ALDH5A1 (NM_001080) Human Tagged ORF Clone

CAT#: RG207213

ALDH5A1 (tGFP-tagged) - Human aldehyde dehydrogenase 5 family, member A1 (ALDH5A1), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RG207213

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH5A1
• Synonyms: SSADH; SSDH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207213 representing NM_001080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCTGCATTTGGCTGCGGAGCTGTGGGGCCCGGCGCCTCGGGTCGACGTTTCCAGGCTGCCGCC
  TCCGCCCCCGCGCCGGCGGCCTGGTCCCTGCCTCCGGGCCTGCGCCCGGCCCGGCCCAGCTCCGCTGCTA
  CGCTGGGCGCCTGGCGGGCCTCTCTGCGGCGCTGCTGCGCACCGACAGCTTCGTGGGCGGCCGCTGGCTC
  CCGGCCGCCGCCACCTTCCCCGTGCAAGACCCGGCCAGCGGCGCCGCTCTGGGCATGGTAGCCGACTGCG
  GGGTGCGAGAGGCCCGCGCCGCCGTGCGCGCTGCCTACGAGGCTTTCTGCCGCTGGAGGGAGGTCTCCGC
  CAAGGAGAGGAGTTCATTACTTCGGAAGTGGTACAATTTAATGATACAAAATAAGGATGACCTTGCCAGA
  ATAATCACAGCTGAAAGTGGAAAGCCACTGAAGGAGGCACATGGAGAAATTCTCTATTCCGCCTTTTTCC
  TAGAGTGGTTCTCTGAGGAAGCCCGCCGTGTTTACGGAGACATTATCCACACCCCGGCAAAGGACAGGCG
  GGCCCTGGTCCTCAAGCAGCCCATAGGCGTGGCTGCAGTCATCACCCCGTGGAATTTCCCCAGTGCCATG
  ATCACCCGGAAGGTGGGGGCCGCCCTGGCAGCCGGCTGTACTGTCGTGGTGAAGCCTGCCGAAGACACGC
  CCTTCTCCGCCCTGGCCCTGGCTGAGCTTGCAAGCCAGGCTGGGATTCCTTCAGGTGTATACAATGTTAT
  TCCCTGTTCTCGAAAGAATGCCAAGGAAGTAGGGGAGGCAATTTGTACTGATCCTCTGGTGTCCAAAATT
  TCCTTTACTGGTTCAACAACTACAGGAAAGATCCTGTTGCACCACGCAGCAAACTCTGTGAAAAGGGTCT
  CTATGGAGCTGGGCGGCCTTGCTCCATTTATAGTATTTGACAGTGCCAACGTGGACCAGGCTGTAGCAGG
  GGCCATGGCATCTAAATTTAGGAACACTGGACAGACTTGTGTTTGCTCAAACCAATTCTTGGTGCAAAGG
  GGCATCCATGATGCCTTTGTAAAAGCATTCGCCGAGGCCATGAAGAAGAACCTGCGCGTAGGTAATGGAT
  TTGAGGAAGGAACTACTCAGGGCCCATTAATTAATGAAAAAGCGGTAGAAAAGGTGGAGAAACAGGTGAA
  TGATGCCGTTTCTAAAGGTGCCACCGTTGTGACAGGTGGAAAACGACACCAACTTGGAAAAAATTTCTTT
  GAGCCTACCCTGCTGTGCAATGTCACCCAGGACATGCTGTGCACTCATGAAGAGACTTTCGGGCCTCTGG
  CACCAGTTATCAAGTTCGATACAGAGGAGGAGGCTATAGCAATCGCTAACGCAGCTGATGTTGGGTTAGC
  AGGTTATTTTTACTCTCAAGACCCAGCCCAGATCTGGAGAGTGGCAGAGCAGCTGGAAGTGGGCATGGTT
  GGCGTCAACGAAGGATTAATTTCCTCTGTGGAGTGCCCTTTTGGTGGAGTGAAGCAGTCCGGCCTTGGGC
  GAGAGGGGTCCAAGTATGGCATTGATGAGTATCTGGAACTCAAGTATGTGTGTTACGGGGGCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207213 representing NM_001080
  Red=Cloning site Green=Tags(s)
  
  MATCIWLRSCGARRLGSTFPGCRLRPRAGGLVPASGPAPGPAQLRCYAGRLAGLSAALLRTDSFVGGRWL
  PAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLAR
  IITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAM
  ITRKVGAALAAGCTVVVKPAEDTPFSALALAELASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPLVSKI
  SFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQR
  GIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFF
  EPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMV
  GVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080.3, NP_001071.1
• RefSeq Size: 5131 bp
• RefSeq ORF: 1608 bp
• Locus ID: 7915
• UniProt ID: P51649
• Cytogenetics: 6p22.3
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Butanoate metabolism, Metabolic pathways
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]