BBS12 (NM_152618) Human Tagged ORF Clone

CAT#: RG207995

BBS12 (tGFP-tagged) - Human Bardet-Biedl syndrome 12 (BBS12), transcript variant 2

Product Images

Circular map for RG207995

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BBS12
• Synonyms: C4orf24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207995 representing NM_152618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGGCTTGCAGAGTCGTAAACAAAAGAAGACACATGGGACTTCAACAACTTTCATCATTCGCGG
  AAACAGGAAGAACTTTCCTAGGCCCACTAAAATCATCCAAATTTATTATAGATGAAGAATGTCATGAAAG
  TGTATTAATCAGTTCAACAGTAAGGCTTCTTGAAAGTTTGGATTTAACCAGTGCAGTGGGACAACTTCTC
  AATGAAGCAGTTCAAGCACAAAACAACACATATAGAACTGGAATCAGTACTCTTTTGTTTCTTGTTGGTG
  CTTGGAGCAGTGCAGTTGAAGAATGTCTTCATCTTGGTGTCCCCATTTCCATAATAGTATCAGTAATGTC
  AGAAGGCTTAAACTTTTGTAGTGAAGAGGTAGTTTCTCTTCATGTACCTGTTCACAATATATTTGACTGT
  ATGGACAGCACAAAAACATTTTCTCAACTTGAAACATTTAGTGTAAGTTTGTGTCCTTTTCTACAGGTCC
  CTTCAGATACTGATTTGATAGAGGAATTGCATGGTCTCAAAGATGTTGCCTCTCAAACACTGACCATTTC
  CAACCTTTCTGGGAGACCTCTTAGATCATATGAATTATTTAAACCTCAGACAAAGGTTGAAGCAGATAAC
  AACACATCACGAACTCTGAAAAACAGCCTGCTTGCAGATACCTGCTGCAGACAGTCAATACTAATCCACA
  GTAGGCATTTTAATAGGACAGATAATACTGAAGGGGTAAGCAAACCAGATGGATTTCAAGAACATGTTAC
  AGCTACTCACAAAACTTACAGATGTAATGATTTGGTAGAGTTGGCAGTAGGCTTGAGTCATGGAGATCAC
  AGCAGCATGAAGTTAGTAGAAGAAGCAGTACAGCTGCAATATCAGAATGCTTGTGTGCAACAAGGCAACT
  GTACAAAACCATTTATGTTTGACATTTCAAGAATTTTCACTTGCTGTCTACCAGGCTTACCTGAAACTTC
  TTCTTGTGTTTGTCCAGGATATATCACTGTTGTGTCAGTATCTAATAATCCTGTGATCAAGGAATTGCAG
  AATCAGCCTGTGCGAATAGTTCTCATTGAGGGTGACCTCACAGAGAATTACCGCCACCTGGGATTTAATA
  AGTCTGCAAATATTAAAACAGTATTAGATAGCATGCAGCTTCAAGAAGACAGCTCAGAAGAACTGTGGGC
  AAATCACGTGTTACAGGTGTTAATCCAGTTCAAGGTGAACCTTGTCCTGGTACAAGGAAATGTGTCCGAA
  CGCTTAATTGAAAAATGTATAAACAGTAAGCGGTTGGTAATCGGCTCAGTGAATGGCAGTGTGATGCAGG
  CTTTTGCAGAGGCTGCAGGAGCAGTACAGGTGGCCTACATTACACAAGTCAATGAAGATTGTGTGGGCAA
  CGGGGTCTGTGTGACCTTCTGGAGAAGCAGCCCTTTGGATGTTGTAGATAGGAACAACAGAATCGCAATC
  TTATTAAAAACAGAAGGAATTAATTTGGTTACGGCCGTGCTCACTAACCCAGTTACTGCACAGATGCAAA
  TCAAAGAAGATAGGTTCTGGACATGTGCCTATCGTTTGTATTATGCTCTAAAAGAGGAAAAGGTCTTCCT
  TGGAGGTGGTGCAGTTGAATTTTTGTGTCTTAGCTGTCTTCATATTCTTGCAGAGCAATCTCTGAAAAAA
  GAAAACCATGCCTGCTCAGGGTGGCTGCATAATACTTCCTCTTGGCTGGCTTCATCTCTGGCAATATACA
  GACCAACTGTGCTTAAATTCCTGGCAAATGGATGGCAGAAATACCTTTCAACTCTCCTATATAACACTGC
  CAATTACTCATCAGAATTTGAAGTCAGCACATACATTCAACATCATCTGCAGAATGCCACAGACTCTGGC
  TCTCCTTCATCTTACATCTTGAATGAATATAGTAAACTAAATAGTAGAATTTTTAATTCAGACATTTCAA
  ATAAACTGGAGCAGATTCCGAGAGTTTATGACGTTGTTACACCAAAGATTGAGGCGTGGCGCCGAGCATT
  GGATTTAGTATTGTTAGTACTTCAGACAGACAGTGAAATAATTACTGGACATGGACACACACAGATAAAT
  TCACAGGAATTAACGGGCTTTCTATTTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207995 representing NM_152618
  Red=Cloning site Green=Tags(s)
  
  MVMACRVVNKRRHMGLQQLSSFAETGRTFLGPLKSSKFIIDEECHESVLISSTVRLLESLDLTSAVGQLL
  NEAVQAQNNTYRTGISTLLFLVGAWSSAVEECLHLGVPISIIVSVMSEGLNFCSEEVVSLHVPVHNIFDC
  MDSTKTFSQLETFSVSLCPFLQVPSDTDLIEELHGLKDVASQTLTISNLSGRPLRSYELFKPQTKVEADN
  NTSRTLKNSLLADTCCRQSILIHSRHFNRTDNTEGVSKPDGFQEHVTATHKTYRCNDLVELAVGLSHGDH
  SSMKLVEEAVQLQYQNACVQQGNCTKPFMFDISRIFTCCLPGLPETSSCVCPGYITVVSVSNNPVIKELQ
  NQPVRIVLIEGDLTENYRHLGFNKSANIKTVLDSMQLQEDSSEELWANHVLQVLIQFKVNLVLVQGNVSE
  RLIEKCINSKRLVIGSVNGSVMQAFAEAAGAVQVAYITQVNEDCVGNGVCVTFWRSSPLDVVDRNNRIAI
  LLKTEGINLVTAVLTNPVTAQMQIKEDRFWTCAYRLYYALKEEKVFLGGGAVEFLCLSCLHILAEQSLKK
  ENHACSGWLHNTSSWLASSLAIYRPTVLKFLANGWQKYLSTLLYNTANYSSEFEVSTYIQHHLQNATDSG
  SPSSYILNEYSKLNSRIFNSDISNKLEQIPRVYDVVTPKIEAWRRALDLVLLVLQTDSEIITGHGHTQIN
  SQELTGFLFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152618
• ORF Size: 2130 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152618.2, NP_689831.2
• RefSeq Size: 3260 bp
• RefSeq ORF: 2133 bp
• Locus ID: 166379
• UniProt ID: Q6ZW61
• Cytogenetics: 4q27
• Gene Summary: The protein encoded by this gene is part of a complex that is involved in membrane trafficking. The encoded protein is a molecular chaperone that aids in protein folding upon ATP hydrolysis. This protein also plays a role in adipocyte differentiation. Defects in this gene are a cause of Bardet-Biedl syndrome type 12. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]