GGN (NM_152657) Human Tagged ORF Clone

CAT#: RG206149

GGN (tGFP-tagged) - Human gametogenetin (GGN)

Product Images

Circular map for RG206149

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GGN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206149 representing NM_152657
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACTTGCAGTCGGAGCCATCCGCGGGCGGGGGCTCCCGAAAAGTGCAGCCCTCGGACCGCGCCC
  CCGACTCCCGCCGGACGTCCCTGGTGGAGCCCGAGATGACCTCCCAGGCCATGCGCCTGACTCGTGGGCT
  GGGTGTCTGGTTCCCTGGCAGCGCCACACCCCCGGGACTCATGGTACCCCGGGAGCCCCAGGCCTCACCC
  TCGACCCTGCCCCTCACCTTAGAACGGCCCTCTCCAGTGATGCCCCCTCCTGAAGAGGCGGCCGCGGTCT
  CTGCACCACCCCCGGCCCCCGCGGGGACTCTGCTGCCCGGCCCGTCTAAATGGCAAAAGCCCGCGGGCAC
  TCCAGTTCCCCGGATCCGCCGCCTGCTGGAGGCGAGCCATCGCGGCCAGGGCGACCCTCCGAGCCTCCGC
  CCGCTGAAGCCGCCGCCGCCGCCCCGGCAACTATCCGTGAAGGACACTGTCCCGAGGGCCCCATCCCAAT
  TTCCGCCGCCCCTGGAGACTTGGAAGCCGCCACCACCATTACCTTCTGAACGGCAGCCGGCGGACCGCAG
  AATCACTCCTGCTCTGGCCACACCCGCCTCACCCCCGACAGAAAGCCAGGCTGGGCCCCGCAACCAGGGC
  CAGACGGCCGGCAGGGCTCGCGGAGGGGCGCCTCCCCATGCGGGCGAAGGCGAAATGGCCCAGCCTGCGG
  ATTCCGAGTCCGGTCTGAGCCTGCTGTGTAAAATCACCTTCAAGTCGAGGCCCTCTTTGGCCCCTCCGGC
  AGCCTCGAGTTCCTTAGCAGCCAAAGCTTCGCTGGGGGGCGGCGGAGGCGGCGGCCTCTTTGCTGCCTCA
  GGTGCCATCTCTTACGCCGAGGTCCTGAAGCAAGGGCCCCTGCCTCCTGGAGCCGCTCGCCCCTTGGGAG
  AGGTTTCTCGAGGGGCACAGGAAGCCGAGGGAGGTGATGGAGACGGCGAAGGGTGCTCTGGTCCTCCCTC
  GGCGCCTGCGTCCCAAGCCCGGGCCCTACCGCCGCCACCCTACACCACCTTCCCAGGCTCGAAGCCCAAA
  TTCGACTGGGTTAGCGCTCCCGACGGCCCTGAACGCCACTTCCGCTTCAACGGGGCTGGCGGAGGCATCG
  GGGCGCCGCGACGGCGTGCGGCCGCACTCTCTGGGCCTTGGGGCTCCCCTCCGCCACCACCAGAGCAGAT
  ACACTCAGCTCCCGGGCCCCGGAGGCCCGCACCTGCCCTGCTGGCGCCGCCTACGTTCATCTTCCCAGCA
  CCCACCAATGGCGAGCCCATGCGCCCGGGGCCTCCAGGCCTGCAGGAGTTACCACCGCTGCCACCGCCCA
  CACCGCCGCCCACACTGCAGCCACCAGCGCTCCAGCCAACGCCGCTGCCGGTGGCTCCCCCGCTCACCCC
  GGGTCTGGGCCACAAGGAGTCAGCCCTGGCTCCCACCGCAGCCCCTGCTCTGCCCCCAGCCTTAGCCGCC
  GACCAGGCCCCGGCCCCATCCCCGGCTCCAGCTCCCACCGTGGCTGAGCCCTCGCCGCCTGTGTCCGCGC
  CCGCACCCGCGGCTGCGCCCATCAAGACCCGCACACGCAGGAACAAGGGTTCCCGTGCAGCCCGGGGCGC
  GACCCGTAAGGATGGCTTGCATGGAGATGGTCCTCGCGAACGAGCTACAGCTACCGTGCCTGACAGCAGT
  GGTGGAGGGGGTGGTGGCAGCGGGGCCTCTCAGACTGGGGCAGCTAACACCCGCGCTGCGCGCCACTGGC
  TGCCCTTCCAGGTGCTTAACTCCTGTCCCTGCAAGTGTTACTGCCACCACCAGCCACGCCATCGCCGCCT
  GCCACGCAACGTCTCTGCCTGGCTGAGCACATCCACCAACCACCTGGGCGAGCCACCCTGGGTTGCCACC
  ATCAAGCTGTCCGGCTCTCTGGTGGCCAAGCTGGAGCACTACGACTTGCAGGCCACCCATTCCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206149 representing NM_152657
  Red=Cloning site Green=Tags(s)
  
  MGNLQSEPSAGGGSRKVQPSDRAPDSRRTSLVEPEMTSQAMRLTRGLGVWFPGSATPPGLMVPREPQASP
  STLPLTLERPSPVMPPPEEAAAVSAPPPAPAGTLLPGPSKWQKPAGTPVPRIRRLLEASHRGQGDPPSLR
  PLKPPPPPRQLSVKDTVPRAPSQFPPPLETWKPPPPLPSERQPADRRITPALATPASPPTESQAGPRNQG
  QTAGRARGGAPPHAGEGEMAQPADSESGLSLLCKITFKSRPSLAPPAASSSLAAKASLGGGGGGGLFAAS
  GAISYAEVLKQGPLPPGAARPLGEVSRGAQEAEGGDGDGEGCSGPPSAPASQARALPPPPYTTFPGSKPK
  FDWVSAPDGPERHFRFNGAGGGIGAPRRRAAALSGPWGSPPPPPEQIHSAPGPRRPAPALLAPPTFIFPA
  PTNGEPMRPGPPGLQELPPLPPPTPPPTLQPPALQPTPLPVAPPLTPGLGHKESALAPTAAPALPPALAA
  DQAPAPSPAPAPTVAEPSPPVSAPAPAAAPIKTRTRRNKGSRAARGATRKDGLHGDGPRERATATVPDSS
  GGGGGGSGASQTGAANTRAARHWLPFQVLNSCPCKCYCHHQPRHRRLPRNVSAWLSTSTNHLGEPPWVAT
  IKLSGSLVAKLEHYDLQATHSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152657
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152657.3, NP_689870.3
• RefSeq Size: 2168 bp
• RefSeq ORF: 1959 bp
• Locus ID: 199720
• UniProt ID: Q86UU5
• Cytogenetics: 19q13.2
• Gene Summary: This gene is a germ cell-specific gene that encodes proteins that interact with POG (proliferation of germ cells). Alternatively spliced transcript variants of a similar mouse gene encode at least three different proteins, namely gametogenetin protein 1a, gametogenetin protein 2, and gametogenetin protein 3, which show a perinuclear, cytoplasmic, and nucleolar localization, respectively. These proteins regulate the localization of POG and may play a role in spermatogenesis. [provided by RefSeq, Jul 2008]