ZNF444 (NM_018337) Human Tagged ORF Clone

CAT#: RG205661

ZNF444 (tGFP-tagged) - Human zinc finger protein 444 (ZNF444)

Product Images

Circular map for RG205661

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF444
• Synonyms: EZF-2; EZF2; ZSCAN17
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205661 representing NM_018337
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGCGGTGCCCGTGAAGCAGGAGGCCGAGGGCCTGGCGCTGGACTCCCCGTGGCACCGCTTCC
  GCCGCTTCCACCTGGGCGACGCGCCGGGCCCGCGGGAGGCGCTGGGGCTGCTCCGCGCCCTGTGCCGGGA
  CTGGCTGCGGCCCGAGGTGCACACCAAGGAGCAGATGTTGGAGCTGCTGGTGCTGGAACAGTTCCTGAGC
  GCGCTGCCCGCCGACACGCAGGCCTGGGTGTGCAGCCGGCAGCCGCAGAGCGGGGAGGAGGCGGTGGCCC
  TGCTGGAGGAGCTCTGGGGGCCAGCAGCCTCCCCCGATGGGTCGTCAGCAACGAGGGTGCCTCAGGATGT
  GACGCAGGGCCCTGGGGCCACAGGTGGAAAGGAGGACAGTGGGATGATTCCCTTAGGCACCGCCCCTGGG
  GCTGAGGGGCCGGCGCCTGGGGACTCCCAGGCTGTGCGCCCCTACAAGCAGGAGCCCAGCAGCCCCCCGC
  TGGCGCCTGGCCTGCCCGCCTTCCTAGCGGCCCCGGGCACCACGTCCTGCCCCGAGTGCGGCAAAACGTC
  CCTGAAACCAGCTCACCTGCTGCGCCACCGGCAGAGCCACTCGGGCGAGAAGCCGCACGCCTGCCCTGAG
  TGCGGGAAGGCCTTTCGGCGCAAGGAGCACCTGCGGCGCCACCGCGACACGCACCCCGGCAGCCCCGGCA
  GCCCCGGGCCCGCGCTGCGCCCTCTGCCCGCCCGTGAGAAGCCCCACGCGTGCTGCGAGTGTGGCAAGAC
  CTTCTACTGGCGCGAGCACCTGGTGCGCCACCGCAAGACGCACTCGGGAGCGCGGCCCTTTGCCTGCTGG
  GAGTGTGGCAAGGGCTTCGGGCGCCGCGAGCACGTGCTGCGCCACCAGCGCATCCACGGCCGGGCAGCGG
  CCAGCGCGCAGGGGGCGGTAGCTCCGGGCCCGGATGGTGGAGGCCCCTTCCCGCCCTGGCCCTTGGGT
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205661 representing NM_018337
  Red=Cloning site Green=Tags(s)
  
  MEVAVPVKQEAEGLALDSPWHRFRRFHLGDAPGPREALGLLRALCRDWLRPEVHTKEQMLELLVLEQFLS
  ALPADTQAWVCSRQPQSGEEAVALLEELWGPAASPDGSSATRVPQDVTQGPGATGGKEDSGMIPLGTAPG
  AEGPAPGDSQAVRPYKQEPSSPPLAPGLPAFLAAPGTTSCPECGKTSLKPAHLLRHRQSHSGEKPHACPE
  CGKAFRRKEHLRRHRDTHPGSPGSPGPALRPLPAREKPHACCECGKTFYWREHLVRHRKTHSGARPFACW
  ECGKGFGRREHVLRHQRIHGRAAASAQGAVAPGPDGGGPFPPWPLG
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018337
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018337.2, NP_060807.2
• RefSeq Size: 2059 bp
• RefSeq ORF: 984 bp
• Locus ID: 55311
• UniProt ID: Q8N0Y2
• Cytogenetics: 19q13.43
• Domains: LER, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a zinc finger protein which activates transcription of a scavenger receptor gene involved in the degradation of acetylated low density lipoprotein (Ac-LDL) (PMID: 11978792). This gene is located in a cluster of zinc finger genes on chromosome 19 at q13.4. A pseudogene of this gene is located on chromosome 15. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]