FAM126A (NM_032581) Human Tagged ORF Clone

CAT#: RG205807

FAM126A (tGFP-tagged) - Human family with sequence similarity 126, member A (FAM126A)

Product Images

Circular map for RG205807

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FAM126A
• Synonyms: DRCTNNB1A; HCC; HLD5; HYCC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205807 representing NM_032581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTACTTCAGAGAAAGGGGTTGTGGAGGAATGGTTGTCAGAGTTTAAGACATTACCAGAAACATCTT
  TACCAAATTATGCCACAAATTTGAAAGACAAGAGTTCTTTAGTTTCATCTCTCTATAAAGTTATCCAGGA
  GCCACAAAGTGAGTTGCTAGAACCTGTCTGTCACCAGCTCTTTGAATTCTATCGCAGTGGAGAGGAGCAG
  TTGCTTCAATTTACGCTGCAATTTCTCCCAGAACTAATTTGGTGCTACCTTGCAGTCTCAGCCAGCAGAA
  ATGTGCATAGCAGTGGATGCATTGAAGCTCTTCTTCTAGGGGTTTACAATTTGGAAATAGTTGACAAACA
  GGGACATACCAAAGTATTGAGTTTTACGATTCCATCTTTATCCAAACCATCTGTATACCATGAACCTTCC
  AGCATTGGGTCCATGGCTCTGACTGAGAGTGCACTATCCCAGCATGGTTTGTCAAAAGTGGTATACAGTG
  GACCTCATCCTCAAAGGGAGATGCTGACAGCACAGAATCGGTTTGAAGTGTTGACATTCCTTTTGTTGTG
  TTACAATGCTGCCTTAACTTACATGCCCAGTGTTTCTCTTCAGTCACTGTGTCAAATTTGTTCAAGAATC
  TGTGTTTGTGGATATCCTCGACAACATGTAAGAAAATATAAAGGTATAAGTAGCAGGATACCAGTTTCTT
  CAGGATTCATGGTGCAAATGTTAACAGGGATTTATTTTGCCTTTTATAATGGAGAATGGGATCTAGCTCA
  AAAAGCACTGGATGATATTATATACAGAGCCCAGCTAGAATTATATCCAGAGCCATTGCTGGTTGCTAAT
  GCAATAAAGGCTTCATTGCCTCATGGTCCCATGAAATCTAATAAGGAAGGTACAAGGTGTATTCAAGTTG
  AAATCACACCAACTTCCTCTCGAATATCAAGAAATGCAGTAACCAGCATGTCAATAAGGGGTCATAGGTG
  GAAAAGACATGGAAATACAGAATTAACAGGTCAAGAAGAACTGATGGAGATATCTGAAGTTGACGAGGGA
  TTTTATTCCAGGGCTGCGTCTAGTACCAGCCAGTCGGGTTTATCAAACAGTAGTCACAATTGTAGTAACA
  AGCCAAGTATAGGAAAGAACCACAGACGGTCAGGAGGAAGCAAAACTGGAGGAAAAGAAAAAGAAACTAC
  AGGGGAATCTTGCAAAGATCACTTTGCTCGAAAACAAACTCAGAGAGCCCAAAGTGAGAATCTTGAGCTT
  CTCTCTTTGAAGAGACTTACTTTAACAACCAGCCAATCCCTACCTAAGCCTAGTAGCCATGGTTTGGCTA
  AGACCGCAGCGACTGTATTTAGTAAATCCTTTGAACAAGTCAGTGGTGTCACAGTCCCACATAACCCGTC
  ATCTGCTGTTGGTTGTGGGGCTGGGACAGATGCCAATAGGTTTTCCGCTTGTAGTCTCCAAGAAGAAAAG
  CTTATTTACGTTTCAGAAAGAACTGAACTTCCAATGAAGCATCAATCAGGTCAGCAGAGACCTCCTAGTA
  TTAGCATTACTCTGTCCACAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205807 representing NM_032581
  Red=Cloning site Green=Tags(s)
  
  MFTSEKGVVEEWLSEFKTLPETSLPNYATNLKDKSSLVSSLYKVIQEPQSELLEPVCHQLFEFYRSGEEQ
  LLQFTLQFLPELIWCYLAVSASRNVHSSGCIEALLLGVYNLEIVDKQGHTKVLSFTIPSLSKPSVYHEPS
  SIGSMALTESALSQHGLSKVVYSGPHPQREMLTAQNRFEVLTFLLLCYNAALTYMPSVSLQSLCQICSRI
  CVCGYPRQHVRKYKGISSRIPVSSGFMVQMLTGIYFAFYNGEWDLAQKALDDIIYRAQLELYPEPLLVAN
  AIKASLPHGPMKSNKEGTRCIQVEITPTSSRISRNAVTSMSIRGHRWKRHGNTELTGQEELMEISEVDEG
  FYSRAASSTSQSGLSNSSHNCSNKPSIGKNHRRSGGSKTGGKEKETTGESCKDHFARKQTQRAQSENLEL
  LSLKRLTLTTSQSLPKPSSHGLAKTAATVFSKSFEQVSGVTVPHNPSSAVGCGAGTDANRFSACSLQEEK
  LIYVSERTELPMKHQSGQQRPPSISITLSTD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032581
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032581.4
• RefSeq Size: 3768 bp
• RefSeq ORF: 1566 bp
• Locus ID: 84668
• UniProt ID: Q9BYI3
• Cytogenetics: 7p15.3
• Gene Summary: The protein encoded by this gene may play a part in the beta-catenin/Lef signaling pathway. Expression of this gene is down-regulated by beta-catenin. Defects in this gene are a cause of hypomyelination with congenital cataract (HCC). [provided by RefSeq, Oct 2008]