RFXDC1 (RFX6) (NM_173560) Human Tagged ORF Clone

CAT#: RG206174

RFX6 (tGFP-tagged) - Human regulatory factor X, 6 (RFX6)

Product Images

Circular map for RG206174

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RFXDC1
• Synonyms: dJ955L16.1; MTCHRS; MTFS; RFXDC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206174 representing NM_173560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGTCCCGAAGCTGGAAGACACCTTCCTGCAGGCGCAGCCTGCGCCCCAACTGTCCCCGGGGA
  TCCAGGAAGACTGCTGTGTGCAGCTCCTGGGCAAGGGCTTGCTAGTCTATCCGGAAGAAACAGTGTACCT
  GGCGGCCGAAGGGCAGCCCGGGGGCGAGCAGGGCGGCGGGGAGAAAGGCGAAGACCCGGAGCTGCCGGGG
  GCAGTGAAATCAGAAATGCACTTAAACAATGGTAACTTTTCCTCTGAAGAAGAGGACGCCGACAACCACG
  ACAGCAAAACCAAAGCAGCGGATCAATACCTGTCTCAGAAGAAAACCATCACGCAGATTGTGAAGGATAA
  AAAGAAGCAGACACAGCTCACGCTGCAGTGGCTTGAAGAGAATTACATTGTATGTGAAGGAGTTTGCTTA
  CCACGGTGCATTCTTTATGCACACTACTTAGATTTCTGTAGGAAAGAGAAATTAGAGCCAGCCTGTGCGG
  CCACCTTTGGAAAGACAATTCGCCAGAAGTTTCCCCTCCTAACAACAAGGCGGCTTGGAACAAGAGGCCA
  TTCAAAGTATCATTACTATGGGATTGGCATCAAAGAGAGCAGTGCATATTACCACTCCGTTTATTCTGGA
  AAGGGCTTGACAAGGTTTTCTGGAAGCAAGCTAAAGAATGAGGGTGGCTTCACTCGTAAATATTCGCTTA
  GCTCAAAAACTGGAACACTTCTTCCAGAATTCCCCAGCGCTCAACACCTTGTATACCAAGGATGCATTTC
  TAAGGACAAGGTTGATACGCTCATAATGATGTACAAAACTCACTGCCAGTGTATCCTGGACAATGCAATT
  AATGGAAACTTTGAAGAGATCCAGCATTTTTTATTACACTTTTGGCAAGGAATGCCTGACCATCTCCTTC
  CCCTGCTCGAAAATCCTGTTATCATTGATATTTTCTGTGTTTGTGACTCAATTCTTTATAAGGTTCTTAC
  AGATGTACTCATTCCTGCAACAATGCAAGAAATGCCTGAAAGCTTATTAGCAGACATAAGAAATTTTGCT
  AAAAATTGGGAACAGTGGGTTGTTTCATCCTTGGAAAACTTGCCAGAAGCTCTAACTGACAAGAAAATAC
  CTATTGTGCGAAGATTTGTATCTTCTCTGAAACGACAAACATCTTTCTTACATCTTGCCCAGATTGCCAG
  ACCAGCTCTCTTTGACCAGCATGTCGTTAATTCTATGGTGTCTGATATTGAAAGGGTTGATTTGAACAGC
  ATTGGCTCTCAAGCCCTTCTTACCATTTCAGGCAGCACAGACACTGAATCTGGTATCTACACTGAACATG
  ACTCTATCACTGTGTTCCAAGAACTGAAGGATCTCCTTAAGAAGAATGCCACTGTGGAGGCTTTTATTGA
  ATGGTTGGATACTGTGGTAGAACAGAGAGTTATTAAGACCAGCAAACAAAATGGAAGGTCATTAAAGAAG
  AGAGCTCAAGACTTTCTGTTAAAGTGGAGTTTTTTTGGTGCTCGAGTAATGCATAATCTCACCTTGAACA
  ATGCATCCAGTTTTGGTTCTTTTCATTTGATTCGAATGCTTCTCGATGAATACATTCTCCTGGCCATGGA
  GACCCAGTTTAATAATGACAAAGAGCAGGAGTTACAGAATTTATTGGACAAGTATATGAAGAATTCAGAT
  GCGAGTAAAGCTGCTTTCACTGCTTCTCCGAGTTCATGCTTTCTGGCCAACCGTAATAAAGGGAGCATGG
  TTTCCAGCGACGCTGTGAAGAATGAAAGCCACGTGGAGACAACCTATCTCCCTCTGCCATCCAGTCAACC
  TGGAGGCCTAGGCCCTGCTCTGCACCAGTTCCCTGCTGGGAACACAGACAACATGCCGCTCACAGGTCAA
  ATGGAGCTTTCACAGATTGCTGGTCATCTGATGACACCACCCATTTCTCCAGCCATGGCAAGCCGAGGAA
  GTGTCATTAACCAAGGACCAATGGCAGGGAGGCCCCCAAGTGTGGGCCCAGTACTGTCAGCTCCATCACA
  CTGCTCCACATACCCAGAGCCCATTTATCCCGCTCTCCCTCAAGCCAATCATGACTTTTATAGCACCAGC
  TCTAACTACCAGACTGTGTTTAGGGCACAGCCCCACTCCACATCAGGACTCTATCCTCATCACACCGAGC
  ATGGTCGATGCATGGCTTGGACTGAACAGCAGCTTTCAAGAGACTTCTTCAGTGGCAGCTGTGCGGGGTC
  TCCATATAACTCCCGGCCACCGTCTAGCTATGGCCCATCCCTGCAAGCCCAGGATTCACACAATATGCAG
  TTTTTAAATACAGGAAGCTTCAATTTCTTGAGCAACACAGGAGCTGCCAGCTGCCAAGGAGCAACACTGC
  CTCCTAATTCACCAAATGGATACTATGGAAGCAACATAAACTACCCAGAGTCTCACAGGCTCGGATCAAT
  GGTGAATCAGCACGTTTCTGTCATCAGCAGCATTCGTTCACTGCCCCCCTACAGTGACATCCACGATCCA
  CTTAACATTTTAGATGACAGTGGTAGAAAACAGACCAGCTCGTTTTACACAGACACATCATCTCCAGTTG
  CATGTCGAACTCCAGTCCTAGCTTCCAGTTTGCAAACCCCAATTCCTTCTTCCTCATCCCAATGTATGTA
  TGGAACTTCCAACCAGTATCCAGCTCAAGAAACCCTGGACTCCCATGGAACAAGCAGTAGAGAAATGGTG
  TCCTCTTTACCACCTATCAACACTGTGTTCATGGGAACAGCAGCTGGAGGCACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206174 representing NM_173560
  Red=Cloning site Green=Tags(s)
  
