STK36 (NM_015690) Human Tagged ORF Clone

CAT#: RG205254

STK36 (tGFP-tagged) - Human serine/threonine kinase 36 (STK36)

Product Images

Circular map for RG205254

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STK36
• Synonyms: FU
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205254 representing NM_015690
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGTACCACGTGTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGGGTGTACAAGGGTCGAAGAA
  AATACAGTGCTCAGGTCGTGGCCCTGAAGTTCATCCCAAAATTGGGGCGCTCAGAGAAGGAGCTGAGGAA
  TTTGCAACGAGAGATTGAAATAATGCGGGGTCTGCGGCATCCCAACATTGTGCATATGCTTGACAGCTTT
  GAAACTGATAAAGAGGTGGTGGTGGTGACAGACTATGCTGAGGGAGAGCTCTTTCAGATCCTAGAAGATG
  ACGGAAAACTTCCTGAAGACCAGGTTCAGGCCATTGCTGCCCAGTTGGTGTCAGCCCTGTACTATCTGCA
  TTCCCACCGCATCCTACACCGAGATATGAAGCCTCAGAACATCCTCCTCGCCAAGGGTGGTGGCATCAAG
  CTCTGTGACTTTGGATTTGCCCGGGCTATGAGCACCAATACAATGGTGCTGACATCCATCAAAGGCACAC
  CACTCTATATGTCTCCAGAGCTGGTGGAGGAGCGACCATACGACCACACAGCGGACCTCTGGTCTGTTGG
  CTGCATACTATATGAACTGGCAGTAGGCACCCCTCCCTTCTATGCTACAAGCATCTTTCAGCTGGTCAGC
  CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCAACCATCAGTCCCTGCTTTAAGGACTTCCTGCAGGGAC
  TGCTCACCAAAGACCCACGGCAGCGACTGTCCTGGCCAGACCTCTTATATCACCCCTTTATTGCTGGTCA
  TGTCACCATAATAACTGAGCCAGCAGGCCCAGATTTGGGGACCCCATTCACCAGCCGCCTACCCCCAGAA
  CTTCAGGTCCTAAAGGACGAACAGGCCCATCGGTTGGCCCCCAAGGGTAATCAGTCTCGCATCTTGACTC
  AGGCCTATAAACGCATGGCTGAGGAGGCCATGCAGAAGAAACATCAGAACACAGGACCTGCCCTTGAGCA
  AGAGGACAAGACCAGCAAGGTGGCTCCTGGCACAGCCCCTCTGCCCAGACTCGGGGCCACTCCTCAGGAA
  TCAAGCCTCCTGGCCGGGATCTTAGCCTCAGAATTGAAGAGCAGCTGGGCTAAATCAGGGACTGGAGAGG
  TGCCCTCTGCACCTCGGGAAAACCGGACCACCCCAGATTGTGAACGAGCATTCCCAGAGGAGAGGCCAGA
  GGTGCTGGGCCAGCGGAGCACTGATGTAGTGGACCTGGAAAATGAGGAGCCAGACAGTGACAATGAGTGG
  CAGCACCTGCTAGAGACCACTGAGCCTGTGCCTATTCAACTGAAGGCTCCTCTCACCTTGCTGTGTAATC
  CTGACTTCTGCCAGCGCATCCAGAGTCAGCTGCATGAAGCTGGAGGGCAGATCCTGAATGGCATCTTGGA
  GGGTGCTTCCCACATCCTGCCTGCATTCCGGGTCCTGAGCAGTCTTCTCTCCAGCTGCAGTGATTCTGTT
  GCCTTGTATTCCTTCTGCCGGGAGGCAGGGCTTCCTGGGCTGCTGCTGAGTCTACTCAGGCACAGTCAGG
  AGAGCAACAGCCTCCAGCAGCAATCTTGGTATGGGACCTTCTTACAGGACCTGATGGCTGTGATTCAGGC
  CTACTTTGCCTGTACCTTCAATCTGGAGAGGAGCCAGACAAGTGACAGCCTGCAGGTGTTTCAGGAGGCT
  GCCAACCTTTTTCTGGACCTGTTGGGGAAACTGCTGGCCCAACCAGATGACTCTGAGCAGACTTTGCGGA
  GGGACAGCCTTATGTGCTTTACTGTCCTGTGCGAAGCCATGGATGGGAACAGCCGGGCCATCTCCAAAGC
