HEF1 (NEDD9) (NM_006403) Human Tagged ORF Clone

CAT#: RG207200

NEDD9 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1

Product Images

Circular map for RG207200

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HEF1
• Synonyms: CAS-L; CAS2; CASL; CASS2; HEF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207200 representing NM_006403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAGGAACTGGCCT
  TTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTGGAAGGATGGTGGCTGTGCTC
  GTTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACT
  GCCTCCAGTCACGAGCAGCCTGCCTCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAG
  TGCCAAACCCACAGGCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAAT
  TTACCAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA
  AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACAGGCCATGGCT
  ACGTATACGAGTACCCATCCAGATACCAAAAGGACGTCTATGATATCCCTCCTTCTCATACCACTCAAGG
  GGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATAAAACCT
  CAAGGGGTGTATGACATCCCGCCTACAAAAGGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAG
  CAGGGCTTAGGGAAAAAGACTATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACC
  GGAGGGGGTTTATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAAC
  TGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAATCACCCACCCC
  CGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCCCGAGGCGTTCAGTTTCTTGA
  GCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAAAGGGATGGTGTTTATGATGTCCCTCTGCAT
  AACCCGCCAGATGCTAAAGGCTCTCGGGACTTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAG
  GCAGCACCCGGAGTAACATGTCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGC
  TCAGGACAAAAGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTT
  GAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATATATGGAAAGAC
  ACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAGGAGTACCTCCACTTTGTCAA
  GGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATCCTCCACAACAAGATGAAGCGGGAGCTGCAA
  CGAGTTGAAGACTCCCACCAGATCCTGAGTCAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGA
  ATATCTTGGCCATCAACAAGCCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGAC
  GGTGCCCGATGACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGC
  CCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCACACGGTGGCT
  CCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAACAAGGCACTGCCCCCAGGCCT
  GAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGTTCTGAGAGGAGCTGGATGGATGACTACGAT
  TACGTCCACCTACAGGGTAAGGAGGAGTTTGAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCA
  TGAAACAGAACAAGATGCAGCTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTAC
  AAAGCCCGTGGAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTG
  AGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATTTCCCTTCTCA
  ACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGAATCTTCGTGGCACACAGCAA
  GTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGAGACACGCTGACACGGCAGGTGACTGCCCAG
  GACATTCGCAACAAAGTCATGAACTCCAGCAACCAGCTCTGCGAGCAGCTCAAGACCATAGTCATGGCAA
  CCAAGATGGCCGCCCTCCATTACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCT
  TTCTAGAAATGCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207200 representing NM_006403
  Red=Cloning site Green=Tags(s)
  
  MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPMQET
  ASSHEQPASGLMQQTFGQQKLYQVPNPQAAPRDTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQR
  SIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKP
  QGVYDIPPTKGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYN
  CDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLH
  NPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQAL
  EMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQ
  RVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPG
  PGSLHLKNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERSWMDDYD
  YVHLQGKEEFERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGV
  SAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQ
  DIRNKVMNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006403
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006403.2, NP_006394.1
• RefSeq Size: 4532 bp
• RefSeq ORF: 2505 bp
• Locus ID: 4739
• UniProt ID: Q14511
• Cytogenetics: 6p24.2
• Domains: SH3
• Gene Summary: The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]