LGALS3BP (NM_005567) Human Tagged ORF Clone

CAT#: RG204918

LGALS3BP (tGFP-tagged) - Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP)

Product Images

Circular map for RG204918

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LGALS3BP
• Synonyms: 90K; BTBD17B; CyCAP; gp90; M2BP; MAC-2-BP; TANGO10B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204918 representing NM_005567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCTCCGAGGCTCTTCTGGGTGTGGCTGCTGGTTGCAGGAACCCAAGGCGTGAACGATGGTGACA
  TGCGGCTGGCCGATGGGGGCGCCACCAACCAGGGCCGCGTGGAGATCTTCTACAGAGGCCAGTGGGGCAC
  TGTGTGTGACAACCTGTGGGACCTGACTGATGCCAGCGTCGTCTGCCGGGCCCTGGGCTTCGAGAACGCC
  ACCCAGGCTCTGGGCAGAGCTGCCTTCGGGCAAGGATCAGGCCCCATCATGCTGGACGAGGTCCAGTGCA
  CGGGAACCGAGGCCTCACTGGCCGACTGCAAGTCCCTGGGCTGGCTGAAGAGCAACTGCAGGCACGAGAG
  AGACGCTGGTGTGGTCTGCACCAATGAAACCAGGAGCACCCACACCCTGGACCTCTCCAGGGAGCTCTCG
  GAGGCCCTTGGCCAGATCTTTGACAGCCAGCGGGGCTGCGACCTGTCCATCAGCGTGAATGTGCAGGGCG
  AGGACGCCCTGGGCTTCTGTGGCCACACGGTCATCCTGACTGCCAACCTGGAGGCCCAGGCCCTGTGGAA
  GGAGCCGGGCAGCAATGTCACCATGAGTGTGGATGCTGAGTGTGTGCCCATGGTCAGGGACCTTCTCAGG
  TACTTCTACTCCCGAAGGATTGACATCACCCTGTCGTCAGTCAAGTGCTTCCACAAGCTGGCCTCTGCCT
  ATGGGGCCAGGCAGCTGCAGGGCTACTGCGCAAGCCTCTTTGCCATCCTCCTCCCCCAGGACCCCTCGTT
  CCAGATGCCCCTGGACCTGTATGCCTATGCAGTGGCCACAGGGGACGCCCTGCTGGAGAAGCTCTGCCTA
  CAGTTCCTGGCCTGGAACTTCGAGGCCTTGACGCAGGCCGAGGCCTGGCCCAGTGTCCCCACAGACCTGC
  TCCAACTGCTGCTGCCCAGGAGCGACCTGGCGGTGCCCAGCGAGCTGGCCCTACTGAAGGCCGTGGACAC
  CTGGAGCTGGGGGGAGCGTGCCTCCCATGAGGAGGTGGAGGGCTTGGTGGAGAAGATCCGCTTCCCCATG
  ATGCTCCCTGAGGAGCTCTTTGAGCTGCAGTTCAACCTGTCCCTGTACTGGAGCCACGAGGCCCTGTTCC
  AGAAGAAGACTCTGCAGGCCCTGGAATTCCACACTGTGCCCTTCCAGTTGCTGGCCCGGTACAAAGGCCT
  GAACCTCACCGAGGATACCTACAAGCCCCGGATTTACACCTCGCCCACCTGGAGTGCCTTTGTGACAGAC
  AGTTCCTGGAGTGCACGGAAGTCACAACTGGTCTATCAGTCCAGACGGGGGCCTTTGGTCAAATATTCTT
  CTGATTACTTCCAAGCCCCCTCTGACTACAGATACTACCCCTACCAGTCCTTCCAGACTCCACAACACCC
  CAGCTTCCTCTTCCAGGACAAGAGGGTGTCCTGGTCCCTGGTCTACCTCCCCACCATCCAGAGCTGCTGG
  AACTACGGCTTCTCCTGCTCCTCGGACGAGCTCCCTGTCCTGGGCCTCACCAAGTCTGGCGGCTCAGATC
  GCACCATTGCCTACGAAAACAAAGCCCTGATGCTCTGCGAAGGGCTCTTCGTGGCAGACGTCACCGATTT
  CGAGGGCTGGAAGGCTGCGATTCCCAGTGCCCTGGACACCAACAGCTCGAAGAGCACCTCCTCCTTCCCC
  TGCCCGGCAGGGCACTTCAACGGCTTCCGCACGGTCATCCGCCCCTTCTACCTGACCAACTCCTCAGGTG
  TGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204918 representing NM_005567
  Red=Cloning site Green=Tags(s)
  
  MTPPRLFWVWLLVAGTQGVNDGDMRLADGGATNQGRVEIFYRGQWGTVCDNLWDLTDASVVCRALGFENA
  TQALGRAAFGQGSGPIMLDEVQCTGTEASLADCKSLGWLKSNCRHERDAGVVCTNETRSTHTLDLSRELS
  EALGQIFDSQRGCDLSISVNVQGEDALGFCGHTVILTANLEAQALWKEPGSNVTMSVDAECVPMVRDLLR
  YFYSRRIDITLSSVKCFHKLASAYGARQLQGYCASLFAILLPQDPSFQMPLDLYAYAVATGDALLEKLCL
  QFLAWNFEALTQAEAWPSVPTDLLQLLLPRSDLAVPSELALLKAVDTWSWGERASHEEVEGLVEKIRFPM
  MLPEELFELQFNLSLYWSHEALFQKKTLQALEFHTVPFQLLARYKGLNLTEDTYKPRIYTSPTWSAFVTD
  SSWSARKSQLVYQSRRGPLVKYSSDYFQAPSDYRYYPYQSFQTPQHPSFLFQDKRVSWSLVYLPTIQSCW
  NYGFSCSSDELPVLGLTKSGGSDRTIAYENKALMLCEGLFVADVTDFEGWKAAIPSALDTNSSKSTSSFP
  CPAGHFNGFRTVIRPFYLTNSSGVD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005567
• ORF Size: 1755 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005567.2, NP_005558.1
• RefSeq Size: 2254 bp
• RefSeq ORF: 1758 bp
• Locus ID: 3959
• UniProt ID: Q08380
• Cytogenetics: 17q25.3
• Domains: SR
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]