GTF3C3 (NM_012086) Human Tagged ORF Clone

CAT#: RG208122

GTF3C3 (tGFP-tagged) - Human general transcription factor IIIC, polypeptide 3, 102kDa (GTF3C3), transcript variant 1

Product Images

Circular map for RG208122

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GTF3C3
• Synonyms: TFiiiC2-102; TFIIIC102; TFIIICgamma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208122 representing NM_012086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGGTTCAGTCCGGAACTCATCGACTACTTGGAAGGGAAAATCTCCTTTGAGGAGTTCGAACGGC
  GGAGAGAAGAGAGAAAAACCCGCGAGAAGAAAAGTCTTCAGGAAAAAGGCAAGTTATCAGCTGAAGAAAA
  TCCCGATGACTCTGAAGTTCCATCATCATCAGGAATTAACTCTACCAAATCCCAAGACAAAGATGTCAGT
  GAAGGAGAAACATCAGATGGAGTGAGGAAGTCAGTTCACAAGGTCTTTGCTTCCATGCTTGGAGAGAATG
  AAGATGATGAGGAGGAAGAGGAAGAAGAGGAGGAGGAGGAGGAGGAGGAAGAAACACCTGAGCAACCCAC
  TGCGGGCGATGTATTTGTATTGGAGATGGTTCTCAATCGTGAAACCAAGAAAATGATGAAAGAGAAAAGG
  CCTCGGAGTAAACTTCCCAGAGCTCTGAGAGGTCTCATGGGTGAAGCCAACATTCGTTTTGCTCGAGGAG
  AACGTGAAGAGGCGATATTGATGTGCATGGAAATCATAAGACAAGCTCCTCTGGCTTATGAGCCATTCTC
  TACTCTAGCCATGATATATGAGGACCAAGGTGACATGGAAAAATCATTGCAGTTTGAGTTGATTGCTGCG
  CATTTAAATCCCAGTGACACAGAAGAATGGGTTAGACTGGCAGAAATGTCTCTGGAACAAGACAATATTA
  AGCAGGCTATTTTTTGCTATACAAAAGCTCTTAAATATGAACCTACTAATGTCCGTTATCTGTGGGAGCG
  ATCAAGCCTTTATGAACAGATGGGTGATCATAAAATGGCCATGGATGGTTATAGGCGTATTTTAAACCTT
  TTGTCTCCATCTGATGGCGAACGTTTTATGCAGCTGGCTAGAGATATGGCAAAGAGTTACTATGAAGCCA
  ATGATGTTACTTCTGCTATTAACATAATTGATGAAGCTTTCTCAAAACACCAGGGCCTAGTCTCCATGGA
  AGATGTTAACATAGCAGCTGAACTATATATTTCTAACAAACAGTATGACAAAGCTTTGGAGATAATTACA
  GATTTTTCTGGAATTGTGCTGGAAAAAAAAACTTCAGAAGAAGGCACCTCAGAAGAGAATAAAGCTCCTG
  AGAATGTTACCTGCACTATACCTGATGGCGTGCCAATAGATATCACAGTGAAGTTGATGGTCTGCCTTGT
  ACATCTCAACATTCTTGAACCACTTAATCCTCTCTTGACAACACTAGTAGAACAGAATCCTGAAGATATG
  GGAGACCTATACCTAGATGTTGCTGAAGCTTTTCTGGATGTTGGTGAATATAATTCTGCACTTCCCCTCC
  TCAGTGCTCTTGTTTGCTCTGAAAGATACAACCTTGCAGTAGTTTGGCTTCGTCATGCAGAATGTTTAAA
  GGCCTTAGGCTATATGGAGCGAGCTGCTGAAAGCTATGGCAAGGTGGTTGATCTGGCCCCACTCCATTTG
  GATGCAAGGATTTCACTTTCTACCCTTCAGCAGCAGCTGGGCCAGCCTGAGAAAGCTCTGGAAGCTCTGG
  AACCAATGTATGATCCAGATACTTTAGCACAGGATGCAAATGCTGCACAGCAGGAACTGAAGTTATTGCT
  TCATCGTTCTACTCTGTTGTTTTCACAAGGCAAAATGTATGGTTATGTGGATACCTTACTTACTATGTTA
  GCCATGCTTTTAAAGGTAGCAATGAATCGAGCCCAAGTTTGTTTGATATCCAGTTCCAAGTCTGGAGAGA
  GGCATCTTTATCTTATTAAAGTATCGAGAGACAAAATATCAGACAGCAATGACCAAGAGTCAGCAAATTG
  TGATGCAAAAGCAATATTTGCTGTGCTCACAAGCGTCTTGACAAAGGATGACTGGTGGAATCTTCTGTTG
  AAGGCCATATACTCCTTATGTGACCTATCCCGATTTCAAGAGGCTGAGTTGCTTGTAGATTCCTCATTGG
  AATATTACTCATTTTATGATGACAGGCAAAAACGCAAAGAACTAGAATACTTTGGTCTGTCTGCTGCAAT
  TCTGGACAAAAATTTCAGAAAGGCATACAACTATATCAGGATAATGGTAATGGAAAATGTCAATAAACCC
  CAGCTCTGGAACATTTTCAATCAAGTTACCATGCACTCCCAAGATGTACGACATCATCGCTTCTGTCTCC
  GTTTGATGCTGAAAAACCCAGAAAATCATGCCCTATGTGTCTTAAATGGACACAATGCATTTGTATCTGG
  TAGTTTTAAGCATGCGCTTGGACAGTATGTGCAAGCCTTTCGCACTCACCCTGACGAACCTCTCTATAGC
  TTCTGTATAGGCCTAACCTTTATTCATATGGCATCTCAGAAGTATGTGTTACGGAGACATGCTCTTATTG
  TACAGGGCTTTTCCTTTCTTAATCGATACCTCAGTTTACGTGGGCCCTGCCAGGAATCATTCTACAATTT
  GGGCCGTGGCCTTCATCAGTTGGGGCTGATTCATCTTGCAATCCACTATTATCAGAAGGCCCTGGAGCTC
  CCTCCACTTGTGGTAGAGGGTATAGAACTTGACCAGTTAGACTTACGAAGAGATATTGCCTACAACTTGT
  CTCTCATCTATCAGAGCAGTGGGAATACCGGAATGGCTCAAACGCTTTTGTATACCTATTGTTCTATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208122 representing NM_012086
  Red=Cloning site Green=Tags(s)
  
