SLC46A3 (NM_181785) Human Tagged ORF Clone

CAT#: RG206102

SLC46A3 (tGFP-tagged) - Human solute carrier family 46, member 3 (SLC46A3), transcript variant 1

Product Images

Circular map for RG206102

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC46A3
• Synonyms: FKSG16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206102 representing NM_181785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTTTATTTGTAGAACCTGCCATTTTCCTTAGTGCATTTGCTATGACTTTGACCGGTCCACTGA
  CAACGCAATATGTTTATCGGAGAATATGGGAAGAAACTGGCAACTACACTTTTTCATCTGATAGCAATAT
  TTCTGAGTGTGAAAAAAACAAAAGCAGCCCAATTTTTGCATTCCAGGAGGAAGTTCAGAAAAAAGTGTCA
  CGTTTTAATCTGCAGATGGACATAAGTGGATTAATTCCTGGTCTAGTGTCTACATTCATACTTTTGTCTA
  TTAGTGATCACTACGGACGAAAATTCCCTATGATTTTGTCTTCCGTTGGTGCTCTTGCAACCAGCGTTTG
  GCTCTGTTTGCTTTGCTATTTTGCCTTTCCATTCCAGCTTTTGATTGCATCTACCTTCATTGGTGCATTT
  TGTGGCAATTATACCACATTTTGGGGAGCTTGCTTTGCCTATATAGTTGATCAGTTTAAAGAACACAAAC
  AAAAAACAATTCGAATAGCTATCATTGACTTTCTACTTGGACTTGTTACTGGACTAACAGGACTGTCATC
  TGGCTATTTTATTAGAGAGCTAGGTTTTGAGTGGTCGTTTCTAATTATTGCTGTGTCTCTTGCTGTTAAT
  TTGATCTATATTTTATTTTTTCTCGGAGATCCAGTGAAAGAGTGTTCATCTCAGAATGTTACTATGTCAT
  GTAGTGAAGGCTTCAAAAACCTATTTTACCGAACTTACATGCTTTTTAAGAATGCTTCTGGTAAGAGACG
  ATTTTTGCTCTGTTTGTTACTTTTTACAGTAATCACTTATTTTTTTGTGGTAATTGGCATTGCCCCAATT
  TTTATCCTTTATGAATTGGATTCACCACTCTGCTGGAATGAAGTTTTTATAGGTTATGGATCAGCTTTGG
  GTAGTGCCTCTTTTTTGACTAGTTTCCTAGGAATATGGCTTTTTTCTTATTGTATGGAAGATATTCATAT
  GGCCTTCATTGGGATTTTTACCACGATGACAGGAATGGCTATGACCGCGTTTGCCAGTACAACACTGATG
  ATGTTTTTAGCCAGGGTGCCGTTCCTTTTCACTATTGTGCCATTCTCTGTTCTACGGTCCATGTTGTCAA
  AAGTGGTTCGTTCGACTGAACAAGGTACCCTGTTTGCTTGTATTGCTTTCTTAGAAACACTTGGAGGAGT
  CACTGCAGTTTCTACTTTTAATGGAATTTACTCAGCCACTGTTGCTTGGTACCCTGGCTTCACTTTCCTG
  CTGTCTGCTGGTCTGTTACTACTTCCAGCCATCAGTCTATGTGTTGTCAAGTGTACCAGCTGGAATGAGG
  GAAGCTATGAACTTCTTATACAAGAAGAATCCAGTGAAGATGCTTCAGACAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206102 representing NM_181785
  Red=Cloning site Green=Tags(s)
  
  MKILFVEPAIFLSAFAMTLTGPLTTQYVYRRIWEETGNYTFSSDSNISECEKNKSSPIFAFQEEVQKKVS
  RFNLQMDISGLIPGLVSTFILLSISDHYGRKFPMILSSVGALATSVWLCLLCYFAFPFQLLIASTFIGAF
  CGNYTTFWGACFAYIVDQFKEHKQKTIRIAIIDFLLGLVTGLTGLSSGYFIRELGFEWSFLIIAVSLAVN
  LIYILFFLGDPVKECSSQNVTMSCSEGFKNLFYRTYMLFKNASGKRRFLLCLLLFTVITYFFVVIGIAPI
  FILYELDSPLCWNEVFIGYGSALGSASFLTSFLGIWLFSYCMEDIHMAFIGIFTTMTGMAMTAFASTTLM
  MFLARVPFLFTIVPFSVLRSMLSKVVRSTEQGTLFACIAFLETLGGVTAVSTFNGIYSATVAWYPGFTFL
  LSAGLLLLPAISLCVVKCTSWNEGSYELLIQEESSEDASDR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181785
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181785.1, NP_861450.1
• RefSeq Size: 3176 bp
• RefSeq ORF: 1386 bp
• Locus ID: 283537
• UniProt ID: Q7Z3Q1
• Cytogenetics: 13q12.3
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a member of a transmembrane protein family that transports small molecules across membranes. The encoded protein has been found in lysosomal membranes, where it can transport catabolites from the lysosomes to the cytoplasm. This protein has been shown to be an effective transporter of the cytotoxic drug maytansine, which is used in antibody-based targeting of cancer cells. [provided by RefSeq, Dec 2016]