCATCATTAGTA
  TCAGATCTGCCGTGGGAAGGTGCAGCCCCCCAGTCACCAAGCTTTAGTGGCAGTGAGGACTCTGGTTCGC
  CAAAACACCAGAACAGCACCAAGGACAGGAAGATCATCCCTCTCAAAATGTGCTTTGCTGCTAGAAACCT
  AAGCATGCCGGATCTGGAAAACAGATTGATAGAGCTACATTCTCCTGATAGCAGGAACACGTTGATCCTA
  CGCTGCAAAGATACAGCCACAGCACACTCCTGGTTCGTAGCTATCCACACCAACATAATGGCTCTCCTCC
  CACAGGTGTTGGCTGAACTCAACGCCATGCTTGGGGCAACCAGTACAGCAGGAGGCAGTAAAGAGGTGAA
  GCATATTGCCTGGCTGGCAGAACAGGCAAAACTAGATGGTGGAAGACAGCAATGGAGACCTGTCCTCATG
  GCTGTGACTGAGAAGGATTTGCTGCTCTATGACTGTATGCCGTGGACAAGAGATGCCTGGGCGTCACCAT
  GCCACAGCTACCCACTTGTTGCCACCAGGTTGGTTCATTCTGGCTCCGGATGTCGATCCCCCTCCCTTGG
  ATCTGACCTTACATTTGCTACCAGGACAGGCTCTCGACAGGGCATTGAGATGCATCTCTTCAGGGTGGAG
  ACACATCGGGATCTGTCATCCTGGACCAGGATACTTGTTCAGGGTTGCCATGCTGCTGCTGAGCTGATCA
  AGGAAGTCTCTCTAGGCTGCATGTTAAATGGCCAAGAGGTGAGGCTTACTATTCACTATGAAAATGGGTT
  CACCATCTCAAGGGAAAATGGAGGCTCCAGCAGCATATTGTACCGCTACCCCTTTGAAAGGCTGAAGATG
  TCTGCTGATGATGGCATCCGAAATCTATACTTGGATTTTGGTGGTCCCGAGGGAGAACTGACCATGGACC
  TGCACTCTTGTCCGAAGCCGATTGTATTTGTGTTGCACACGTTTTTATCGGCCAAAGTCACTCGTATGGG
  ACTGCTTGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208309 representing NM_006750
  Red=Cloning site Green=Tags(s)
  
  MRVAAATAAAGAGPAMAVWTRATKAGLVELLLRERWVRVVAELSGESLSLTGDAAAAELEPALGPAAAAF
  NGLPNGGGAGDSLPGSPSRGLGPPSPPAPPRGPAGEAGASPPVRRVRVVKQEAGGLGISIKGGRENRMPI
  LISKIFPGLAADQSRALRLGDAILSVNGTDLRQATHDQAVQALKRAGKEVLLEVKFIREVTPYIKKPSLV
  SDLPWEGAAPQSPSFSGSEDSGSPKHQNSTKDRKIIPLKMCFAARNLSMPDLENRLIELHSPDSRNTLIL
  RCKDTATAHSWFVAIHTNIMALLPQVLAELNAMLGATSTAGGSKEVKHIAWLAEQAKLDGGRQQWRPVLM
  AVTEKDLLLYDCMPWTRDAWASPCHSYPLVATRLVHSGSGCRSPSLGSDLTFATRTGSRQGIEMHLFRVE
  THRDLSSWTRILVQGCHAAAELIKEVSLGCMLNGQEVRLTIHYENGFTISRENGGSSSILYRYPFERLKM
  SADDGIRNLYLDFGGPEGELTMDLHSCPKPIVFVLHTFLSAKVTRMGLLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006750
• ORF Size: 1620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006750.3, NP_006741.1
• RefSeq Size: 9771 bp
• RefSeq ORF: 1623 bp
• Locus ID: 6645
• UniProt ID: Q13425
• Cytogenetics: 16q22.1
• Protein Families: Secreted Protein
• Gene Summary: Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008]