MXI1 (NM_130439) Human Tagged ORF Clone

CAT#: RG207319

MXI1 (tGFP-tagged) - Human MAX interactor 1 (MXI1), transcript variant 2

Product Images

Circular map for RG207319

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MXI1
• Synonyms: bHLHc11; MAD2; MXD2; MXI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207319 representing NM_130439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAACGCGGGCGGCCGCGCAAGGAGGCGCGCTGCGAGGGCGCGGGGCTGGCCCCCGCCGCGCCCC
  CGGCTGTGCCCCCCGCCGTGGCCGCGCCCCAGCCCCCGGCCCTGCCCGAGGACCCCGCTGGGGCCAAGCC
  CAGGTGCCCCTTCTCAGACATTTTCAACACCAGCGAGAACTCGATGGAGAAGCACATCAACACTTTTCTG
  CAGAACGTGCAGATTCTGCTCGAGGCCGCCAGCTACCTGGAGCAGATCGAGAAAGAAAACAAAAAGTGTG
  AACATGGCTACGCCTCTTCATTCCCGTCCATGCCGAGCCCCCGACTGCAGCATTCAAAGCCCCCACGGAG
  GTTGAGCCGGGCACAGAAACACAGCAGCGGGAGCAGCAACACCAGCACTGCCAACAGATCTACACACAAT
  GAGCTGGAAAAGAATCGACGAGCTCATCTGCGCCTTTGTTTAGAACGCTTAAAAGTTCTGATTCCACTAG
  GACCAGACTGCACCCGGCACACAACACTTGGTTTGCTCAACAAAGCCAAAGCACACATCAAGAAACTTGA
  AGAAGCTGAAAGAAAAAGCCAGCACCAGCTCGAGAATTTGGAACGAGAACAGAGATTTTTAAAGTGGCGA
  CTGGAACAGCTGCAGGGTCCTCAGGAGATGGAACGAATACGAATGGACAGCATTGGATCAACTATTTCTT
  CAGATCGTTCTGATTCAGAGCGAGAGGAGATTGAAGTGGATGTTGAAAGCACAGAGTTCTCCCATGGAGA
  AGTGGACAATATAAGTACCACCAGCATCAGTGACATTGATGACCACAGCAGCCTGCCGAGTATTGGGAGT
  GACGAGGGTTACTCCAGTGCCAGTGTCAAACTTTCATTCACTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207319 representing NM_130439
  Red=Cloning site Green=Tags(s)
  
  MGKRGRPRKEARCEGAGLAPAAPPAVPPAVAAPQPPALPEDPAGAKPRCPFSDIFNTSENSMEKHINTFL
  QNVQILLEAASYLEQIEKENKKCEHGYASSFPSMPSPRLQHSKPPRRLSRAQKHSSGSSNTSTANRSTHN
  ELEKNRRAHLRLCLERLKVLIPLGPDCTRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWR
  LEQLQGPQEMERIRMDSIGSTISSDRSDSEREEIEVDVESTEFSHGEVDNISTTSISDIDDHSSLPSIGS
  DEGYSSASVKLSFTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130439
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130439.3, NP_569157.2
• RefSeq Size: 3470 bp
• RefSeq ORF: 888 bp
• Locus ID: 4601
• UniProt ID: P50539
• Cytogenetics: 10q25.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]