Netrin 5 (NTN5) (NM_145807) Human Tagged ORF Clone

CAT#: RG207703

NTN5 (tGFP-tagged) - Human netrin 5 (NTN5)

Product Images

Circular map for RG207703

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Netrin 5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207703 representing NM_145807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGTGACCTTTGCCCTCCTGCTCCTCCTGGGCCAGGCCACTGCGGACCCATGCTACGATCCACAGG
  GCCGCCCCCAATTCTGCCTCCCGCCAGTGACACAGCTGGCTGCCGTGGCGGCCTCCTGCCCTCAGGCCTG
  TGCCCTGTCCCCAGGAAACCACCTTGGCGCCAGGGAAACCTGCAATGGCAGCCTGACTTTGGCCCTGGGT
  GGCCCCTTCCTCCTGACATCTGTCAGCTTGCGCTTCTGTACCCCAGGACCCCCAGCCCTCATCCTGTCTG
  CTGCCTGGGCCTCAGGGGGTCCCTGGAGGCTGCTGTGGCACAGGCCCGCCTGGCCTGGGGCCTTAGGGGG
  CCCTGAAAGGGTGACCTTCCACTCCACACCAGGTCCTAAGGCCACTGTGGCGGCCAGCCACCTCCGTGTG
  GAGTTTGGGGGCCAGGCCGGGCTAGCGGCAGCTGGGCTGAGAGGCCGCTGCCAGTGTCATGGCCACGCTG
  CCCGCTGTGCCGCCCGTGCCCGGCCCCCCCGCTGCCACTGCCGCCACCATACCACTGGCCCGGGGTGCGA
  GAGCTGCCGCCCGTCCCATCGAGACTGGCCCTGGCGGCCTGCCACGCCCCGGCACCCCCACCCTTGCCTA
  CCCTGCTCCTGCAACCAGCACGCCCGACGCTGCCGGTTCAACTCTGAGCTGTTCAGACTGTCGGGCGGCC
  GGAGTGGGGGTGTTTGTGAGCGGTGCCGCCACCACACAGCTGGGCGGCACTGCCACTACTGCCAACCTGG
  GTTCTGGAGGGACCCTAGCCAGCCTATCTTCAGCCGCAGGGCCTGCAGAGCCTGCCAGTGCCACCCTATT
  GGGGCAACAGGAGGAACCTGCAACCAGACCAGTGGGCAGTGCACCTGCAAGTTAGGGGTCACAGGCCTGA
  CCTGCAACCGCTGTGGCCCTGGCTACCAGCAGAGCCGCTCCCCCAGGATGCCCTGCCAGCGAATTCCAGA
  GGCAACAACCACCCTTGCCACTACTCCTGGTGCTTATAGCTCTGACCCTCAGTGTCAAAACTACTGCAAT
  ATGTCGGACACCAGGGTACACATGAGCCTTCGGAGGTACTGCCAGCAGGACCATGTTCTCCGCGCGCAGG
  TGCTAGCGTCCGAGGCGGCGGGCCCGGCATGGCAGCGGCTGGCCGTGCGCGTGCTGGCCGTTTACAAGCA
  GCGGGCGCAGCCCGTGCGACGCGGCGACCAGGACGCCTGGGTGCCCCGCGCCGACCTGACCTGCGGCTGC
  CTGCGCCTGCAGCCAGGCACCGACTACCTGCTGCTGGGCAGCGCCGTGGGCGACCCCGACCCCACGCGCC
  TCATCCTCGACCGCCACGGCCTCGCGCTGCCATGGAGGCCGCGCTGGGCCCGGCCCCTGAAGCGGCTGCA
  GCAGGAGGAGCGCGCCGGAGGCTGCCGCGGCGTGCGGGCACCCACACCCAGCCCCAGGCCGGAGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207703 representing NM_145807
  Red=Cloning site Green=Tags(s)
  
  MPVTFALLLLLGQATADPCYDPQGRPQFCLPPVTQLAAVAASCPQACALSPGNHLGARETCNGSLTLALG
  GPFLLTSVSLRFCTPGPPALILSAAWASGGPWRLLWHRPAWPGALGGPERVTFHSTPGPKATVAASHLRV
  EFGGQAGLAAAGLRGRCQCHGHAARCAARARPPRCHCRHHTTGPGCESCRPSHRDWPWRPATPRHPHPCL
  PCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPSQPIFSRRACRACQCHPI
  GATGGTCNQTSGQCTCKLGVTGLTCNRCGPGYQQSRSPRMPCQRIPEATTTLATTPGAYSSDPQCQNYCN
  MSDTRVHMSLRRYCQQDHVLRAQVLASEAAGPAWQRLAVRVLAVYKQRAQPVRRGDQDAWVPRADLTCGC
  LRLQPGTDYLLLGSAVGDPDPTRLILDRHGLALPWRPRWARPLKRLQQEERAGGCRGVRAPTPSPRPEH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145807
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145807.3
• RefSeq Size: 1898 bp
• RefSeq ORF: 1470 bp
• Locus ID: 126147
• UniProt ID: Q8WTR8
• Cytogenetics: 19q13.33
• Gene Summary: Plays a role in neurogenesis. Prevents motor neuron cell body migration out of the neural tube.[UniProtKB/Swiss-Prot Function]