KPNA5 (NM_002269) Human Tagged ORF Clone

CAT#: RG208204

KPNA5 (tGFP-tagged) - Human karyopherin alpha 5 (importin alpha 6) (KPNA5)

Product Images

Circular map for RG208204

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KPNA5
• Synonyms: IPOA6; SRP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208204 representing NM_002269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCATGGCTAGTCCAGGGAAAGATAACTATAGAATGAAAAGTTATAAGAATAAAGCCCTAAATC
  CTCAAGAGATGCGTAGACGAAGAGAAGAAGAAGGAATACAGCTTAGAAAACAAAAAAGAGAAGAACAGTT
  GTTCAAACGCAGAAATGTCTATTTGCCCAGAAATGATGAATCTATGCTTGAAAGTCCTATACAGGATCCA
  GATATTAGTTCCACTGTACCCATTCCAGAGGAAGAAGTTGTTACTACAGATATGGTTCAGATGATTTTTT
  CTAATAATGCTGATCAACAGCTAACAGCAACACAGAAATTTAGAAAGCTGCTTTCTAAAGAACCTAATCC
  ACCAATAGATCAAGTTATACAGAAACCAGGAGTTGTACAGAGATTTGTGAAATTTCTTGAAAGAAATGAA
  AATTGCACTTTACAATTTGAAGCTGCATGGGCATTAACAAATATAGCATCTGGAACTTTTCTGCATACCA
  AGGTAGTGATTGAAACTGGGGCTGTTCCGATTTTTATCAAACTTCTTAATTCTGAACATGAAGATGTTCA
  GGAACAGGCTGTTTGGGCACTTGGTAATATTGCTGGTGACAATGCAGAATGCAGAGATTTTGTTTTGAAT
  TGTGAAATACTTCCACCTCTTTTAGAGTTATTAACAAATTCAAACAGACTCACAACAACAAGAAATGCCG
  TGTGGGCCCTCTCAAATTTATGTAGAGGCAAAAACCCTCCTCCAAACTTTAGTAAGGTTTCACCTTGCTT
  AAATGTCCTGTCACGACTGTTGTTTAGCAGTGACCCAGATGTGTTAGCAGACGTGTGTTGGGCCCTTTCT
  TATCTCTCCGATGGACCCAATGATAAAATTCAAGCAGTCATTGATTCTGGAGTCTGTCGAAGATTGGTGG
  AACTTTTGATGCACAATGATTATAAAGTTGTATCACCTGCATTAAGGGCAGTTGGTAATATTGTGACTGG
  TGATGATATTCAAACACAGGTAATTTTGAATTGTTCTGCATTACCCTGTCTCTTACATTTATTGAGTAGC
  CCAAAGGAGTCAATTAGAAAAGAAGCCTGCTGGACTGTTTCTAACATCACTGCTGGAAATAGAGCTCAGA
  TTCAGGCTGTTATAGATGCAAATATTTTTCCTGTTTTGATTGAGATTCTTCAGAAAGCAGAGTTTCGTAC
  CAGAAAAGAAGCAGCTTGGGCTATAACTAATGCAACATCAGGAGGTACTCCAGAGCAAATAAGGTATTTG
  GTAGCTTTAGGCTGCATTAAACCACTTTGTGATCTTTTGACTGTTATGGACTCCAAAATAGTCCAAGTGG
  CTTTAAATGGACTTGAAAATATTTTACGTCTTGGAGAACAAGAATCTAAGCAGAATGGAATAGGCATTAA
  TCCATACTGTGCTCTCATTGAAGAAGCATATGGTCTGGATAAAATTGAGTTTTTGCAAAGCCATGAAAAT
  CAGGAAATTTACCAGAAGGCATTTGATCTGATTGAACATTACTTTGGTGTAGAAGAAGATGACCCCAGCA
  TTGTACCTCAGGTGGATGAAAACCAACAACAGTTTATATTTCAGCAGCAGGAAGCACCAATGGATGGATT
  TCAACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208204 representing NM_002269
  Red=Cloning site Green=Tags(s)
  
  MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDP
  DISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNE
  NCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLN
  CEILPPLLELLTNSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVLADVCWALS
  YLSDGPNDKIQAVIDSGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSS
  PKESIRKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYL
  VALGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQESKQNGIGINPYCALIEEAYGLDKIEFLQSHEN
  QEIYQKAFDLIEHYFGVEEDDPSIVPQVDENQQQFIFQQQEAPMDGFQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002269
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002269.3
• RefSeq Size: 2172 bp
• RefSeq ORF: 1620 bp
• Locus ID: 3841
• UniProt ID: O15131
• Cytogenetics: 6q22.1
• Domains: Armadillo_seg, IBB
• Gene Summary: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]