CD5 (NM_014207) Human Tagged ORF Clone

CAT#: RG206494

CD5 (tGFP-tagged) - Human CD5 molecule (CD5)

Product Images

Circular map for RG206494

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD5
• Synonyms: LEU1; T1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206494 representing NM_014207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCGCTTCCTGCCTCG
  GACGGCTCAGCTGGTATGACCCAGATTTCCAGGCAAGGCTCACCCGTTCCAACTCGAAGTGCCAGGGCCA
  GCTGGAGGTCTACCTCAAGGACGGATGGCACATGGTTTGCAGCCAGAGCTGGGGCCGGAGCTCCAAGCAG
  TGGGAGGACCCCAGTCAAGCGTCAAAAGTCTGCCAGCGGCTGAACTGTGGGGTGCCCTTAAGCCTTGGCC
  CCTTCCTTGTCACCTACACACCTCAGAGCTCAATCATCTGCTACGGACAACTGGGCTCCTTCTCCAACTG
  CAGCCACAGCAGAAATGACATGTGTCACTCTCTGGGCCTGACCTGCTTAGAACCCCAGAAGACAACACCT
  CCAACGACAAGGCCCCCGCCCACCACAACTCCAGAGCCCACAGCTCCTCCCAGGCTGCAGCTGGTGGCAC
  AGTCTGGCGGCCAGCACTGTGCCGGCGTGGTGGAGTTCTACAGCGGCAGCCTGGGGGGTACCATCAGCTA
  TGAGGCCCAGGACAAGACCCAGGACCTGGAGAACTTCCTCTGCAACAACCTCCAGTGTGGCTCCTTCTTG
  AAGCATCTGCCAGAGACTGAGGCAGGCAGAGCCCAAGACCCAGGGGAGCCACGGGAACACCAGCCCTTGC
  CAATCCAATGGAAGATCCAGAACTCAAGCTGTACCTCCCTGGAGCATTGCTTCAGGAAAATCAAGCCCCA
  GAAAAGTGGCCGAGTTCTTGCCCTCCTTTGCTCAGGTTTCCAGCCCAAGGTGCAGAGCCGTCTGGTGGGG
  GGCAGCAGCATCTGTGAAGGCACCGTGGAGGTGCGCCAGGGGGCTCAGTGGGCAGCCCTGTGTGACAGCT
  CTTCAGCCAGGAGCTCGCTGCGGTGGGAGGAGGTGTGCCGGGAGCAGCAGTGTGGCAGCGTCAACTCCTA
  TCGAGTGCTGGACGCTGGTGACCCAACATCCCGGGGGCTCTTCTGTCCCCATCAGAAGCTGTCCCAGTGC
  CACGAACTTTGGGAGAGAAATTCCTACTGCAAGAAGGTGTTTGTCACATGCCAGGATCCAAACCCCGCAG
  GCCTGGCCGCAGGCACGGTGGCAAGCATCATCCTGGCCCTGGTGCTCCTGGTGGTGCTGCTGGTCGTGTG
  CGGCCCCCTTGCCTACAAGAAGCTAGTGAAGAAATTCCGCCAGAAGAAGCAGCGCCAGTGGATTGGCCCA
  ACGGGAATGAACCAAAACATGTCTTTCCATCGCAACCACACGGCAACCGTCCGATCCCATGCTGAGAACC
  CCACAGCCTCCCACGTGGATAACGAATACAGCCAACCTCCCAGGAACTCCCGCCTGTCAGCTTATCCAGC
  TCTGGAAGGGGTTCTGCATCGCTCCTCCATGCAGCCTGACAACTCCTCCGACAGTGACTATGATCTGCAT
  GGGGCTCAGAGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206494 representing NM_014207
  Red=Cloning site Green=Tags(s)
  
  MPMGSLQPLATLYLLGMLVASCLGRLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQ
  WEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTP
  PTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFL
  KHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVG
  GSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQC
  HELWERNSYCKKVFVTCQDPNPAGLAAGTVASIILALVLLVVLLVVCGPLAYKKLVKKFRQKKQRQWIGP
  TGMNQNMSFHRNHTATVRSHAENPTASHVDNEYSQPPRNSRLSAYPALEGVLHRSSMQPDNSSDSDYDLH
  GAQRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014207
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014207.2, NP_055022.1
• RefSeq Size: 3151 bp
• RefSeq ORF: 1488 bp
• Locus ID: 921
• UniProt ID: P06127
• Cytogenetics: 11q12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage
• Gene Summary: This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]