USP44 (NM_032147) Human Tagged ORF Clone

CAT#: RG205560

USP44 (tGFP-tagged) - Human ubiquitin specific peptidase 44 (USP44), transcript variant 1

Product Images

Circular map for RG205560

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP44
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205560 representing NM_032147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGCAATGGATACGTGCAAACATGTTGGGCAGCTGCAGCTTGCTCAAGACCATTCCAGCCTCAACC
  CTCAGAAATGGCACTGTGTGGACTGCAACACGACCGAGTCCATTTGGGCTTGCCTTAGCTGCTCCCATGT
  TGCCTGTGGAAGATATATTGAAGAGCATGCACTCAAGCACTTTCAAGAAAGCAGTCATCCTGTTGCATTG
  GAGGTGAATGAGATGTACGTTTTTTGTTACCTTTGTGATGATTATGTTCTGAATGATAACGCAACTGGAG
  ACCTGAAGTTACTACGACGTACATTAAGTGCCATCAAAAGTCAAAATTATCACTGCACAACTCGTAGTGG
  GAGGTTTTTACGGTCCATGGGTACAGGTGATGATTCTTATTTCTTACATGACGGTGCCCAATCTCTGCTT
  CAAAGTGAAGATCAACTGTATACTGCTCTTTGGCACAGGAGAAGGATACTAATGGGTAAAATCTTTCGAA
  CATGGTTTGAACAATCACCCATTGGAAGAAAAAAGCAAGAAGAACCATTTCAGGAAAAAATAGTAGTAAA
  AAGAGAAGTAAAGAAAAGACGGCAGGAATTGGAGTATCAAGTTAAAGCAGAATTGGAAAGTATGCCTCCA
  AGAAAGAGTTTACGTTTACAAGGGCTCGCTCAGTCGACCATAATAGAAATAGTTTCTGTTCAGGTGCCAG
  CACAAACGCCAGCATCACCAGCAAAAGATAAAGTACTCTCTACCTCAGAAAATGAAATATCTCAAAAAGT
  CAGTGACTCCTCAGTTAAACGAAGGCCAATAGTAACTCCTGGTGTAACAGGATTGAGAAATTTGGGAAAT
  ACTTGCTATATGAATTCTGTTCTTCAGGTATTGAGTCATTTACTTATTTTTCGACAATGTTTTTTAAAGC
  TTGATCTGAACCAATGGCTGGCTATGACTGCTAGCGAGAAGACAAGATCTTGTAAGCATCCACCAGTCAC
  AGATACAGTAGTATATCAAATGAATGAATGTCAGGAAAAAGATACAGGTTTTGTTTGCTCCAGACAATCA
  AGTCTGTCATCAGGACTAAGTGGTGGAGCATCAAAAGGTAGAAAGATGGAACTTATTCAGCCAAAGGAGC
  CAACTTCACAGTACATTTCTCTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGTGGTCTGGAAAGTG
  GGCGTTGGTCTCACCATTTGCTATGCTACACTCAGTGTGGAGACTCATTCCTGCCTTTCGTGGTTACGCC
  CAACAAGACGCTCAGGAATTTCTTTGTGAACTTTTAGATAAAATACAACGTGAATTAGAGACAACTGGTA
  CCAGTTTACCAGCTCTTATCCCCACTTCTCAAAGGAAACTCATCAAACAAGTTCTGAATGTTGTAAATAA
  CATTTTTCATGGACAACTTCTTAGTCAGGTTACATGTCTTGCATGTGACAACAAATCAAATACCATAGAA
  CCTTTCTGGGACTTGTCATTGGAGTTTCCAGAAAGGTATCAATGCAGTGGAAAAGATATTGCTTCCCAGC
  CATGTCTGGTTACTGAAATGTTGGCCAAATTTACAGAAACTGAAGCTTTAGAAGGAAAAATCTACGTATG
  TGACCAGTGTAACTCAAAGCGTAGAAGGTTTTCCTCCAAACCAGTTGTACTCACAGAAGCCCAGAAACAA
  CTTATGATATGCCACCTACCTCAGGTTCTCAGACTGCACCTCAAACGATTCAGGTGGTCAGGACGTAATA
  ACCGAGAGAAGATTGGTGTTCATGTTGGCTTTGAGGAAATCTTAAACATGGAGCCCTATTGCTGCAGGGA
  GACCCTGAAATCCCTCAGACCAGAATGCTTTATCTATGACTTGTCCGCGGTGGTGATGCACCATGGGAAA
  GGATTTGGCTCAGGGCACTACACTGCCTACTGCTATAATTCTGAAGGAGGGTTCTGGGTACACTGCAATG
  ATTCCAAACTAAGCATGTGCACTATGGACGAAGTATGCAAGGCTCAAGCTTATATCTTGTTTTATACCCA
  ACGAGTTACTGAGAATGGACATTCTAAACTTTTGCCTCCAGAGCTCCTGTTGGGGAGCCAACATCCCAAT
  GAAGACGCTGATACCTCGTCTAATGAAATCCTTAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205560 representing NM_032147
  Red=Cloning site Green=Tags(s)
  
  MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKHFQESSHPVAL
  EVNEMYVFCYLCDDYVLNDNATGDLKLLRRTLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLL
  QSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPP
  RKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTPGVTGLRNLGN
  TCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQS
  SLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYA
  QQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIE
  PFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSKPVVLTEAQKQ
  LMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNMEPYCCRETLKSLRPECFIYDLSAVVMHHGK
  GFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPN
  EDADTSSNEILS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032147
• ORF Size: 2136 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032147.2, NP_115523.2
• RefSeq Size: 3476 bp
• RefSeq ORF: 2139 bp
• Locus ID: 84101
• UniProt ID: Q9H0E7
• Cytogenetics: 12q22
• Domains: UCH, zf-UBP
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. [provided by RefSeq, Dec 2016]