ZDHHC11 (NM_024786) Human Tagged ORF Clone

CAT#: RG204383

ZDHHC11 (tGFP-tagged) - Human zinc finger, DHHC-type containing 11 (ZDHHC11)

Product Images

Circular map for RG204383

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZDHHC11
• Synonyms: ZNF399
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204383 representing NM_024786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCCGCTCCGGGAGCCAGTGTTCCGTCACCCCAGAAGCCATACTCAATAATGAAAAGCTGGTCT
  TGCCGCCCCGCATCTCCAGAGTGAACGGCTGGTCGTTACCCCTGCACTACTTCCAGGTGGTGACCTGGGC
  TGTCTTCGTGGGCCTTTCCTCGGCCACCTTCGGGATCTTCATTCCCTTCCTGCCTCACGCGTGGAAATAC
  ATTGCCTACGTGGTGACCGGGGGGATCTTCTCGTTCCACCTCGTCGTCCACCTGATCGCGTCCTGCATCG
  ACCCGGCCGACTCCAATGTCAGACTCATGAAGAACTATTCTCAGCCCATGCCCCTCTTCGACAGATCAAA
  ACATGCACACGTGATCCAGAATCAGTTCTGCCACCTGTGCAAGGTCACCGTGAACAAGAAAACCAAACAC
  TGCATTTCCTGCAATAAGTGTGTGTCCGGCTTCGACCACCACTGCAAATGGATCAACAACTGCGTGGGAA
  GCCGGAATTATTGGTTCTTCTTCAGCACTGTGGCCTCGGCCACAGCTGGCATGCTCTGCCTGATCGCCAT
  CCTGCTGTATGTCCTCGTCCAGTACCTCGTGAACCCCGGGGTGCTCCGCACGGACCCCAGGTATGAAGAT
  GTCAAGAATATGAACACGTGGCTGCTGTTCCTCCCCCTGTTCCCGGTGCAGGTGCAGACCCTGATAGTCG
  TGATCATCGGGATGCTCGTGCTCCTGCTGGACTTTCTTGGCTTGGTGCACCTGGGCCAGCTGCTCATCTT
  CCACATCTACCTGAAGGCCAAGAAGATGACCACCTTTGAGTATCTCATTAATAACCGCAAAGAAGAGAGT
  TCAAAACATCAAGCAGTGAGGAAAGATCCATACGTGCAAATGGACAAAGGAGTTCTCCAGCAAGGAGCTG
  GCGCCCTGGGCTCATCTGCACAGGGAGTCAAAGCCAAGAGCTCCCTGCTGATTCACAAGCACTTATGTCA
  CTTCTGCACTTCAGTAAACCAGGATGGGGATTCGACGGCACGGGAAGGGGATGAAGACCCGTGTCCATCT
  GCACTTGGAGCCAAGGCCAGGAACTCCCGGCTGATTTGCAGGCGCCTGTGTCAGTTCTCCACTCGTGTAC
  ACCCAGACGGGGGCTCGATGGCACAGGAAGCAGATGATGCCCCGAGTATATCTACACTTGGGCTGCAACA
  AGAAACAACAGAGCCCATGAAAACTGACAGTGCTGAAAGTGAAGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204383 representing NM_024786
  Red=Cloning site Green=Tags(s)
  
  MDTRSGSQCSVTPEAILNNEKLVLPPRISRVNGWSLPLHYFQVVTWAVFVGLSSATFGIFIPFLPHAWKY
  IAYVVTGGIFSFHLVVHLIASCIDPADSNVRLMKNYSQPMPLFDRSKHAHVIQNQFCHLCKVTVNKKTKH
  CISCNKCVSGFDHHCKWINNCVGSRNYWFFFSTVASATAGMLCLIAILLYVLVQYLVNPGVLRTDPRYED
  VKNMNTWLLFLPLFPVQVQTLIVVIIGMLVLLLDFLGLVHLGQLLIFHIYLKAKKMTTFEYLINNRKEES
  SKHQAVRKDPYVQMDKGVLQQGAGALGSSAQGVKAKSSLLIHKHLCHFCTSVNQDGDSTAREGDEDPCPS
  ALGAKARNSRLICRRLCQFSTRVHPDGGSMAQEADDAPSISTLGLQQETTEPMKTDSAESED
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024786
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024786.2
• RefSeq Size: 2623 bp
• RefSeq ORF: 1239 bp
• Locus ID: 79844
• UniProt ID: Q9H8X9
• Cytogenetics: 5p15.33
• Protein Families: Transmembrane
• Gene Summary: Endoplasmic reticulum-localized palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates and be involved in a variety of cellular processes (By similarity). Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation (By similarity). May play a role in cell proliferation (PubMed:28331227).[UniProtKB/Swiss-Prot Function]