CCDC74B (NM_207310) Human Tagged ORF Clone

CAT#: RG203866

CCDC74B (tGFP-tagged) - Human coiled-coil domain containing 74B (CCDC74B)

Product Images

Circular map for RG203866

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC74B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203866 representing NM_207310
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGTGCGGGGGTGGCGGCTGGGACGCGGCCCCCCAGCTCGCCGACCCCGGGCTCTCGGCGCCGGC
  GCCAGCGCCCCTCTGTGGGCGTCCAGTCCTTGAGGCCGCAGAGCCCGCAGCTCAGGCAGAGCGACCCGCA
  GAAACGGAACCTGGACCTGGAGAAGAGCCTACAGTTCCTGCAGCAGCAGCACTCGGAGATGCTGGCCAAG
  CTCCATGAGGAGATCGAGCATCTGAAGCGGGAAAACAAGGATCTCCGTTACAAGCTCATAATGAATCAGA
  CATCACAGAAGAAAGATGGCCCCTCAGGAAACCACCTTTCCAGGGCCTCTGCTCCCTTGGGCGCTCGCTG
  GGTCTGCATCAACGGAGTGTGGGTAGAGCCGGGAGGACCCAGCCCTGCCAGGCTGAAGGAGGGCTCCTCA
  CGGACACACAGGCCAGGAGGCAAGCATGGGCGTCTTGCGGGCGGTAGCGCCGACACTGTGCGCTCTCCTG
  CAGACAGCCTCTCCACGTCAAGCTTCCAGTCTGTCAAGTCCATCTCTAATTCAGGCAAGGCCAGGCCCCA
  GCCCGGCTCCTTCAACAAGCAAGATTCAAAAGCTGACGTCCCCCAGAAGGCGGACCTGGAAGAGGAGCCC
  CTACTTCACAACAGCAAGCTGGACAAGGTTCCTGGGGTACAAGGGCAGGCCAGAAAGGAGAAAGCAGAGG
  CCTCTAACGCAGGAGCTGCCTGTATGGGGAACAGCCAGCACCAGGGCAGGCAGATGGGGGCGGCGGCACA
  CCCCCCAATGATCCTGCCCCTTCCCCTGCGAAAGCCCACCACACTTAGGCAGTGCGAAGTGCTCATCCGC
  GAGCTGTGGAATACCAACCTCCTGCAGACCCAAGAGCTGCAGCACCTCAAGTCCCTCCTGGAAGGGAGCC
  AGAGGCCCCAGGCAGTCCCGGAGGAAGCTAGCTTTCCCAGGGACCAAGAAGCCACGCATTTCCCCAAGGT
  CTCCACCAAGAGCCTCTCCAAGAAATGCCTGCTTCTGAGCCCACCTGTGGCGGAGCGTGCCATCCTGCCC
  GCACTGAAGCAGACCCCGAAGAACAACTTTGCCGAGAGGCAGAAGAGGCTGCAGGCAATGCAGAAACGGC
  GCCTGCATCGCTCGGTGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203866 representing NM_207310
  Red=Cloning site Green=Tags(s)
  
  MSGAGVAAGTRPPSSPTPGSRRRRQRPSVGVQSLRPQSPQLRQSDPQKRNLDLEKSLQFLQQQHSEMLAK
  LHEEIEHLKRENKDLRYKLIMNQTSQKKDGPSGNHLSRASAPLGARWVCINGVWVEPGGPSPARLKEGSS
  RTHRPGGKHGRLAGGSADTVRSPADSLSTSSFQSVKSISNSGKARPQPGSFNKQDSKADVPQKADLEEEP
  LLHNSKLDKVPGVQGQARKEKAEASNAGAACMGNSQHQGRQMGAAAHPPMILPLPLRKPTTLRQCEVLIR
  ELWNTNLLQTQELQHLKSLLEGSQRPQAVPEEASFPRDQEATHFPKVSTKSLSKKCLLLSPPVAERAILP
  ALKQTPKNNFAERQKRLQAMQKRRLHRSVL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207310
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207310.4
• RefSeq Size: 1553 bp
• RefSeq ORF: 1143 bp
• Locus ID: 91409
• UniProt ID: Q96LY2
• Cytogenetics: 2q21.1