  CTTTTACTCCAGCTTGCTGACGACACAGCAGGTTGTCTTGGATGGGCTCCTTCATGGCTTGACAGTTCCA
  CAGCTCCCTGTCCACACTCCCCAAGGAGCCCCGCAAGTGAGCCAGCCACTGCGAGAGCAGAGTGAGGATA
  TACCTGGAGCCATTTCCTCTGCCCTGGCAGCCATATGCACTGCTCCTGTGGGACTGCCCGACTGCTGGGA
  TGCCAAGGAGCAGGTCTGTTGGCATTTGGCAAATCAGCTAACTGAAGACAGCAGCCAGCTCAGGCCATCC
  CTCATCTCTGGCCTGCAGCATCCCATCCTGTGCCTGCACCTTCTCAAGGTTCTATACTCCTGCTGCCTTG
  TCAGTGAGGGCCTGTGCCGTCTTCTGGGGCAGGAGCCCCTGGCCTTGGAATCCCTGTTTATGTTGATTCA
  GGGCAAGGTAAAAGTAGTAGATTGGGAAGAGTCTACTGAAGTGACACTCTACTTCCTCACCCTTCTTGTC
  TTTCGGCTCCAAAACCTGCCTTGTGGAATGGAGAAGCTAGGCAGTGACGTTGCTACTCTCTTTACCCATT
  CGCATGTCGTCTCTCTTGTGAGTGCAGCAGCCTGTCTATTGGGACAGCTTGGTCAGCAAGGGGTGACCTT
  TGACCTCCAGCCCATGGAATGGATGGCTGCAGCCACACATGCCTTGTCTGCCCCTGCAGAGGTTCGGTTG
  ACTCCACCAGGTAGTTGTGGATTCTATGATGGCCTCCTTATCCTTCTGTTGCAGCTCCTCACTGAGCAGG
  GGAAGGCTAGCCTAATCAGGGATATGTCCAGTTCAGAAATGTGGACCGTTTTGTGGCACCGCTTCTCCAT
  GGTCCTGAGGCTCCCCGAGGAGGCATCTGCACAGGAAGGGGAGCTTTCGCTATCCAGTCCACCAAGCCCT
  GAGCCAGACTGGACACTGATTTCTCCCCAGGGCATGGCAGCCCTGCTGAGCCTGGCCATGGCCACCTTTA
  CCCAGGAGCCCCAGTTATGCCTGAGCTGCCTGTCCCAGCATGGAAGTATCCTCATGTCCATCCTGAAGCA
  TCTGCTTTGCCCCAGCTTCCTGAATCAACTGCGCCAGGCGCCTCATGGGTCTGAGTTTCTCCCTGTCGTG
  GTGCTCTCTGTCTGCCAGCTCCTTTGCTTCCCCTTTGCGCTGGACATGGATGCTGACCTCCTTATAGGTG
  TCTTGGCCGACCTCAGGGACTCAGAAGTTGCAGCCCATCTGCTGCAGGTCTGCTGCTACCATCTTCCGTT
  GATGCAAGTGGAGCTGCCCATCAGCCTTCTCACACGCCTGGCCCTCATGGATCCCACCTCTCTCAACCAG
  TTTGTGAACACAGTGTCTGCCTCCCCTAGAACCATCGTCTCGTTTCTCTCAGTTGCCCTCCTGAGTGACC
  AGCCACTGTTGACCTCCGACCTTCTCTCTCTGCTGGCCCATACTGCCAGGGTCCTGTCTCCCAGCCACTT
  GTCCTTTATCCAAGAGCTTCTGGCTGGCTCTGATGAATCCTATCGGCCCCTGCGCAGCCTCCTGGGCCAC
  CCAGAGAATTCTGTGCGGGCACACACTTATAGGCTCCTGGGACACTTGCTCCAACACAGCATGGCCCTGC
  GTGGGGCACTGCAGAGCCAGTCTGGACTGCTCAGCCTTCTGCTGCTTGGGCTTGGAGACAAGGATCCTGT
  TGTGCGGTGCAGTGCCAGCTTTGCTGTGGGCAATGCAGCCTACCAGGCTGGTCCTCTGGGACCTGCCCTG
  GCAGCTGCAGTGCCCAGTATGACCCAGCTGCTTGGAGATCCTCAGGCTGGTATCCGGCGCAATGTTGCAT
  CAGCTCTGGGCAACTTGGGACCTGAAGGTTTGGGAGAGGAGCTGTTACAGTGCGAAGTACCCCAGCGGCT
  CCTAGAAATGGCATGTGGAGACCCCCAGCCAAATGTGAAGGAGGCTGCCCTCATTGCCCTCCGGAGCCTG
  CAACAGGAGCCTGGCATCCATCAGGTACTGGTGTCCCTGGGTGCCAGTGAGAAACTATCCTTGCTCTCTC
  TGGGGAATCAGTCACTGCCACACAGCAGTCCTAGGCCTGCCTCTGCCAAACACTGCAGGAAACTCATTCA
  CCTCCTGAGGCCAGCCCATAGCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205254 representing NM_015690
  Red=Cloning site Green=Tags(s)
  
