DIPK2A (NM_173552) Human Tagged ORF Clone

CAT#: RG206148

C3orf58 (tGFP-tagged) - Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1

Product Images

Circular map for RG206148

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIPK2A
• Synonyms: C3orf58; DIA1; GoPro49; HASF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206148 representing NM_173552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGCCTGGTGCCCCCGAAGCTGGGCCGCCTGTCCCGCTCGCTGAAGCTGGCGGCGCTGGGCAGCC
  TGTTGGTGCTGATGGTGCTGCACTCGCCGTCGCTGCTCGCCTCTTGGCAGCGCAACGAACTGACCGACCG
  GCGCTTCCTGCAGCTCAATAAGTGCCCGGCGTGCTTCGGCACGAGCTGGTGCCGCCGCTTCCTCAACGGG
  CAGGTGGTATTCGAGGCGTGGGGCCGCTTGCGCCTGCTGGACTTCCTCAACGTGAAGAACGTGTACTTCG
  CGCAGTACGGCGAGCCCCGCGAGGGCGGCCGCCGCCGAGTGGTGCTCAAGCGCCTCGGCTCGCAGCGCGA
  GCTGGCGCAGCTCGACCAGAGCATCTGCAAGCGGGCCACCGGCCGGCCCCGCTGCGACCTGCTGCAGGCC
  ATGCCCCGGACCGAGTTCGCGCGCCTCAACGGCGACGTGCGTCTGCTCACGCCCGAGGCGGTGGAGGGCT
  GGTCGGACCTGGTGCACTGCCCCTCGCAGCGCCTTCTCGACCGCCTGGTGCGCCGCTACGCGGAGACCAA
  GGACTCGGGCAGCTTCCTGCTTCGCAACCTCAAGGACTCGGAGCGCATGCAGCTGCTGCTGACCCTGGCC
  TTCAACCCCGAGCCGCTGGTGCTACAGAGTTTTCCGTCTGATGAAGGTTGGCCATTTGCAAAGTATCTTG
  GAGCTTGTGGAAGAATGGTGGCTGTAAATTATGTTGGAGAAGAACTGTGGAGTTACTTTAATGCGCCATG
  GGAAAAACGAGTTGACCTCGCTTGGCAATTAATGGAAATAGCAGAACAGCTTACAAACAATGACTTTGAA
  TTTGCACTCTACCTCCTGGACGTCAGCTTTGACAATTTTGCAGTTGGTCCTAGAGATGGGAAGGTAATCA
  TTGTGGATGCTGAAAATGTTTTGGTTGCTGACAAAAGATTAATTAGACAAAATAAACCTGAAAATTGGGA
  TGTATGGTATGAAAGCAAGTTTGATGACTGTGATAAGGAGGCTTGCTTATCATTTTCAAAAGAAATTCTT
  TGTGCTCGTGCCACTGTGGACCACAATTACTATGCTGTTTGTCAGAACCTCTTATCCAGACATGCCACCT
  GGCGTGGCACTTCTGGAGGACTCCTTCATGATCCACCAAGTGAAATTGCCAAAGATGGCCGGCTCGAGGC
  CTTGCTGGATGAGTGTGCCAACCCAAAGAAGCGCTATGGCAGATTCCAGGCTGCAAAAGAACTGCGTGAA
  TACCTAGCACAATTAAGTAACAACGTGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206148 representing NM_173552
  Red=Cloning site Green=Tags(s)
  
  MWRLVPPKLGRLSRSLKLAALGSLLVLMVLHSPSLLASWQRNELTDRRFLQLNKCPACFGTSWCRRFLNG
  QVVFEAWGRLRLLDFLNVKNVYFAQYGEPREGGRRRVVLKRLGSQRELAQLDQSICKRATGRPRCDLLQA
  MPRTEFARLNGDVRLLTPEAVEGWSDLVHCPSQRLLDRLVRRYAETKDSGSFLLRNLKDSERMQLLLTLA
  FNPEPLVLQSFPSDEGWPFAKYLGACGRMVAVNYVGEELWSYFNAPWEKRVDLAWQLMEIAEQLTNNDFE
  FALYLLDVSFDNFAVGPRDGKVIIVDAENVLVADKRLIRQNKPENWDVWYESKFDDCDKEACLSFSKEIL
  CARATVDHNYYAVCQNLLSRHATWRGTSGGLLHDPPSEIAKDGRLEALLDECANPKKRYGRFQAAKELRE
  YLAQLSNNVR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173552
• ORF Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173552.5
• RefSeq Size: 4370 bp
• RefSeq ORF: 1293 bp
• Locus ID: 205428
• UniProt ID: Q8NDZ4
• Cytogenetics: 3q24
• Gene Summary: May play a role in cardiomyocyte proliferation through paracrine signaling and activation of the PPI3K-AKT-CDK7 signaling cascade.[UniProtKB/Swiss-Prot Function]