CCR4 NOT transcription complex subunit 3 (CNOT3) (NM_014516) Human Tagged ORF Clone

CAT#: RG203795

CNOT3 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 3 (CNOT3)

Product Images

Circular map for RG203795

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCR4 NOT transcription complex subunit 3
• Synonyms: IDDSADF; LENG2; NOT3; NOT3H
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203795 representing NM_014516
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACAAGCGCAAACTCCAAGGTGAGATTGATCGCTGCCTCAAGAAGGTGTCCGAGGGCGTGGAGC
  AGTTTGAAGATATTTGGCAGAAGCTCCACAATGCAGCCAACGCGAACCAGAAAGAAAAGTATGAGGCTGA
  CCTAAAGAAGGAGATTAAGAAGCTACAACGGCTGAGGGACCAAATCAAGACATGGGTAGCGTCCAACGAG
  ATCAAGGACAAGAGGCAGCTTATAGACAACCGCAAGCTCATTGAGACGCAAATGGAACGGTTCAAAGTTG
  TGGAACGAGAGACCAAAACCAAAGCTTACAGCAAAGAGGGCCTGGGCCTGGCCCAGAAGGTAGATCCTGC
  CCAGAAGGAGAAGGAAGAGGTTGGCCAGTGGCTCACGAATACCATCGACACGCTCAACATGCAGGTGGAC
  CAGTTTGAGAGTGAAGTGGAGTCACTGTCAGTGCAGACACGCAAGAAGAAGGGCGACAAGGATAAGCAGG
  ACCGGATTGAGGGCTTGAAGCGGCACATCGAGAAGCACCGCTACCACGTGCGCATGCTAGAGACCATCCT
  GCGCATGCTGGACAATGACTCCATCCTCGTTGACGCCATCCGCAAGATCAAGGACGACGTTGAGTACTAT
  GTTGACTCATCCCAGGACCCCGACTTCGAGGAGAACGAGTTTCTCTACGATGACCTGGACCTCGAGGACA
  TTCCACAGGCGCTGGTCGCCACCTCCCCCCCCAGCCACAGCCACATGGAGGATGAGATCTTCAACCAGTC
  CAGCAGCACGCCCACCTCAACCACCTCCAGCTCTCCCATCCCGCCCAGCCCAGCCAACTGTACCACGGAA
  AACTCTGAAGATGATAAGAAGAGGGGACGTTCCACAGACAGTGAAGTCAGCCAGTCTCCAGCCAAAAACG
  GCTCCAAGCCTGTCCACAGCAACCAGCACCCTCAGTCCCCAGCTGTGCCGCCCACCTACCCCTCCGGCCC
  CCCGCCTGCTGCCTCTGCCTTGAGCACCACTCCTGGCAACAATGGGGTCCCCGCCCCCGCAGCACCCCCA
  AGTGCCCTGGGCCCCAAGGCCAGTCCAGCTCCCAGCCACAACTCGGGCACCCCTGCTCCCTATGCCCAGG
  CGGTGGCCCCACCAGCTCCCAGTGGGCCCAGCACGACCCAGCCCCGGCCCCCCAGCGTCCAGCCTAGCGG
  AGGCGGAGGCGGCGGCAGCGGAGGCGGAGGGAGCAGCAGCAGTAGTAACAGCAGTGCCGGTGGAGGGGCT
  GGCAAGCAGAATGGCGCCACCAGTTACAGCTCAGTTGTGGCAGACAGCCCGGCAGAGGTGGCTTTGAGCA
  GCAGTGGGGGCAACAATGCCAGCAGCCAGGCCTTGGGCCCCCCTTCCGGCCCCCACAACCCACCTCCCAG
  CACCTCGAAGGAACCCAGTGCGGCAGCCCCAACGGGGGCTGGGGGCGTGGCCCCAGGCTCAGGGAACAAC
  TCAGGGGGACCCAGCCTCCTGGTGCCACTGCCTGTGAATCCTCCCAGCTCCCCAACGCCCAGCTTCAGTG
  ATGCCAAGGCAGCCGGTGCCCTGCTCAATGGGCCTCCACAGTTCAGCACCGCCCCAGAAATCAAGGCCCC
  TGAGCCTCTGAGCTCCTTGAAGTCCATGGCGGAACGGGCAGCCATCAGCTCTGGCATTGAGGACCCTGTG
  CCAACGCTGCACCTGACCGAGCGAGACATCATCCTGAGCAGTACATCAGCACCTCCGGCCTCAGCCCAGC
  CGCCCCTGCAGCTGTCAGAGGTGAACATACCGCTGTCGCTGGGTGTCTGTCCACTGGGCCCTGTGCCCCT
  CACCAAGGAGCAGCTCTATCAGCAGGCCATGGAAGAGGCCGCCTGGCACCACATGCCTCACCCCTCTGAC
  TCTGAGCGTATTCGGCAGTACCTCCCCCGGAACCCCTGTCCGACGCCCCCCTACCACCACCAGATGCCAC
  CCCCACACTCGGACACTGTGGAATTCTACCAGCGCCTGTCGACCGAGACACTCTTCTTCATCTTCTACTA
  TCTGGAGGGCACTAAGGCACAGTATCTGGCAGCCAAGGCCCTAAAGAAGCAGTCATGGCGATTCCACACC
  AAGTACATGATGTGGTTCCAGAGGCACGAGGAGCCCAAGACCATCACTGACGAGTTTGAGCAGGGCACCT
  ACATCTACTTTGACTACGAGAAGTGGGGCCAGCGGAAGAAGGAAGGCTTCACCTTTGAGTACCGCTACCT
  GGAGGACCGGGACCTCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203795 representing NM_014516
  Red=Cloning site Green=Tags(s)
  
  MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNE
  IKDKRQLIDNRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVD
  QFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYY
  VDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTE
  NSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPAASALSTTPGNNGVPAPAAPP
  SALGPKASPAPSHNSGTPAPYAQAVAPPAPSGPSTTQPRPPSVQPSGGGGGGSGGGGSSSSSNSSAGGGA
  GKQNGATSYSSVVADSPAEVALSSSGGNNASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNN
  SGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPV
  PTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSD
  SERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHT
  KYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014516
• ORF Size: 2259 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014516.2, NP_055331.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 2262 bp
• Locus ID: 4849
• UniProt ID: O75175
• Cytogenetics: 19q13.42
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function]