STAT5A (NM_003152) Human Tagged ORF Clone
CAT#: RG205753
- TrueORF®
STAT5A (tGFP-tagged) - Human signal transducer and activator of transcription 5A (STAT5A)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_003152" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | STAT5A |
Synonyms | MGF; STAT5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205753 representing NM_003152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCTGGATCCAGGCCCAGCAGCTGCAGGGAGACGCGCTGCGCCAGATGCAGGTGCTGTACGGCC AGCACTTCCCCATCGAGGTCCGGCACTACTTGGCCCAGTGGATTGAGAGCCAGCCATGGGATGCCATTGA CTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCCTGGAGGGCCTGGTGCAGGAGCTGCAGAAG AAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAAGCTGGGGCACTACGCCACGCAGC TCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTCCGCTGCATCCGGCACATTCTGTACAATGAACA GAGGCTGGTCCGAGAAGCCAACAATTGCAGCTCTCCGGCTGGGATCCTGGTTGACGCCATGTCCCAGAAG CACCTTCAGATCAACCAGACATTTGAGGAGCTGCGACTGGTCACGCAGGACACAGAGAATGAGCTGAAGA AACTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGCCTGAGGATCCAAGCTCAGTTTGC CCAGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGGGAGACGGCCCTCCAGCAGAAGCAGGTGTCT CTGGAGGCCTGGTTGCAGCGTGAGGCACAGACACTGCAGCAGTACCGCGTGGAGCTGGCCGAGAAGCACC AGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGATGACGAGCTGATCCAGTGGAAGCG GCGGCAGCAGCTGGCCGGGAACGGCGGGCCCCCCGAGGGCAGCCTGGACGTGCTACAGTCCTGGTGTGAG AAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACCTCTGCCAGCAGCTGC CCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAACGCCACCATCACGGACATTATCTCAGCCCT GGTGACCAGCACATTCATCATTGAGAAGCAGCCTCCTCAGGTCCTGAAGACCCAGACCAAGTTTGCAGCC ACCGTACGCCTGCTGGTGGGCGGGAAGCTGAACGTGCACATGAATCCCCCCCAGGTGAAGGCCACCATCA TCAGTGAGCAGCAGGCCAAGTCTCTGCTTAAAAATGAGAACACCCGCAACGAGTGCAGTGGTGAGATCCT GAACAACTGCTGCGTGATGGAGTACCACCAAGCCACGGGCACCCTCAGTGCCCACTTCAGGAACATGTCA CTGAAGAGGATCAAGCGTGCTGACCGGCGGGGTGCAGAGTCCGTGACAGAGGAGAAGTTCACAGTCCTGT TTGAGTCTCAGTTCAGTGTTGGCAGCAATGAGCTTGTGTTCCAGGTGAAGACTCTGTCCCTACCTGTGGT TGTCATCGTCCACGGCAGCCAGGACCACAATGCCACGGCTACTGTGCTGTGGGACAATGCCTTTGCTGAG CCGGGCAGGGTGCCATTTGCCGTGCCTGACAAAGTGCTGTGGCCGCAGCTGTGTGAGGCGCTCAACATGA AATTCAAGGCCGAAGTGCAGAGCAACCGGGGCCTGACCAAGGAGAACCTCGTGTTCCTGGCGCAGAAACT GTTCAACAACAGCAGCAGCCACCTGGAGGACTACAGTGGCCTGTCCGTGTCCTGGTCCCAGTTCAACAGG GAGAACTTGCCGGGCTGGAACTACACCTTCTGGCAGTGGTTTGACGGGGTGATGGAGGTGTTGAAGAAGC ACCACAAGCCCCACTGGAATGATGGGGCCATCCTAGGTTTTGTGAATAAGCAACAGGCCCACGACCTGCT CATCAACAAGCCCGACGGGACCTTCTTGTTGCGCTTTAGTGACTCAGAAATCGGGGGCATCACCATCGCC TGGAAGTTTGATTCCCCGGAACGCAACCTGTGGAACCTGAAACCATTCACCACGCGGGATTTCTCCATCA GGTCCCTGGCTGACCGGCTGGGGGACCTGAGCTATCTCATCTATGTGTTTCCTGACCGCCCCAAGGATGA GGTCTTCTCCAAGTACTACACTCCTGTGCTGGCTAAAGCTGTTGATGGATATGTGAAACCACAGATCAAG CAAGTGGTCCCTGAGTTTGTGAATGCATCTGCAGATGCTGGGGGCAGCAGCGCCACGTACATGGACCAGG CCCCCTCCCCAGCTGTGTGCCCCCAGGCTCCCTATAACATGTACCCACAGAACCCTGACCATGTACTCGA TCAGGATGGAGAATTCGACCTGGATGAGACCATGGATGTGGCCAGGCACGTGGAGGAACTCTTACGCCGA CCAATGGACAGTCTTGACTCCCGCCTCTCGCCCCCTGCCGGTCTTTTCACCTCTGCCAGAGGCTCCCTCT CA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205753 representing NM_003152
Red=Cloning site Green=Tags(s) MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQK KAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQK HLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVS LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCE KLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMS LKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAE PGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNR ENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIA WKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIK QVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRR PMDSLDSRLSPPAGLFTSARGSLS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003152 |
ORF Size | 2382 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003152.2, NP_003143.2 |
RefSeq Size | 4298 bp |
RefSeq ORF | 2385 bp |
Locus ID | 6776 |
UniProt ID | P42229 |
Cytogenetics | 17q21.2 |
Domains | SH2, STAT |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
Protein Pathways | Acute myeloid leukemia, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer |
Gene Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205753 | STAT5A (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,090.00 |
|
RC205753L1 | Lenti-ORF clone of STAT5A (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,390.00 |
|
RC205753L2 | Lenti-ORF clone of STAT5A (mGFP-tagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,390.00 |
|
RC205753L3 | Lenti-ORF clone of STAT5A (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,390.00 |
|
RC205753L4 | Lenti-ORF clone of STAT5A (mGFP-tagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,390.00 |
|
SC118194 | STAT5A (untagged)-Human signal transducer and activator of transcription 5A (STAT5A) |
USD 1,845.00 |
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