Nuclear Factor 1 (NFIA) (NM_005595) Human Tagged ORF Clone

CAT#: RG204650

NFIA (tGFP-tagged) - Human nuclear factor I/A (NFIA), transcript variant 2

Product Images

Circular map for RG204650

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nuclear Factor 1
• Synonyms: BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204650 representing NM_005595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCTCCGCTCTGTCTCACCCAGGATGAATTTCATCCTTTCATCGAAGCACTTCTGCCCCACGTCC
  GAGCCTTTGCCTACACATGGTTCAACCTGCAGGCCCGAAAACGAAAATACTTCAAAAAACATGAAAAGCG
  TATGTCAAAAGAAGAAGAGAGAGCCGTGAAGGATGAATTGCTAAGTGAAAAACCAGAGGTCAAGCAGAAG
  TGGGCATCTCGACTTCTGGCAAAGTTGCGGAAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTA
  CAGTTACAGGGAAAAAACCTCCATGTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGAAGAAT
  TGACTGCCTCCGCCAGGCAGATAAAGTCTGGAGGTTGGACCTTGTTATGGTGATTTTGTTTAAAGGTATT
  CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGTCCCCACAATGCTCTAATCCAGGGCTCTGTGTCC
  AACCCCATCACATAGGGGTTTCTGTTAAGGAACTCGATTTATATTTGGCATACTTTGTGCATGCAGCAGA
  TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGACGCTGACATTAAGGACCAGCCAGAAAATGGACAT
  TTGGGCTTCCAGGACAGTTTTGTCACATCAGGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGA
  CACCAATAGCTGCAGGAACTGGCCCAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTACAGCAT
  GAGTCCAGGAGCAATGAGGAGGTCTTTACCCAGCACATCCTCTACGAGCTCCACAAAGCGCCTCAAGTCT
  GTGGAGGATGAAATGGACAGTCCTGGTGAGGAGCCATTTTATACAGGCCAAGGGCGCTCCCCAGGAAGTG
  GCAGTCAGTCAAGTGGATGGCATGAAGTGGAGCCAGGAATGCCATCTCCAACCACACTGAAGAAGTCGGA
  GAAGTCTGGTTTCAGCAGCCCCTCCCCTTCACAGACCTCCTCCCTGGGAACGGCGTTCACACAGCATCAC
  CGACCTGTCATTACAGGACCCAGAGCAAGTCCGCATGCAACACCATCGACTCTTCATTTCCCGACATCAC
  CCATTATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACGCTGAA
  AGAATTTGTCCAACTTGTCTGCCCTGATGCTGGTCAGCAGGCTGGACAGGTGGGGTTCCTCAATCCCAAT
  GGGAGCAGCCAAGGCAAGGTGCACAACCCATTCCTTCCCACCCCAATGTTGCCACCGCCACCGCCACCAC
  CGATGGCCAGGCCTGTGCCTCTGCCGGTGCCAGACACAAAGCCTCCAACCACGTCAACAGAAGGAGGTGC
  AGCCTCCCCCACGTCACCAATCCTGGTACCTGGGATAAAAGTTGCAGCGTCCCACCATCCACCAGACAGA
  CCACCTGACCCCTTCTCAACTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204650 representing NM_005595
  Red=Cloning site Green=Tags(s)
  
  MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK
  WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI
  PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGH
  LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS
  VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHH
  RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
  GSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDR
  PPDPFSTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005595
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005595.5
• RefSeq Size: 2684 bp
• RefSeq ORF: 1497 bp
• Locus ID: 4774
• UniProt ID: Q12857
• Cytogenetics: 1p31.3
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]