  MSGFSPELIDYLEGKISFEEFERRREERKTREKKSLQEKGKLSAEENPDDSEVPSSSGINSTKSQDKDVS
  EGETSDGVRKSVHKVFASMLGENEDDEEEEEEEEEEEEEEETPEQPTAGDVFVLEMVLNRETKKMMKEKR
  PRSKLPRALRGLMGEANIRFARGEREEAILMCMEIIRQAPLAYEPFSTLAMIYEDQGDMEKSLQFELIAA
  HLNPSDTEEWVRLAEMSLEQDNIKQAIFCYTKALKYEPTNVRYLWERSSLYEQMGDHKMAMDGYRRILNL
  LSPSDGERFMQLARDMAKSYYEANDVTSAINIIDEAFSKHQGLVSMEDVNIAAELYISNKQYDKALEIIT
  DFSGIVLEKKTSEEGTSEENKAPENVTCTIPDGVPIDITVKLMVCLVHLNILEPLNPLLTTLVEQNPEDM
  GDLYLDVAEAFLDVGEYNSALPLLSALVCSERYNLAVVWLRHAECLKALGYMERAAESYGKVVDLAPLHL
  DARISLSTLQQQLGQPEKALEALEPMYDPDTLAQDANAAQQELKLLLHRSTLLFSQGKMYGYVDTLLTML
  AMLLKVAMNRAQVCLISSSKSGERHLYLIKVSRDKISDSNDQESANCDAKAIFAVLTSVLTKDDWWNLLL
  KAIYSLCDLSRFQEAELLVDSSLEYYSFYDDRQKRKELEYFGLSAAILDKNFRKAYNYIRIMVMENVNKP
  QLWNIFNQVTMHSQDVRHHRFCLRLMLKNPENHALCVLNGHNAFVSGSFKHALGQYVQAFRTHPDEPLYS
  FCIGLTFIHMASQKYVLRRHALIVQGFSFLNRYLSLRGPCQESFYNLGRGLHQLGLIHLAIHYYQKALEL
  PPLVVEGIELDQLDLRRDIAYNLSLIYQSSGNTGMAQTLLYTYCSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012086
• ORF Size: 2658 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012086.1, NP_036218.1
• RefSeq Size: 2961 bp
• RefSeq ORF: 2661 bp
• Locus ID: 9330
• UniProt ID: Q9Y5Q9
• Cytogenetics: 2q33.1
• Domains: TPR
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is part of the TFIIIC2 complex, which binds to the promoters of small nuclear and cytoplasmic RNA genes in order to recruit RNA polymerase III. The TFIIIC2 complex is composed of six subunits. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]