Neuroligin 3 (NLGN3) (NM_018977) Human Tagged ORF Clone

CAT#: RG207955

NLGN3 (tGFP-tagged) - Human neuroligin 3 (NLGN3), transcript variant 2

Product Images

Circular map for RG207955

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Neuroligin 3
• Synonyms: HNL3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207955 representing NM_018977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCGGCTTGGCCCGCCCTCGCTGTCCCTGAGCCCCAAGCCCACGGTTGGCAGGAGCCTGTGCC
  TCACCCTGTGGTTCCTCAGTTTGGCGCTGAGGGCCAGTACCCAGGCCCCAGCACCCACAGTCAACACTCA
  CTTTGGGAAGCTAAGGGGTGCCCGAGTACCACTGCCCAGTGAGATCCTGGGGCCTGTGGACCAATACCTG
  GGGGTGCCCTACGCAGCTCCCCCGATCGGCGAGAAACGTTTCCTGCCCCCTGAACCACCCCCATACTGGT
  CGGGCATCCGGAACGCCACACACTTTCCACCAGTGTGCCCCCAGAACATCCACACAGCTGTGCCCGAAGT
  CATGCTGCCGGTCTGGTTCACTGCCAACTTGGATATCGTCGCTACTTACATCCAGGAGCCCAACGAAGAC
  TGTCTCTACCTGAACGTCTATGTGCCGACGGAGGATGGATCCGGCGCTAAGAAACAGGGCGAGGACTTAG
  CGGATAATGACGGGGATGAAGATGAAGACATCCGGGACAGTGGTGCTAAACCCGTCATGGTCTACATCCA
  CGGAGGCTCTTACATGGAAGGGACAGGCAACATGATTGATGGCAGCATCCTCGCCAGTTATGGCAATGTC
  ATCGTCATCACCCTCAACTATCGGGTTGGAGTGCTAGGTTTCCTGAGTACTGGAGATCAGGCTGCCAAGG
  GCAACTATGGGCTCCTTGACCAGATCCAGGCCCTCCGCTGGGTGAGCGAGAATATTGCCTTCTTCGGGGG
  AGACCCCCGCCGGATCACTGTCTTTGGCTCGGGCATTGGTGCATCCTGCGTCAGCCTCCTAACGTTGTCA
  CATCACTCAGAGGGACTTTTCCAGAGAGCCATCATCCAAAGTGGCTCTGCTCTGTCCAGCTGGGCTGTGA
  ACTACCAACCAGTGAAGTACACCAGCCTGCTGGCAGACAAAGTGGGCTGTAATGTGCTGGACACCGTGGA
  TATGGTGGACTGTCTTCGGCAAAAGAGTGCCAAGGAGCTGGTAGAGCAGGACATCCAGCCAGCCCGCTAC
  CACGTGGCCTTTGGCCCTGTGATTGATGGTGATGTCATTCCTGATGACCCTGAGATCCTCATGGAGCAGG
  GCGAGTTCCTCAACTATGACATCATGCTAGGTGTCAACCAGGGCGAGGGTCTCAAGTTTGTGGAAGGGGT
  GGTGGACCCTGAGGATGGTGTCTCTGGCACTGACTTTGACTATTCCGTCTCCAATTTTGTGGACAATCTG
  TATGGCTATCCTGAGGGTAAGGACACCCTGCGAGAGACCATCAAGTTCATGTATACAGACTGGGCAGACC
  GTGACAACCCTGAGACCCGCCGTAAAACACTGGTGGCACTCTTCACTGACCACCAGTGGGTGGAGCCCTC
  AGTGGTGACAGCCGATCTGCATGCCCGCTACGGCTCGCCTACCTACTTCTACGCCTTCTATCATCACTGC
  CAGAGCCTCATGAAGCCTGCTTGGTCAGATGCAGCTCATGGGGATGAAGTACCCTATGTTTTTGGGGTTC
  CTATGGTAGGCCCCACTGACCTTTTCCCCTGCAACTTCTCCAAGAATGATGTTATGCTCAGTGCTGTCGT
  CATGACCTATTGGACCAACTTTGCCAAGACTGGGGATCCCAACAAGCCGGTCCCCCAGGACACCAAGTTC
  ATTCACACCAAGGCCAACCGCTTTGAGGAAGTGGCCTGGTCCAAATACAATCCCCGAGACCAGCTCTACC
  TTCACATCGGGCTGAAACCAAGGGTCCGAGATCATTACCGGGCCACTAAGGTGGCCTTTTGGAAACATCT
  GGTGCCGCACCTATACAACCTGCATGACATGTTCCACTATACGTCCACCACCACCAAAGTGCCGCCTCCG
  GATACCACCCACAGCTCCCACATCACCCGCAGGCCCAATGGCAAGACCTGGAGCACCAAGCGGCCAGCCA
  TCTCACCTGCCTACAGCAACGAGAATGCCCAGGGGTCCTGGAACGGGGACCAGGATGCAGGGCCACTCCT
  GGTGGAGAACCCTCGTGACTACTCCACTGAATTAAGTGTCACCATCGCCGTGGGGGCCTCCATCCTGTTC
  CTTAACGTTCTGGCCTTCGCTGCCCTCTACTACCGTAAGGACAAACGGCGCCAGGAGCCCCTGCGGCAGC
  CTAGCCCTCAGCGGGGAGCCTGGGCCCCGGAGTTGGGAGCTGCTCCAGAGGAGGAGCTGGCAGCATTACA
  ACTGGGCCCCACCCACCACGAGTGTGAGGCCAGTCCCCCCCATGACACGCTGCGCCTCACTGCATTGCCC
  GACTACACCCTGACCCTGCGGCGCTCCCCGGATGACATCCCACTCATGACCCCCAACACCATCACTATGA
  TCCCCAACTCCCTGGTAGGGCTGCAGACATTGCACCCCTATAACACCTTTGCCGCAGGGTTCAACAGTAC
  CGGGCTGCCCCACTCACACTCCACTACCCGGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207955 representing NM_018977
  Red=Cloning site Green=Tags(s)
  
  MWLRLGPPSLSLSPKPTVGRSLCLTLWFLSLALRASTQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYL
  GVPYAAPPIGEKRFLPPEPPPYWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNED
  CLYLNVYVPTEDGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSILASYGNV
  IVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLS
  HHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARY
  HVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNL
  YGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHC
  QSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKF
  IHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPP
  DTTHSSHITRRPNGKTWSTKRPAISPAYSNENAQGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASILF
  LNVLAFAALYYRKDKRRQEPLRQPSPQRGAWAPELGAAPEEELAALQLGPTHHECEASPPHDTLRLTALP
  DYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018977
• ORF Size: 2484 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018977.2, NP_061850.2
• RefSeq Size: 3905 bp
• RefSeq ORF: 2487 bp
• Locus ID: 54413
• UniProt ID: Q9NZ94
• Cytogenetics: Xq13.1
• Domains: COesterase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Oct 2009]