GLE1 (NM_001003722) Human Tagged ORF Clone

CAT#: RG205354

GLE1 (tGFP-tagged) - Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 1

Product Images

Circular map for RG205354

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GLE1
• Synonyms: CAAHC; CAAHD; GLE1L; hGLE1; LCCS; LCCS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205354 representing NM_001003722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCTGAGGGTCGCTGCTGGGAGACCTTGAAGGCCCTACGCAGTTCCGACAAAGGTCGCCTTTGCT
  ACTACCGCGACTGGCTGCTGCGGCGCGAGGATGTTTTAGAAGAATGTATGTCTCTTCCCAAGCTATCTTC
  TTATTCTGGATGGGTGGTAGAGCACGTCCTACCCCATATGCAGGAGAACCAACCTCTGTCTGAGACTTCG
  CCATCCTCTACGTCAGCTTCAGCCCTAGATCAACCCTCATTTGTTCCCAAATCTCCTGACGCAAGCTCTG
  CCTTTTCCCCAGCCTCCCCTGCAACACCAAATGGAACCAAGGGCAAAGATGAGTCCCAGCACACAGAATC
  TATGGTACTTCAGTCCTCACGGGGGATCAAAGTGGAAGACTGCGTCCGAATGTACGAACTGGTACACAGA
  ATGAAAGGAACAGAGGGCCTGAGGCTATGGCAGGAGGAGCAGGAGAGGAAGGTGCAAGCCCTCTCGGAGA
  TGGCATCTGAACAACTGAAGCGGTTTGATGAATGGAAGGAACTGAAGCAGCATAAAGAATTCCAGGACTT
  GCGGGAAGTAATGGAGAAGAGCTCCAGAGAAGCCTTGGGACACCAAGAGAAGCTAAAAGCTGAGCACCGT
  CACAGAGCAAAGATTCTCAACCTGAAGCTGCGGGAAGCAGAGCAGCAGCGCGTGAAGCAAGCAGAACAGG
  AGCGGCTTCGGAAGGAAGAAGGCCAGATCCGCCTGCGGGCCCTCTATGCTCTGCAGGAGGAGATGCTGCA
  GCTCAGCCAGCAGCTGGATGCCTCTGAGCAGCACAAAGCCCTGCTTAAGGTCGACCTGGCTGCCTTCCAG
  ACCCGAGGCAACCAGCTGTGCAGCCTCATCTCAGGGATCATCCGGGCCTCTTCAGAGAGCAGCTATCCCA
  CAGCAGAGAGTCAAGCTGAGGCTGAGCGAGCTCTGCGGGAAATGCGGGACCTCCTGATGAACTTGGGGCA
  GGAGATCACCAGAGCCTGCGAAGACAAGAGGAGGCAGGATGAAGAAGAGGCCCAGGTAAAGCTGCAAGAG
  GCACAGATGCAGCAGGGACCAGAGGCCCACAAAGAGCCCCCAGCTCCCAGCCAGGGCCCAGGAGGGAAAC
  AGAATGAAGACCTCCAGGTGAAGGTACAAGACATTACAATGCAGTGGTACCAGCAGCTGCAGGATGCTTC
  CATGCAGTGTGTGTTGACCTTTGAGGGCCTGACCAACAGCAAGGACAGTCAGGCCAAAAAGATAAAGATG
  GACCTCCAGAAGGCTGCTACCATCCCAGTGAGCCAAATCTCTACCATTGCAGGCTCAAAACTGAAGGAGA
  TCTTTGACAAGATCCACAGCCTGCTCTCTGGAAAACCTGTTCAATCTGGTGGGCGCTCTGTGTCTGTCAC
  ACTTAACCCACAGGGGCTGGACTTTGTTCAATACAAACTGGCAGAGAAATTTGTGAAACAAGGCGAGGAG
  GAAGTGGCCTCTCACCATGAAGCAGCATTCCCCATTGCAGTTGTGGCATCCGGGATCTGGGAGCTCCACC
  CCAGAGTGGGGGACCTCATTCTTGCTCATCTACATAAGAAGTGTCCTTACTCTGTTCCTTTCTATCCCAC
  TTTCAAGGAGGGAATGGCTTTGGAAGACTATCAGAGGATGCTTGGTTACCAAGTAAAGGATTCCAAAGTG
  GAGCAGCAAGACAACTTTCTAAAACGCATGTCAGGGATGATCCGTCTCTACGCTGCTATCATCCAGCTCC
  GGTGGCCATATGGAAACCAACAGGAGATTCACCCTCATGGCTTAAATCATGGATGGCGCTGGTTGGCACA
  GATCTTAAACATGGAGCCCTTGTCAGATGTGACAGCCACCCTCCTCTTTGACTTCCTGGAGGTGTGTGGG
  AATGCCCTCATGAAGCAATACCAGGTTCAGTTCTGGAAGATGCTAATTCTCATCAAAGAGGACTACTTTC
  CCAGAATTGAAGCTATCACAAGCTCAGGACAGATGGGCTCCTTCATACGCCTCAAGCAGTTCTTGGAGAA
  ATGTTTGCAACACAAGGACATTCCTGTCCCCAAGGGCTTTCTGACTTCCTCCTTCTGGCGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205354 representing NM_001003722
  Red=Cloning site Green=Tags(s)
  
  MPSEGRCWETLKALRSSDKGRLCYYRDWLLRREDVLEECMSLPKLSSYSGWVVEHVLPHMQENQPLSETS
  PSSTSASALDQPSFVPKSPDASSAFSPASPATPNGTKGKDESQHTESMVLQSSRGIKVEDCVRMYELVHR
  MKGTEGLRLWQEEQERKVQALSEMASEQLKRFDEWKELKQHKEFQDLREVMEKSSREALGHQEKLKAEHR
  HRAKILNLKLREAEQQRVKQAEQERLRKEEGQIRLRALYALQEEMLQLSQQLDASEQHKALLKVDLAAFQ
  TRGNQLCSLISGIIRASSESSYPTAESQAEAERALREMRDLLMNLGQEITRACEDKRRQDEEEAQVKLQE
  AQMQQGPEAHKEPPAPSQGPGGKQNEDLQVKVQDITMQWYQQLQDASMQCVLTFEGLTNSKDSQAKKIKM
  DLQKAATIPVSQISTIAGSKLKEIFDKIHSLLSGKPVQSGGRSVSVTLNPQGLDFVQYKLAEKFVKQGEE
  EVASHHEAAFPIAVVASGIWELHPRVGDLILAHLHKKCPYSVPFYPTFKEGMALEDYQRMLGYQVKDSKV
  EQQDNFLKRMSGMIRLYAAIIQLRWPYGNQQEIHPHGLNHGWRWLAQILNMEPLSDVTATLLFDFLEVCG
  NALMKQYQVQFWKMLILIKEDYFPRIEAITSSGQMGSFIRLKQFLEKCLQHKDIPVPKGFLTSSFWRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003722
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003722.1, NP_001003722.1
• RefSeq Size: 3350 bp
• RefSeq ORF: 2097 bp
• Locus ID: 2733
• UniProt ID: Q53GS7
• Cytogenetics: 9q34.11
• Gene Summary: This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]