ABCG2 (NM_004827) Human Tagged ORF Clone
ABCG2 (GFP-tagged) - Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)
|Product Name||ABCG2 (NM_004827) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG205640 representing NM_004827
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG205640 representing NM_004827
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1965 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||4445 bp|
|RefSeq ORF||1968 bp|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transmembrane|
|Protein Pathways||ABC transporters|
|Gene Summary||The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]|
|The use of this cDNA Clones has been cited in the following citations:|
Correlation between Membrane Protein Expression Levels and Transcellular Transport Activity for Breast Cancer Resistance Protein
,Liu, H;Huang, L;Li, Y;Fu, T;Sun, X;Zhang, YY;Gao, R;Chen, Q;Zhang, W;Sahi, J;Summerfield, S;Dong, K;,
Drug Metab. Dispos. 2017
,PubMed ID 28209803
|cDNA Clone Resources|
|SC117127||ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)||
|SC320948||ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)||
|RC205640||ABCG2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)||
|RC205640L1||Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged||
|RC205640L2||Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged||
|RC205640L3||Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged||
|RC205640L4||Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged||