GIMAP4 (NM_018326) Human Tagged ORF Clone

CAT#: RG204653

GIMAP4 (tGFP-tagged) - Human GTPase, IMAP family member 4 (GIMAP4)

Product Images

Circular map for RG204653

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GIMAP4
• Synonyms: IAN-1; IAN1; IMAP4; MSTP062
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204653 representing NM_018326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCAATACGGCAGTATGAGCTTCAACCCCAGCACACCAGGGGCCAGTTATGGGCCTGGAAGGC
  AAGAGCCCAGAAATTCCCAATTGAGAATTGTGTTAGTGGGTAAAACCGGAGCAGGAAAAAGTGCAACAGG
  AAACAGCATCCTTGGCCGGAAAGTGTTTCATTCTGGCACTGCAGCAAAATCCATTACCAAGAAGTGTGAG
  AAACGCAGCAGCTCATGGAAGGAAACAGAACTTGTCGTAGTTGACACACCAGGCATTTTCGACACAGAGG
  TGCCCAATGCTGAAACGTCCAAGGAGATTATTCGCTGCATTCTTCTGACCTCCCCAGGGCCTCATGCTCT
  GCTTCTGGTGGTTCCACTGGGCCGTTACACTGAGGAGGAGCACAAAGCCACAGAGAAGATCCTGAAAATG
  TTTGGAGAGAGGGCTAGAAGTTTCATGATTCTCATATTCACCCGGAAAGATGACTTAGGTGACACCAATT
  TGCATGACTACTTAAGGGAAGCTCCAGAAGACATTCAAGACTTGATGGACATTTTCGGTGACCGCTACTG
  TGCGTTAAACAACAAGGCAACAGGCGCTGAGCAGGAGGCCCAGAGGGCACAGTTGCTGGGCCTGATCCAG
  CGCGTGGTGAGGGAGAACAAGGAAGGCTGCTACACTAATAGGATGTACCAAAGGGCGGAGGAGGAGATCC
  AGAAGCAAACACAAGCAATGCAAGAACTCCACAGAGTGGAGCTGGAGAGAGAGAAAGCGCGGATAAGAGA
  GGAGTATGAAGAGAAAATCAGAAAGCTGGAAGATAAAGTGGAGCAGGAAAAGAGAAAGAAGCAAATGGAG
  AAGAAACTAGCAGAACAGGAGGCTCACTATGCTGTAAGGCAGCAAAGGGCAAGAACGGAAGTGGAGAGTA
  AGGATGGGATACTTGAATTAATCATGACAGCGTTACAGATTGCTTCCTTTATTTTGTTACGTCTGTTCGC
  GGAAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204653 representing NM_018326
  Red=Cloning site Green=Tags(s)
  
  MAAQYGSMSFNPSTPGASYGPGRQEPRNSQLRIVLVGKTGAGKSATGNSILGRKVFHSGTAAKSITKKCE
  KRSSSWKETELVVVDTPGIFDTEVPNAETSKEIIRCILLTSPGPHALLLVVPLGRYTEEEHKATEKILKM
  FGERARSFMILIFTRKDDLGDTNLHDYLREAPEDIQDLMDIFGDRYCALNNKATGAEQEAQRAQLLGLIQ
  RVVRENKEGCYTNRMYQRAEEEIQKQTQAMQELHRVELEREKARIREEYEEKIRKLEDKVEQEKRKKQME
  KKLAEQEAHYAVRQQRARTEVESKDGILELIMTALQIASFILLRLFAED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018326
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018326.2, NP_060796.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 990 bp
• Locus ID: 55303
• UniProt ID: Q9NUV9
• Cytogenetics: 7q36.1
• Domains: AIG1
• Gene Summary: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. The encoded protein of this gene may be negatively regulated by T-cell acute lymphocytic leukemia 1 (TAL1). In humans, the IAN subfamily genes are located in a cluster at 7q36.1. [provided by RefSeq, Jul 2008]