HIPPI (IFT57) (NM_018010) Human Tagged ORF Clone

CAT#: RG204116

IFT57 (tGFP-tagged) - Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57)

Product Images

Circular map for RG204116

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIPPI
• Synonyms: ESRRBL1; HIPPI; MHS4R2; OFD18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204116 representing NM_018010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCTGCTCTGGCCGTCGTCACGACGTCGGGTTTGGAAGATGGGGTGCCTAGGTCCCGTGGCGAAG
  GGACCGGGGAAGTGGTCTTGGAGCGGGGGCCCGGCGCGGCCTACCACATGTTCGTGGTGATGGAGGACTT
  GGTGGAGAAGCTGAAGCTGCTCCGCTACGAGGAGGAGTTCCTCCGGAAGAGCAACCTGAAGGCCCCGTCC
  AGACACTATTTTGCACTGCCTACCAACCCTGGCGAACAGTTCTACATGTTTTGTACTCTTGCTGCTTGGT
  TGATTAATAAAGCGGGACGTCCCTTTGAGCAGCCTCAAGAATATGATGACCCTAATGCAACAATATCTAA
  CATACTATCCGAGCTTCGGTCATTTGGAAGAACTGCAGATTTTCCTCCTTCAAAATTAAAGTCAGGTTAT
  GGAGAACATGTATGCTATGTTCTTGATTGCTTCGCTGAAGAAGCATTGAAATATATTGGTTTCACCTGGA
  AAAGGCCAATATACCCAGTAGAAGAATTAGAAGAAGAAAGCGTTGCAGAAGATGATGCAGAATTAACATT
  AAATAAAGTGGATGAAGAATTTGTGGAAGAAGAGACAGATAATGAAGAAAACTTTATTGATCTCAACGTT
  TTAAAGGCCCAGACATATCACTTGGATATGAACGAGACTGCCAAACAAGAAGATATTTTGGAATCCACAA
  CAGATGCTGCAGAATGGAGCCTAGAAGTGGAACGTGTACTACCGCAACTGAAAGTCACGATTAGGACTGA
  CAATAAGGATTGGAGAATCCATGTTGACCAAATGCACCAGCACAGAAGTGGAATTGAATCTGCTCTAAAG
  GAGACCAAGGGATTTTTGGACAAACTCCATAATGAAATTACTAGGACTTTGGAAAAGATCAGCAGCCGAG
  AAAAGTACATCAACAATCAGCTTGAGAATTTGGTTCAAGAATATCGTGCAGCTCAAGCCCAGCTGAGTGA
  GGCAAAGGAGCGATACCAGCAGGGAAATGGAGGAGTGACGGAAAGAACCAGACTCCTCTCTGAGGTTATG
  GAAGAATTAGAAAAGGTAAAACAAGAAATGGAAGAAAAGGGCAGCAGCATGACTGATGGTGCTCCTTTGG
  TGAAGATTAAACAGAGCTTAACAAAACTGAAGCAAGAAACTGTAGAGATGGACATTAGAATTGGCATTGT
  GGAACACACACTACTCCAATCAAAGCTGAAGGAGAAGTCCAACATGACTAGGAACATGCATGCCACAGTT
  ATTCCAGAACCAGCAACAGGCTTTTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204116 representing NM_018010
  Red=Cloning site Green=Tags(s)
  
  MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPS
  RHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGY
  GEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNV
  LKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALK
  ETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVM
  EELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATV
  IPEPATGFY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018010
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018010.4
• RefSeq Size: 3052 bp
• RefSeq ORF: 1290 bp
• Locus ID: 55081
• UniProt ID: Q9NWB7
• Cytogenetics: 3q13.12-q13.13
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.[UniProtKB/Swiss-Prot Function]