TFAP2E (NM_178548) Human Tagged ORF Clone

CAT#: RG208089

TFAP2E (tGFP-tagged) - Human transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon) (TFAP2E)

Product Images

Circular map for RG208089

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TFAP2E
• Synonyms: AP2E
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208089 representing NM_178548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGCACACCTACTCCGCCATGGAGCGCCCCGACGGGCTGGGAGCAGCTGCCGGCGGGGCCCGCC
  TGTCGTCTCTGCCCCAGGCGGCCTACGGGCCGGCGCCCCCGCTCTGCCACACGCCGGCCGCCACAGCTGC
  CGCCGAATTCCAGCCGCCCTACTTCCCGCCGCCCTACCCGCAGCCACCGCTGCCCTACGGTCAGGCGCCC
  GACGCCGCCGCAGCCTTTCCCCACCTGGCAGGGGACCCATATGGCGGCCTGGCGCCCCTGGCGCAGCCGC
  AGCCTCCTCAGGCCGCCTGGGCCGCGCCCCGCGCAGCCGCCCGCGCCCACGAGGAGCCTCCCGGCCTGCT
  GGCACCGCCCGCCCGCGCCCTGGGCCTTGACCCGCGCCGTGACTATGCCACTGCCGTGCCCCGGCTCCTG
  CACGGCCTGGCCGACGGCGCGCACGGCCTGGCAGACGCACCTCTCGGCCTTCCGGGGCTGGCGGCGGCCC
  CCGGTCTGGAGGACCTGCAGGCAATGGACGAGCCGGGAATGAGCCTCCTAGACCAGTCCGTGATCAAGAA
  AGTGCCCATCCCCTCCAAAGCCAGCAGCCTCTCAGCCCTCTCCTTGGCCAAAGACAGCCTGGTGGGCGGC
  ATCACAAATCCTGGTGAGGTCTTCTGCTCCGTGCCCGGCCGGCTTTCACTGCTCAGCTCAACGTCCAAGT
  ACAAGGTGACGGTGGGGGAGGTGCAGCGGCGACTCTCGCCTCCCGAGTGCCTCAACGCCTCCCTCCTGGG
  GGGTGTCCTCCGCAGGGCCAAGTCCAAAAATGGGGGCCGGTGTTTGCGGGAACGGTTAGAGAAGATTGGG
  CTCAACCTGCCAGCTGGCCGTCGCAAGGCCGCCAATGTGACGCTGCTGACTTCGCTAGTGGAAGGAGAGG
  CCGTGCACCTGGCCCGAGACTTCGGTTACGTCTGTGAGACGGAGTTCCCAGCCAAGGCAGCTGCCGAGTA
  CCTGTGCCGACAGCACGCTGACCCGGGGGAGCTGCACAGCCGCAAGAGCATGCTGCTGGCTGCCAAGCAG
  ATCTGCAAGGAGTTTGCAGACTTGATGGCTCAGGACCGCTCACCGCTGGGCAACAGCCGCCCAGCACTCA
  TCCTGGAGCCCGGAGTACAGAGCTGCTTGACACACTTTAGCCTCATCACCCATGGCTTCGGTGGGCCTGC
  CATCTGTGCTGCCCTCACTGCCTTCCAGAACTATTTGCTGGAGTCACTCAAGGGGCTGGACAAGATGTTT
  CTAAGCAGTGTGGGCAGTGGGCATGGTGAAACCAAGGCTTCGGAGAAGGATGCCAAGCATCGGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208089 representing NM_178548
  Red=Cloning site Green=Tags(s)
  
  MLVHTYSAMERPDGLGAAAGGARLSSLPQAAYGPAPPLCHTPAATAAAEFQPPYFPPPYPQPPLPYGQAP
  DAAAAFPHLAGDPYGGLAPLAQPQPPQAAWAAPRAAARAHEEPPGLLAPPARALGLDPRRDYATAVPRLL
  HGLADGAHGLADAPLGLPGLAAAPGLEDLQAMDEPGMSLLDQSVIKKVPIPSKASSLSALSLAKDSLVGG
  ITNPGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRCLRERLEKIG
  LNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAAAEYLCRQHADPGELHSRKSMLLAAKQ
  ICKEFADLMAQDRSPLGNSRPALILEPGVQSCLTHFSLITHGFGGPAICAALTAFQNYLLESLKGLDKMF
  LSSVGSGHGETKASEKDAKHRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178548
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178548.4
• RefSeq Size: 2189 bp
• RefSeq ORF: 1329 bp
• Locus ID: 339488
• UniProt ID: Q6VUC0
• Cytogenetics: 1p34.3
• Protein Families: Transcription Factors
• Gene Summary: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-epsilon may play a role in the development of the CNS and in cartilage differentiation (By similarity).[UniProtKB/Swiss-Prot Function]