ILVBL (NM_006844) Human Tagged ORF Clone

CAT#: RG203987

ILVBL (tGFP-tagged) - Human ilvB (bacterial acetolactate synthase)-like (ILVBL)

Product Images

Circular map for RG203987

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ILVBL
• Synonyms: 209L8; AHAS; HACL1L; ILV2H
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203987 representing NM_006844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCCCGCGGCCGCCGCCCCCGCTGGGAGCTTATTCCCCTCCTTCCTGCTCCTGGCCTGCGGGA
  CGCTGGTGGCCGCCTTGCTGGGCGCCGCTCACCGCCTGGGGCTCTTCTATCAGCTGCTGCACAAGGTGGA
  CAAGGCAAGCGTCCGGCATGGCGGAGAGAACGTGGCCGCTGTGCTGAGGGCCCATGGTGTGCGGTTCATC
  TTCACGCTGGTCGGTGGGCACATTTCCCCGCTGCTGGTGGCCTGTGAGAAACTGGGCATCCGTGTGGTGG
  ACACACGCCATGAGGTCACGGCCGTCTTTGCTGCTGATGCTATGGCCCGCCTGTCCGGGACGGTGGGCGT
  GGCGGCAGTGACAGCAGGCCCTGGCCTCACCAACACGGTGACTGCGGTGAAGAATGCTCAGATGGCTCAG
  TCCCCAATCCTGCTTCTGGGTGGGGCTGCCAGCACTCTGCTGCAGAACCGGGGTGCGCTCCAGGCTGTTG
  ATCAGCTGTCCCTTTTCCGGCCACTCTGTAAGTTTTGTGTGTCTGTGCGGAGGGTGCGGGACATTGTGCC
  CACCCTGAGGGCCGCGATGGCTGCCGCCCAGTCGGGCACCCCAGGTCCGGTGTTTGTGGAGCTGCCCGTT
  GACGTGCTGTACCCCTACTTCATGGTCCAGAAGGAGATGGTGCCAGCCAAGCCACCCAAGGGCCTCGTGG
  GCCGAGTGGTCTCCTGGTATTTAGAGAATTACCTGGCCAACCTCTTTGCAGGAGCCTGGGAGCCTCAGCC
  CGAGGGACCGCTGCCCCTGGACATCCCCCAGGCTTCCCCGCAGCAGGTTCAGCGCTGTGTGGAGATCCTG
  AGCCGGGCCAAGAGGCCTCTGATGGTGCTGGGGAGTCAGGCCCTGCTCACCCCAACGTCTGCCGACAAGC
  TTCGGGCTGCCGTGGAGACCTTGGGTGTTCCCTGCTTCCTTGGAGGGATGGCACGGGGGCTGTTAGGCCG
  CAACCACCCCCTCCACATCCGGGAGAACCGCAGTGCGGCCCTGAAGAAGGCGGACGTCATTGTCCTAGCA
  GGAACTGTGTGTGACTTCCGCCTATCCTATGGCCGTGTCCTCAGCCACAGCAGCAAGATCATCATCGTCA
  ATCGTAATCGGGAAGAGATGTTGCTCAACTCAGACATCTTCTGGAAGCCCCAGGAGGCTGTGCAGGGAGA
  TGTGGGTTCCTTCGTGCTGAAGTTAGTGGAGGGCCTTCAGGGCCAGACCTGGGCCCCAGACTGGGTGGAG
  GAGCTGCGGGAAGCCGACCGGCAGAAGGAGCAGACCTTTCGGGAGAAGGCAGCGATGCCTGTGGCCCAGC
  ACCTGAACCCAGTGCAGGTGCTGCAGCTGGTGGAGGAAACGCTACCTGACAACTCAATTCTGGTGGTGGA
  TGGCGGGGACTTCGTGGGCACTGCTGCCCATCTGGTACAGCCCCGCGGCCCCCTGCGCTGGCTCGATCCT
  GGGGCCTTTGGGACTCTGGGAGTTGGTGCAGGATTTGCACTTGGGGCCAAGCTGTGCCGGCCAGATGCTG
  AGGTCTGGTGCCTGTTTGGGGACGGAGCTTTTGGCTACAGCCTCATCGAATTTGATACATTCGTCAGACA
  CAAGATCCCAGTGATGGCCTTGGTAGGGAATGATGCTGGCTGGACACAGATTTCTCGGGAGCAGGTGCCC
  TCTCTGGGCAGCAACGTGGCCTGTGGCCTGGCCTACACTGATTATCACAAGGCAGCCATGGGTCTGGGGG
  CCCGGGGCTTGCTGCTCTCACGGGAGAACGAGGATCAGGTGGTCAAGGTGCTGCACGATGCCCAGCAGCA
  GTGCCGAGACGGCCACCCGGTTGTGGTCAACATCCTCATTGGGAGGACGGACTTCCGCGATGGCTCCATT
  GCTGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203987 representing NM_006844
  Red=Cloning site Green=Tags(s)
  
  METPAAAAPAGSLFPSFLLLACGTLVAALLGAAHRLGLFYQLLHKVDKASVRHGGENVAAVLRAHGVRFI
  FTLVGGHISPLLVACEKLGIRVVDTRHEVTAVFAADAMARLSGTVGVAAVTAGPGLTNTVTAVKNAQMAQ
  SPILLLGGAASTLLQNRGALQAVDQLSLFRPLCKFCVSVRRVRDIVPTLRAAMAAAQSGTPGPVFVELPV
  DVLYPYFMVQKEMVPAKPPKGLVGRVVSWYLENYLANLFAGAWEPQPEGPLPLDIPQASPQQVQRCVEIL
  SRAKRPLMVLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNHPLHIRENRSAALKKADVIVLA
  GTVCDFRLSYGRVLSHSSKIIIVNRNREEMLLNSDIFWKPQEAVQGDVGSFVLKLVEGLQGQTWAPDWVE
  ELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVEETLPDNSILVVDGGDFVGTAAHLVQPRGPLRWLDP
  GAFGTLGVGAGFALGAKLCRPDAEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVP
  SLGSNVACGLAYTDYHKAAMGLGARGLLLSRENEDQVVKVLHDAQQQCRDGHPVVVNILIGRTDFRDGSI
  AV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006844
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006844.2
• RefSeq Size: 2328 bp
• RefSeq ORF: 1899 bp
• Locus ID: 10994
• UniProt ID: A1L0T0
• Cytogenetics: 19p13.12
• Domains: TPP_enzymes
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq, Jul 2008]