VILIP1 (VSNL1) (NM_003385) Human Tagged ORF Clone

CAT#: RG205337

VSNL1 (tGFP-tagged) - Human visinin-like 1 (VSNL1)

Product Images

Circular map for RG205337

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VILIP1
• Synonyms: HLP3; HPCAL3; HUVISL1; VILIP; VILIP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205337 representing NM_003385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGCAGAATAGCAAACTGGCCCCTGAAGTGATGGAGGACCTGGTGAAGAGCACAGAGTTTAATG
  AGCATGAACTCAAGCAGTGGTACAAAGGATTTCTCAAGGACTGTCCAAGTGGGAGGCTAAATCTCGAGGA
  ATTTCAGCAGCTCTATGTGAAGTTCTTTCCTTATGGAGACGCCTCCAAGTTTGCCCAGCATGCCTTCCGA
  ACCTTCGACAAGAATGGGGACGGCACCATTGACTTCCGAGAGTTCATCTGCGCTCTGTCCATCACCTCCA
  GGGGCAGCTTTGAGCAGAAGCTGAACTGGGCCTTCAATATGTATGACCTGGATGGTGATGGCAAGATCAC
  CCGAGTGGAGATGCTGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGATCATGATGAAAATG
  AATGAGGATGGCCTGACGCCTGAGCAGCGAGTAGACAAGATTTTCAGCAAGATGGATAAGAACAAAGATG
  ACCAGATTACACTGGATGAATTCAAAGAAGCTGCAAAGAGCGACCCTTCCATTGTATTACTTCTGCAGTG
  CGACATCCAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205337 representing NM_003385
  Red=Cloning site Green=Tags(s)
  
  MGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFR
  TFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKM
  NEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003385
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003385.5
• RefSeq Size: 2014 bp
• RefSeq ORF: 576 bp
• Locus ID: 7447
• UniProt ID: P62760
• Cytogenetics: 2p24.2
• Domains: EFh
• Protein Families: Druggable Genome
• Gene Summary: This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]