Epac2 (RAPGEF4) (NM_007023) Human Tagged ORF Clone

CAT#: RG205335

RAPGEF4 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1

Product Images

Circular map for RG205335

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Epac2
• Synonyms: CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205335 representing NM_007023
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGCTGCGCACGCTGCCCATTCTTCCTCCTCTGCCGAGTGGATCGCCTGCCTGGATAAAAGACCAC
  TGGAGCGATCCAGCGAAGATGTGGATATAATCTTCACTCGACTGAAAGAAGTTAAAGCTTTTGAGAAATT
  TCACCCAAATCTCCTTCATCAGATTTGCTTATGTGGTTATTATGAGAATCTGGAAAAGGGAATAACATTA
  TTTCGCCAGGGTGATATTGGAACAAACTGGTATGCTGTCCTGGCAGGGTCTTTGGATGTTAAAGTATCTG
  AGACCAGCAGTCACCAGGATGCTGTGACCATCTGTACCCTGGGAATTGGGACGGCCTTTGGAGAGTCCAT
  TCTGGACAACACACCCCGCCATGCAACCATCGTTACCAGGGAGAGCAGTGAACTGCTCCGCATCGAGCAG
  AAGGACTTCAAGGCACTATGGGAGAAATATCGACAGTATATGGCAGGACTTCTGGCTCCTCCTTATGGTG
  TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAACACACCTCTCATTGAACCTCACGT
  TCCTCTTCGTCCTGCTAACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTA
  CGAAATGCCATTCTCTCTCGAGCACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAGAC
  AATGCTGTGTGGGAACTGAACTGGTGGACTGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCA
  AGCTGTTGGCATGTGGCAAGTCCTGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTC
  CAAGACAAATATTTATTCTATCGATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGG
  AGAAGAAGGAGTGTGATGAGGAGCTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCA
  CATGAGGATGATCCTTCGCAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAG
  CTTCTTCATATTAAAGCCTTATCCCATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTT
  TTGAGTCTCACGCCAAAGGAGGGACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTAT
  TCTAAAAGGATCAGTGAATGTAGTCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGAC
  TTCGGCAAGTTAGCACTAGTGAATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCC
  ATTTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACT
  TAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGA
  AACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAG
  AAACAATACGCCTTGAGGCAACTTTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCA
  CTGTGTTTTTATGCCAAATACCCAGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAA
  GGTACAGAACAGGAGAAAATGGATTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGT
  GGGCTGCCATGTATGGAGACCTCCTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGT
  ATCTGTATCAGATGATGCCCGGATGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTC
  AAGCAAATCTCAGAAGATGCAAAGGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGG
  GCGATGAGAGAGCCCAGAAGCGCCAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCAT
  GGACCCCACCTACACAACCATTCGGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCC
  GACAAGCTGGGCTCCGGGGAGGGCCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCA
  AACCTAATGATGTTTCAGTATTTATGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCA
  ATTCGATTCACTGACTCCCTTACCAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTG
  ATGAGCTCCAAAGATTTAGCATACCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGC
  TGGAGCTAATCTATCACACATTTGGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCT
  GAGGAGATTTAATGAAATTCAGTTTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGT
  GTTCAGCTATTAAAAAAATTTATTAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTT
  TTGCCATCGTCATGGGACTAAGTAACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAG
  CAAGTTCAAGAAGTTCTATGCGGAGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGG
  CTGACAGTAGCTAAGCTGGAACCTCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTA
  CTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATAC
  GGCCAGAACAGTGAGATACTACAGGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCAT
  CAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCAC
  ACAGATTAGAGCCTCGTCGACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205335 representing NM_007023
  Red=Cloning site Green=Tags(s)
  
  MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITL
  FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQ
  KDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKIL
  RNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHF
  QDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEE
  LLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDD
  FGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQG
  NSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQ
  GTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIV
  KQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDPTYTTIRVPVATSVKEVISAVA
  DKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFMTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFEL
  MSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKR
  VQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYR
  LTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNH
  QDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007023
• ORF Size: 3033 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007023.1, NP_008954.1
• RefSeq Size: 4278 bp
• RefSeq ORF: 3036 bp
• Locus ID: 11069
• UniProt ID: Q8WZA2
• Cytogenetics: 2q31.1
• Domains: DEP, cNMP, RasGEFN, RasGEF
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function]