Kv beta 1 (KCNAB1) (NM_172159) Human Tagged ORF Clone
KCNAB1 (GFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3
|Product Name||Kv beta 1 (KCNAB1) (NM_172159) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||AKR6A3; hKvb3; hKvBeta3; KCNA1B; KV-BETA-1; Kvb1.3|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG207384 representing NM_172159
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG207384 representing NM_172159
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1203 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||4094 bp|
|RefSeq ORF||1206 bp|
|Protein Families||Druggable Genome, Ion Channels: Other|
|Gene Summary||Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member includes distinct isoforms which are encoded by alternatively spliced transcript variants of this gene. Some of these isoforms are beta subunits, which form heteromultimeric complexes with alpha subunits and modulate the activity of the pore-forming alpha subunits. [provided by RefSeq, Apr 2015]|
|cDNA Clone Resources|
|SC109907||KCNAB1 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3||
|RC207384||KCNAB1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3||
|RC207384L1||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3, Myc-DDK-tagged||
|RC207384L2||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3, mGFP tagged||
|RC207384L3||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3, Myc-DDK-tagged||
|RC207384L4||Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 3, mGFP tagged||