BIRC5 (NM_001168) Human Tagged ORF Clone
BIRC5 (GFP-tagged) - Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1
|Product Name||BIRC5 (NM_001168) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG205935 representing NM_001168
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG205935 representing NM_001168
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||426 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2655 bp|
|RefSeq ORF||429 bp|
|Protein Families||Druggable Genome, Stem cell - Pluripotency|
|Protein Pathways||Colorectal cancer, Pathways in cancer|
|Gene Summary||This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]|
|The use of this cDNA Clones has been cited in the following citations:|
Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli
,Tsai, SL;Chang, YC;Sarvagalla, S;Wang, S;Coumar, MS;Cheung, CHA;,
Protein Expr. Purif. 2019
,PubMed ID 31004782
YM155 exerts potent cytotoxic activity against quiescent (G0/G1) multiple myeloma and bortezomib resistant cells via inhibition of survivin and Mcl-1
,Ookura, M;Fujii, T;Yagi, H;Ogawa, T;Kishi, S;Hosono, N;Shigemi, H;Yamauchi, T;Ueda, T;Yoshida, A;,
,PubMed ID 29340073
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
,Lin, KY;Cheng, SM;Tsai, SL;Tsai, JY;Lin, CH;Cheung, CH;,
Onco Targets Ther May 2016
,PubMed ID 27217778
INVESTIGATIONS OF YM155, AN INHIBITOR OF SURVIVIN, AS A MOLECULAR MULTI-TARGETED AGENT FOR RENAL CELL CARCINOMA
Thesis Jan 2016
|cDNA Clone Resources|
|SC119405||BIRC5 (untagged)-Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1||
|RC205935||BIRC5 (Myc-DDK-tagged)-Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1||
|RC205935L1||Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, Myc-DDK-tagged||
|RC205935L2||Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, mGFP tagged||
|RC205935L3||Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, Myc-DDK-tagged||
|RC205935L4||Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, mGFP tagged||