  MAKVPKLEDTFLQAQPAPQLSPGIQEDCCVQLLGKGLLVYPEETVYLAAEGQPGGEQGGGEKGEDPELPG
  AVKSEMHLNNGNFSSEEEDADNHDSKTKAADQYLSQKKTITQIVKDKKKQTQLTLQWLEENYIVCEGVCL
  PRCILYAHYLDFCRKEKLEPACAATFGKTIRQKFPLLTTRRLGTRGHSKYHYYGIGIKESSAYYHSVYSG
  KGLTRFSGSKLKNEGGFTRKYSLSSKTGTLLPEFPSAQHLVYQGCISKDKVDTLIMMYKTHCQCILDNAI
  NGNFEEIQHFLLHFWQGMPDHLLPLLENPVIIDIFCVCDSILYKVLTDVLIPATMQEMPESLLADIRNFA
  KNWEQWVVSSLENLPEALTDKKIPIVRRFVSSLKRQTSFLHLAQIARPALFDQHVVNSMVSDIERVDLNS
  IGSQALLTISGSTDTESGIYTEHDSITVFQELKDLLKKNATVEAFIEWLDTVVEQRVIKTSKQNGRSLKK
  RAQDFLLKWSFFGARVMHNLTLNNASSFGSFHLIRMLLDEYILLAMETQFNNDKEQELQNLLDKYMKNSD
  ASKAAFTASPSSCFLANRNKGSMVSSDAVKNESHVETTYLPLPSSQPGGLGPALHQFPAGNTDNMPLTGQ
  MELSQIAGHLMTPPISPAMASRGSVINQGPMAGRPPSVGPVLSAPSHCSTYPEPIYPALPQANHDFYSTS
  SNYQTVFRAQPHSTSGLYPHHTEHGRCMAWTEQQLSRDFFSGSCAGSPYNSRPPSSYGPSLQAQDSHNMQ
  FLNTGSFNFLSNTGAASCQGATLPPNSPNGYYGSNINYPESHRLGSMVNQHVSVISSIRSLPPYSDIHDP
  LNILDDSGRKQTSSFYTDTSSPVACRTPVLASSLQTPIPSSSSQCMYGTSNQYPAQETLDSHGTSSREMV
  SSLPPINTVFMGTAAGGT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173560
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173560.1, NP_775831.1
• RefSeq Size: 3538 bp
• RefSeq ORF: 2787 bp
• Locus ID: 222546
• UniProt ID: Q8HWS3
• Cytogenetics: 6q22.1
• Protein Families: Transcription Factors
• Gene Summary: The nuclear protein encoded by this gene is a member of the regulatory factor X (RFX) family of transcription factors. Studies in mice suggest that this gene is specifically required for the differentiation of islet cells for the production of insulin, but not for the differentiation of pancreatic polypeptide-producing cells. It regulates the transcription factors involved in beta-cell maturation and function, thus, restricting the expression of the beta-cell differentiation and specification genes. Mutations in this gene are associated with Mitchell-Riley syndrome, which is characterized by neonatal diabetes with pancreatic hypoplasia, duodenal and jejunal atresia, and gall bladder agenesis.[provided by RefSeq, Sep 2010]