NSMAF (NM_003580) Human Tagged ORF Clone

CAT#: RG207925

NSMAF (tGFP-tagged) - Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1

Product Images

Circular map for RG207925

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NSMAF
• Synonyms: FAN; GRAMD5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207925 representing NM_003580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGATTTTCCTTGC
  TGCTGCTTAACTTGGAGGAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCA
  CCATGAAAGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCAATA
  TCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGAGAAAATGGAGCCA
  ATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTCAGTCAGGTATATTTCATTAAAGA
  ACATAATGTTGTTGCACCATATAAAATAGAAAGGGGCAAAATGGAATATGTTTTTGAATTGGATGTTCCC
  GGGAAAGTGGAAGATGTTGTGGAGACGTTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTG
  ACCAAACCGCCATGATAACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTT
  CCAAAACATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAATCCT
  GGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCGAAACCTGTGGTCC
  AGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGCCTCATGCCTCTGGGCTTGGAAGT
  ATTTTGCACAGAAGATGATCTGTGTTCCGACATCTACCTAAAGTTCTATGAACCTCAAGATAGAGATGAT
  CTCTATTTTTACATTGCCACATACCTAGAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGC
  AGTGGCAGCGTGGACACCTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTG
  CAACGACCTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGATTTG
  TCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAACGGCTGGAGAGAC
  TACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGGAGTCACTACTCTTCCCCGGGTTA
  TGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTATATGCTGTGCCTGCAGAATGGAAGATTTGATAAT
  GCAGATAGAATGTTCAACAGTATTGCAGAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAG
  AGTTAATTCCAGAATTCTATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAA
  GAGACAAGGAGGACAGATGGTTGACGATGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTTCTC
  CAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGGATTGATCTAATAT
  TTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTATTTCATCCCCTGACCTATGAAGG
  AGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAGAAGGTAGCCATGCTTACGCAAATCTTGGAATTT
  GGGCAGACACCAAAACAACTATTTGTGACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGT
  CCCAGACCTCCAGTTATAATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGA
  AGAAAGCAAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCACAAA
  GAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCCCAAGATTCCACCT
  TGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCATTTTCAAATATGGCTTTATCGTC
  TTGTTTACTTTTACCAGGAGATGCCACTGTCATAACTTCTTCATGGGATAATAATGTCTATTTTTATTCC
  ATAGCATTTGGAAGACGCCAGGACACGTTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATG
  ACAACAGGCTATATTCTGCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCC
  AGGCACCAAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATCAGT
  TTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATTTGGGACCTCACAA
  CGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGTGACACTGCTTTTAGCCCAGATAG
  TCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTTAATGTCATTGATGTGCAGACAGGAATGCTCATC
  TCCTCCATGACATCAGATGAGCCCCAGAGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTC
  AGTCTGGTGAACTGCTCGTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGG
  TGCTGTGACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAAATT
  ATATTCTGGAAATTGCAGTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207925 representing NM_003580
  Red=Cloning site Green=Tags(s)
  
  MAFIRKKQQEQQLQLYSKERFSLLLLNLEEYYFEQHRANHILHKGSHHERKIRGSLKICSKSVIFEPDSI
  SQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIFSQVYFIKEHNVVAPYKIERGKMEYVFELDVP
  GKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLARTSFDKNRFQNISEKLHMECKAEMVTPLVTNP
  GHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDD
  LYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIHDYSSSELDL
  SNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDN
  ADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELPPWASSPEDFL
  QKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIQDPDEKVAMLTQILEF
  GQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHK
  EAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYS
  IAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTIS
  LNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLI
  SSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQI
  IFWKLQY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003580
• ORF Size: 2751 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003580.2, NP_003571.2
• RefSeq Size: 3564 bp
• RefSeq ORF: 2754 bp
• Locus ID: 8439
• UniProt ID: Q92636
• Cytogenetics: 8q12.1
• Domains: Beach, WD40, GRAM
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]