SLC20A2 (NM_006749) Human Tagged ORF Clone

CAT#: RG206029

SLC20A2 (tGFP-tagged) - Human solute carrier family 20 (phosphate transporter), member 2 (SLC20A2)

Product Images

Circular map for RG206029

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC20A2
• Synonyms: GLVR-2; GLVR2; IBGC1; IBGC2; IBGC3; MLVAR; PIT-2; PIT2; Ram-1; RAM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206029 representing NM_006749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGATGAGTATTTGTGGATGGTCATTTTGGGTTTCATCATAGCTTTCATCTTGGCCTTTTCTG
  TTGGTGCAAACGATGTTGCCAACTCCTTTGGTACAGCCGTGGGCTCTGGTGTGGTGACCTTGAGGCAGGC
  ATGCATTTTAGCTTCAATATTTGAAACCACCGGCTCCGTGTTACTAGGCGCCAAAGTAGGAGAAACCATT
  CGCAAAGGTATCATTGACGTGAACCTGTACAACGAGACGGTGGAGACTCTCATGGCTGGGGAAGTTAGTG
  CCATGGTTGGTTCCGCTGTGTGGCAGCTGATTGCTTCCTTCCTGAGGCTTCCAATCTCAGGAACGCACTG
  CATTGTGGGTTCTACTATAGGATTCTCACTGGTCGCAATCGGTACCAAAGGTGTGCAGTGGATGGAGCTT
  GTCAAGATTGTTGCTTCTTGGTTTATATCTCCACTGTTGTCTGGTTTCATGTCTGGCCTGCTGTTTGTAC
  TCATCAGAATTTTCATCTTAAAAAAGGAAGACCCTGTTCCCAATGGCCTCCGGGCACTCCCAGTATTCTA
  TGCTGCTACCATAGCAATCAATGTCTTTTCCATCATGTACACAGGAGCACCAGTGCTCGGCCTTGTTCTC
  CCCATGTGGGCCATAGCCCTCATTTCCTTTGGTGTCGCCCTCCTGTTCGCTTTTTTTGTGTGGCTCTTCG
  TGTGTCCGTGGATGCGGAGGAAAATAACAGGCAAATTACAAAAAGAAGGTGCTTTATCACGAGTATCTGA
  CGAAAGCCTCAGTAAGGTTCAGGAAGCAGAGTCCCCAGTATTTAAAGAGCTACCAGGTGCCAAGGCTAAT
  GATGACAGCACCATCCCGCTCACGGGAGCAGCAGGGGAGACACTGGGGACCTCGGAAGGCACTTCTGCGG
  GCAGCCACCCTCGGGCTGCATACGGAAGAGCACTGTCCATGACCCATGGCTCTGTGAAATCGCCCATCTC
  CAACGGCACCTTCGGCTTCGACGGCCACACCAGGAGCGACGGTCATGTGTACCACACCGTGCACAAAGAC
  TCGGGGCTCTACAAAGATCTGCTGCACAAAATCCACATCGACAGGGGCCCCGAGGAGAAGCCAGCCCAGG
  AAAGCAACTACCGGCTGCTGCGCCGAAACAACAGTTACACCTGCTACACCGCAGCCATTTGTGGGCTGCC
  AGTGCACGCCACCTTTCGAGCTGCGGACTCATCGGCCCCAGAGGACAGTGAGAAGCTGGTGGGCGACACC
  GTGTCCTACTCCAAGAAGAGGCTGCGCTACGACAGCTACTCGAGCTACTGTAACGCGGTGGCAGAGGCGG
  AGATCGAGGCGGAGGAGGGCGGCGTGGAGATGAAGCTGGCGTCGGAGCTGGCCGACCCTGACCAGCCGCG
  AGAGGACCCTGCAGAGGAGGAGAAGGAGGAGAAGGACGCACCCGAGGTTCACCTCCTGTTCCATTTCCTG
  CAGGTCCTCACCGCCTGTTTCGGGTCCTTTGCTCACGGCGGCAATGACGTGAGTAATGCCATCGGTCCCC
  TGGTAGCCTTGTGGCTGATTTACAAACAAGGCGGGGTAACGCAAGAAGCAGCTACACCCGTCTGGCTGCT
  GTTTTATGGAGGAGTTGGAATCTGCACAGGCCTCTGGGTCTGGGGGAGAAGAGTGATCCAGACCATGGGG
  AAGGACCTCACTCCCATCACGCCGTCCAGCGGCTTCACGATCGAGCTGGCCTCAGCCTTCACAGTGGTGA
  TCGCCTCCAACATCGGGCTTCCAGTCAGCACCACGCACTGTAAGGTGGGCTCGGTGGTGGCCGTGGGCTG
  GATCCGCTCCCGCAAGGCTGTGGACTGGCGCCTCTTTCGGAACATCTTCGTGGCCTGGTTCGTGACCGTC
  CCTGTGGCTGGGCTGTTCAGCGCTGCTGTCATGGCTCTTCTCATGTATGGGATCCTTCCATATGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206029 representing NM_006749
  Red=Cloning site Green=Tags(s)
  
  MAMDEYLWMVILGFIIAFILAFSVGANDVANSFGTAVGSGVVTLRQACILASIFETTGSVLLGAKVGETI
  RKGIIDVNLYNETVETLMAGEVSAMVGSAVWQLIASFLRLPISGTHCIVGSTIGFSLVAIGTKGVQWMEL
  VKIVASWFISPLLSGFMSGLLFVLIRIFILKKEDPVPNGLRALPVFYAATIAINVFSIMYTGAPVLGLVL
  PMWAIALISFGVALLFAFFVWLFVCPWMRRKITGKLQKEGALSRVSDESLSKVQEAESPVFKELPGAKAN
  DDSTIPLTGAAGETLGTSEGTSAGSHPRAAYGRALSMTHGSVKSPISNGTFGFDGHTRSDGHVYHTVHKD
  SGLYKDLLHKIHIDRGPEEKPAQESNYRLLRRNNSYTCYTAAICGLPVHATFRAADSSAPEDSEKLVGDT
  VSYSKKRLRYDSYSSYCNAVAEAEIEAEEGGVEMKLASELADPDQPREDPAEEEKEEKDAPEVHLLFHFL
  QVLTACFGSFAHGGNDVSNAIGPLVALWLIYKQGGVTQEAATPVWLLFYGGVGICTGLWVWGRRVIQTMG
  KDLTPITPSSGFTIELASAFTVVIASNIGLPVSTTHCKVGSVVAVGWIRSRKAVDWRLFRNIFVAWFVTV
  PVAGLFSAAVMALLMYGILPYV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006749
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006749.5
• RefSeq Size: 3685 bp
• RefSeq ORF: 1959 bp
• Locus ID: 6575
• UniProt ID: Q08357
• Cytogenetics: 8p11.21
• Domains: PHO4
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the inorganic phosphate transporter family. The encoded protein is a type 3 sodium-dependent phosphate symporter that plays an important role in phosphate homeostasis by mediating cellular phosphate uptake. The encoded protein also confers susceptibility to viral infection as a gamma-retroviral receptor. Mutations in this gene may play a role in familial idiopathic basal ganglia calcification. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]