  MEKYHVLEMIGEGSFGRVYKGRRKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLRHPNIVHMLDSF
  ETDKEVVVVTDYAEGELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIK
  LCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYATSIFQLVS
  LILKDPVRWPSTISPCFKDFLQGLLTKDPRQRLSWPDLLYHPFIAGHVTIITEPAGPDLGTPFTSRLPPE
  LQVLKDEQAHRLAPKGNQSRILTQAYKRMAEEAMQKKHQNTGPALEQEDKTSKVAPGTAPLPRLGATPQE
  SSLLAGILASELKSSWAKSGTGEVPSAPRENRTTPDCERAFPEERPEVLGQRSTDVVDLENEEPDSDNEW
  QHLLETTEPVPIQLKAPLTLLCNPDFCQRIQSQLHEAGGQILNGILEGASHILPAFRVLSSLLSSCSDSV
  ALYSFCREAGLPGLLLSLLRHSQESNSLQQQSWYGTFLQDLMAVIQAYFACTFNLERSQTSDSLQVFQEA
  ANLFLDLLGKLLAQPDDSEQTLRRDSLMCFTVLCEAMDGNSRAISKAFYSSLLTTQQVVLDGLLHGLTVP
  QLPVHTPQGAPQVSQPLREQSEDIPGAISSALAAICTAPVGLPDCWDAKEQVCWHLANQLTEDSSQLRPS
  LISGLQHPILCLHLLKVLYSCCLVSEGLCRLLGQEPLALESLFMLIQGKVKVVDWEESTEVTLYFLTLLV
  FRLQNLPCGMEKLGSDVATLFTHSHVVSLVSAAACLLGQLGQQGVTFDLQPMEWMAAATHALSAPAEVRL
  TPPGSCGFYDGLLILLLQLLTEQGKASLIRDMSSSEMWTVLWHRFSMVLRLPEEASAQEGELSLSSPPSP
  EPDWTLISPQGMAALLSLAMATFTQEPQLCLSCLSQHGSILMSILKHLLCPSFLNQLRQAPHGSEFLPVV
  VLSVCQLLCFPFALDMDADLLIGVLADLRDSEVAAHLLQVCCYHLPLMQVELPISLLTRLALMDPTSLNQ
  FVNTVSASPRTIVSFLSVALLSDQPLLTSDLLSLLAHTARVLSPSHLSFIQELLAGSDESYRPLRSLLGH
  PENSVRAHTYRLLGHLLQHSMALRGALQSQSGLLSLLLLGLGDKDPVVRCSASFAVGNAAYQAGPLGPAL
  AAAVPSMTQLLGDPQAGIRRNVASALGNLGPEGLGEELLQCEVPQRLLEMACGDPQPNVKEAALIALRSL
  QQEPGIHQVLVSLGASEKLSLLSLGNQSLPHSSPRPASAKHCRKLIHLLRPAHSM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015690
• ORF Size: 3945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015690.2, NP_056505.1
• RefSeq Size: 4887 bp
• RefSeq ORF: 3948 bp
• Locus ID: 27148
• UniProt ID: Q9NRP7
• Cytogenetics: 2q35
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a member of the serine/threonine kinase family of enzymes. This family member is similar to a Drosophila protein that plays a key role in the Hedgehog signaling pathway. This human protein is a positive regulator of the GLI zinc-finger transcription factors. Knockout studies of the homologous mouse gene suggest that defects in this human gene may lead to congenital hydrocephalus, possibly due to a functional defect in motile cilia. Because Hedgehog signaling is frequently activated in certain kinds of gastrointestinal cancers, it has been suggested that this gene is a target for the treatment of these cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]