DIPK2A (NM_173552) Human Tagged ORF Clone

  • TrueORF®
SKU
RG206148
C3orf58 (tGFP-tagged) - Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1
  $932.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DIPK2A
Synonyms C3orf58; DIA1; GoPro49; HASF
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206148 representing NM_173552
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCGCCTGGTGCCCCCGAAGCTGGGCCGCCTGTCCCGCTCGCTGAAGCTGGCGGCGCTGGGCAGCC
TGTTGGTGCTGATGGTGCTGCACTCGCCGTCGCTGCTCGCCTCTTGGCAGCGCAACGAACTGACCGACCG
GCGCTTCCTGCAGCTCAATAAGTGCCCGGCGTGCTTCGGCACGAGCTGGTGCCGCCGCTTCCTCAACGGG
CAGGTGGTATTCGAGGCGTGGGGCCGCTTGCGCCTGCTGGACTTCCTCAACGTGAAGAACGTGTACTTCG
CGCAGTACGGCGAGCCCCGCGAGGGCGGCCGCCGCCGAGTGGTGCTCAAGCGCCTCGGCTCGCAGCGCGA
GCTGGCGCAGCTCGACCAGAGCATCTGCAAGCGGGCCACCGGCCGGCCCCGCTGCGACCTGCTGCAGGCC
ATGCCCCGGACCGAGTTCGCGCGCCTCAACGGCGACGTGCGTCTGCTCACGCCCGAGGCGGTGGAGGGCT
GGTCGGACCTGGTGCACTGCCCCTCGCAGCGCCTTCTCGACCGCCTGGTGCGCCGCTACGCGGAGACCAA
GGACTCGGGCAGCTTCCTGCTTCGCAACCTCAAGGACTCGGAGCGCATGCAGCTGCTGCTGACCCTGGCC
TTCAACCCCGAGCCGCTGGTGCTACAGAGTTTTCCGTCTGATGAAGGTTGGCCATTTGCAAAGTATCTTG
GAGCTTGTGGAAGAATGGTGGCTGTAAATTATGTTGGAGAAGAACTGTGGAGTTACTTTAATGCGCCATG
GGAAAAACGAGTTGACCTCGCTTGGCAATTAATGGAAATAGCAGAACAGCTTACAAACAATGACTTTGAA
TTTGCACTCTACCTCCTGGACGTCAGCTTTGACAATTTTGCAGTTGGTCCTAGAGATGGGAAGGTAATCA
TTGTGGATGCTGAAAATGTTTTGGTTGCTGACAAAAGATTAATTAGACAAAATAAACCTGAAAATTGGGA
TGTATGGTATGAAAGCAAGTTTGATGACTGTGATAAGGAGGCTTGCTTATCATTTTCAAAAGAAATTCTT
TGTGCTCGTGCCACTGTGGACCACAATTACTATGCTGTTTGTCAGAACCTCTTATCCAGACATGCCACCT
GGCGTGGCACTTCTGGAGGACTCCTTCATGATCCACCAAGTGAAATTGCCAAAGATGGCCGGCTCGAGGC
CTTGCTGGATGAGTGTGCCAACCCAAAGAAGCGCTATGGCAGATTCCAGGCTGCAAAAGAACTGCGTGAA
TACCTAGCACAATTAAGTAACAACGTGAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206148 representing NM_173552
Red=Cloning site Green=Tags(s)

MWRLVPPKLGRLSRSLKLAALGSLLVLMVLHSPSLLASWQRNELTDRRFLQLNKCPACFGTSWCRRFLNG
QVVFEAWGRLRLLDFLNVKNVYFAQYGEPREGGRRRVVLKRLGSQRELAQLDQSICKRATGRPRCDLLQA
MPRTEFARLNGDVRLLTPEAVEGWSDLVHCPSQRLLDRLVRRYAETKDSGSFLLRNLKDSERMQLLLTLA
FNPEPLVLQSFPSDEGWPFAKYLGACGRMVAVNYVGEELWSYFNAPWEKRVDLAWQLMEIAEQLTNNDFE
FALYLLDVSFDNFAVGPRDGKVIIVDAENVLVADKRLIRQNKPENWDVWYESKFDDCDKEACLSFSKEIL
CARATVDHNYYAVCQNLLSRHATWRGTSGGLLHDPPSEIAKDGRLEALLDECANPKKRYGRFQAAKELRE
YLAQLSNNVR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_173552
ORF Size 1290 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173552.5
RefSeq Size 4370 bp
RefSeq ORF 1293 bp
Locus ID 205428
UniProt ID Q8NDZ4
Cytogenetics 3q24
Summary May play a role in cardiomyocyte proliferation through paracrine signaling and activation of the PPI3K-AKT-CDK7 signaling cascade.[UniProtKB/Swiss-Prot Function]
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Documents
Resources
"NM_173552" in other vectors (4)
SKU Description Size Price
RC206148 C3orf58 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1 10 ug
$732.00
RC206148L3 Lenti-ORF clone of C3orf58 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1 10 ug
$1,032.00
RC206148L4 Lenti-ORF clone of C3orf58 (mGFP-tagged)-Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1 10 ug
$1,032.00
SC101127 C3orf58 (untagged)-Human chromosome 3 open reading frame 58 (C3orf58), transcript variant 1 10 ug
$686.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.