HSPC302 (TBCK) (NM_033115) Human Tagged ORF Clone

CAT#: RG203605

TBCK (tGFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 4

Product Images

Circular map for RG203605

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HSPC302
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203605 representing NM_033115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTT
  GTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAAC
  CATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTG
  GCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGGTATCCCTCGTACT
  TGGCCCCTGAGGTAATTGCACAGGGAATTTTCAAAACCACTGATCACATGCCAAGTAAAAAACCATTGCC
  TTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTGAGCTTTGTGTGGGAAGAAAATTA
  TTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTTAA
  TAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTTTT
  GAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTATTC
  AGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATGTG
  CTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAAAG
  ATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACAAG
  GAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTTTG
  GACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGAAA
  TAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTCAT
  TCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGAGT
  ACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATCTG
  GAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTGAG
  GGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGAAG
  TGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGGCG
  TGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTGCT
  CCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAAAT
  ACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAGAT
  GATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATGCC
  ATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATACCT
  TACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGGCT
  TTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCTGT
  GTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACCTC
  CAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGATCC
  TCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGATT
  TCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAAAA
  CAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCACAT
  TTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTTAC
  ACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGCTG
  AGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAAAT
  AAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203605 representing NM_033115
  Red=Cloning site Green=Tags(s)
  
  MFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVVV
  AEHCERSLEDLLRERKPVRYPSYLAPEVIAQGIFKTTDHMPSKKPLPSGPKSDVWSLGIILFELCVGRKL
  FQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKELPETVIDLLNKCLTFHPSKRPTPDELMKDKVF
  SEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAERSIEEVYYLWCLAGGDLEKELVNK
  EIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVGGEAFYPLLEDDQSNLPH
  SNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAYPYKKNQIWKEARVDIPPLMRGLTWAALLGVE
  GAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPDLVYWQGLDSLCA
  PFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYA
  IPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERC
  VRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPDPPKTDLSRESIPLNDLKSEVSPRI
  SAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNSEDFIRGHISGSINIPFSAAFTAEGELTQGPY
  TAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICILDGGINKIKPTGLLTIPSPQI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033115
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033115.4, NP_149106.2
• RefSeq Size: 3325 bp
• RefSeq ORF: 2493 bp
• Locus ID: 93627
• UniProt ID: Q8TEA7
• Cytogenetics: 4q24
• Domains: TBC, pkinase, RHOD, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]