KLHDC10 (NM_014997) Human Tagged ORF Clone

CAT#: RG206230

KLHDC10 (tGFP-tagged) - Human kelch domain containing 10 (KLHDC10)

Product Images

Circular map for RG206230

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHDC10
• Synonyms: slim
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206230 representing NM_014997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGCCCAGGGCTGGGACAGGAACCGCCGGAGGGGAGGAGGCGCCGCCGGCGCTGGTGGCGGAG
  GTAGCGGGGCCGGCGGGGGCAGTGGGGGCAGCGGGGGTCGGGGGACTGGCCAGCTCAACCGCTTCGTGCA
  ACTCTCCGGGCGGCCGCACCTGCCAGGTAAGAAGAAAATACGATGGGACCCAGTTAGGAGGCGCTTCATT
  CAGTCCTGTCCCATCATAAGGATCCCTAACAGGTTTTTGAGAGGCCACAGACCTCCACCAGCACGAAGTG
  GACATCGTTGTGTGGCAGATAATACCAACCTATATGTGTTTGGAGGTTATAACCCAGATTATGATGAATC
  GGGAGGGCCTGATAATGAAGACTATCCTCTCTTCAGGGAACTCTGGAGGTATCATTTTGCTACAGGAGTA
  TGGCACCAGATGGGCACAGATGGCTACATGCCCCGGGAATTGGCATCTATGTCACTTGTGCTGCATGGAA
  ACAACCTGTTAGTATTTGGAGGTACGGGCATCCCATTTGGAGAGAGCAACGGCAATGACGTCCATGTGTG
  TAATGTGAAGTATAAGAGATGGGCTTTGCTCAGCTGTCGGGGGAAGAAACCCAGTCGTATATATGGACAG
  GCTATGGCCATCATCAATGGCTCCCTTTATGTCTTTGGAGGTACAACCGGCTATATTTACAGCACAGACC
  TGCACAAGTTAGATCTCAATACCAGAGAGTGGACACAACTGAAACCAAACAACCTATCCTGTGATCTACC
  AGAAGAGAGATACCGACATGAAATTGCACATGACGGGCAGAGGATTTACATCTTGGGAGGTGGTACTTCC
  TGGACAGCATATTCCTTAAACAAGATCCATGCATACAACCTTGAAACGAATGCCTGGGAGGAAATTGCAA
  CAAAACCCCATGAAAAAATAGGCTTTCCTGCAGCCCGAAGGTGTCACAGTTGTGTTCAAATAAAAAATGA
  TGTATTTATTTGTGGGGGCTATAATGGAGAGGTGATCCTGGGAGATATCTGGAAGTTGAATCTGCAGACT
  TTCCAATGGGTGAAGCTCCCAGCTACCATGCCAGAGCCAGTTTATTTTCACTGTGCAGCTGTTACACCAG
  CTGGTTGCATGTACATTCATGGAGGAGTGGTGAACATCCATGAAAACAAACGGACTGGGTCATTGTTTAA
  GATCTGGCTGGTGGTACCTAGCCTGCTGGAACTGGCATGGGAGAAGCTGCTTGCGGCCTTCCCTAACCTT
  GCAAACCTCTCCCGAACACAACTTCTGCACCTTGGACTCACACAGGGACTCATCGAACGCTTGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206230 representing NM_014997
  Red=Cloning site Green=Tags(s)
  
  MSAAQGWDRNRRRGGGAAGAGGGGSGAGGGSGGSGGRGTGQLNRFVQLSGRPHLPGKKKIRWDPVRRRFI
  QSCPIIRIPNRFLRGHRPPPARSGHRCVADNTNLYVFGGYNPDYDESGGPDNEDYPLFRELWRYHFATGV
  WHQMGTDGYMPRELASMSLVLHGNNLLVFGGTGIPFGESNGNDVHVCNVKYKRWALLSCRGKKPSRIYGQ
  AMAIINGSLYVFGGTTGYIYSTDLHKLDLNTREWTQLKPNNLSCDLPEERYRHEIAHDGQRIYILGGGTS
  WTAYSLNKIHAYNLETNAWEEIATKPHEKIGFPAARRCHSCVQIKNDVFICGGYNGEVILGDIWKLNLQT
  FQWVKLPATMPEPVYFHCAAVTPAGCMYIHGGVVNIHENKRTGSLFKIWLVVPSLLELAWEKLLAAFPNL
  ANLSRTQLLHLGLTQGLIERLK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014997
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014997.4
• RefSeq Size: 6438 bp
• RefSeq ORF: 1329 bp
• Locus ID: 23008
• UniProt ID: Q6PID8
• Cytogenetics: 7q32.2
• Gene Summary: Participates in the oxidative stress-induced cell death through MAP3K5 activation. Inhibits PPP5C phosphatase activity on MAP3K5.[UniProtKB/Swiss-Prot Function]