Hsp75 (TRAP1) (NM_016292) Human Tagged ORF Clone

CAT#: RG203439

TRAP1 (tGFP-tagged) - Human TNF receptor-associated protein 1 (TRAP1)

Product Images

Circular map for RG203439

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hsp75
• Synonyms: HSP 75; HSP75; HSP90L; TRAP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203439 representing NM_016292
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGCGAGCTGCGGGCGCTGCTGCTGTGGGGCCGCCGCCTGCGGCCTTTGCTGCGGGCGCCGGCGC
  TGGCGGCCGTGCCGGGAGGAAAACCAATTCTGTGTCCTCGGAGGACCACAGCCCAGTTGGGCCCCAGGCG
  AAACCCAGCCTGGAGCTTGCAGGCAGGACGACTGTTCAGCACGCAGACCGCCGAGGACAAGGAGGAACCC
  CTGCACTCGATTATCAGCAGCACAGAGAGCGTGCAGGGTTCCACTTCCAAACATGAGTTCCAGGCCGAGA
  CAAAGAAGCTTTTGGACATTGTTGCCCGGTCCCTGTACTCAGAAAAAGAGGTGTTTATACGGGAGCTGAT
  CTCCAATGCCAGCGATGCCTTGGAAAAACTGCGTCACAAACTGGTGTCTGACGGCCAAGCACTGCCAGAA
  ATGGAGATTCACTTGCAGACCAATGCCGAGAAAGGCACCATCACCATCCAGGATACTGGTATCGGGATGA
  CACAGGAAGAGCTGGTGTCCAACCTGGGGACGATTGCCAGATCGGGGTCAAAGGCCTTCCTGGATGCTCT
  GCAGAACCAGGCTGAGGCCAGCAGCAAGATCATCGGCCAGTTTGGAGTGGGTTTCTACTCAGCTTTCATG
  GTGGCTGACAGAGTGGAGGTCTATTCCCGCTCGGCAGCCCCGGGGAGCCTGGGTTACCAGTGGCTTTCAG
  ATGGTTCTGGAGTGTTTGAAATCGCCGAAGCTTCGGGAGTTAGAACCGGGACAAAAATCATCATCCACCT
  GAAATCCGACTGCAAGGAGTTTTCCAGCGAGGCCCGGGTGCGAGATGTGGTAACGAAGTACAGCAACTTC
  GTCAGCTTCCCCTTGTACTTGAATGGAAGGCGGATGAACACCTTGCAGGCCATCTGGATGATGGACCCCA
  AGGATGTCCGTGAGTGGCAACATGAGGAGTTCTACCGCTACGTCGCGCAGGCTCACGACAAGCCCCGCTA
  CACCCTGCACTATAAGACGGACGCACCGCTCAACATCCGCAGCATCTTCTACGTGCCCGACATGAAACCG
  TCCATGTTTGATGTGAGCCGGGAGCTGGGCTCCAGCGTTGCACTGTACAGCCGCAAAGTCCTCATCCAGA
  CCAAGGCCACGGACATCCTGCCCAAGTGGCTGCGCTTCATCCGAGGTGTGGTGGACAGTGAGGACATTCC
  CCTGAACCTCAGCCGGGAGCTGCTGCAGGAGAGCGCACTCATCAGGAAACTCCGGGACGTTTTACAGCAG
  AGGCTGATCAAATTCTTCATTGACCAGAGTAAAAAAGATGCTGAGAAGTATGCAAAGTTTTTTGAAGATT
  ACGGCCTGTTCATGCGGGAGGGCATTGTGACCGCCACCGAGCAGGAGGTCAAGGAGGACATAGCAAAGCT
  GCTGCGCTACGAGTCCTCGGCGCTGCCCTCCGGGCAGCTAACCAGCCTCTCAGAATACGCCAGCCGCATG
  CGGGCCGGCACCCGCAACATCTACTACCTGTGCGCCCCCAACCGTCACCTGGCAGAGCACTCACCCTACT
  ATGAGGCCATGAAGAAGAAAGACACAGAGGTTCTCTTCTGCTTTGAGCAGTTTGATGAGCTCACCCTGCT
  GCACCTTCGTGAGTTTGACAAGAAGAAGCTGATCTCTGTGGAGACGGACATAGTCGTGGATCACTACAAG
  GAGGAGAAGTTTGAGGACAGGTCCCCAGCCGCCGAGTGCCTATCAGAGAAGGAGACGGAGGAGCTCATGG
  CCTGGATGAGAAATGTGCTGGGGTCGCGTGTCACCAACGTGAAGGTGACCCTCCGACTGGACACCCACCC
  TGCCATGGTCACCGTGCTGGAGATGGGGGCTGCCCGCCACTTCCTGCGCATGCAGCAGCTGGCCAAGACC
  CAGGAGGAGCGCGCACAGCTCCTGCAGCCCACGCTGGAGATCAACCCCAGGCACGCGCTCATCAAGAAGC
  TGAATCAGCTGCGCGCAAGCGAGCCTGGCCTGGCTCAGCTGCTGGTGGATCAGATATACGAGAACGCCAT
  GATTGCTGCTGGACTTGTTGACGACCCTAGGGCCATGGTGGGCCGCTTGAATGAGCTGCTTGTCAAGGCC
  CTGGAGCGACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203439 representing NM_016292
  Red=Cloning site Green=Tags(s)
  
  MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEP
  LHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPE
  MEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFM
  VADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNF
  VSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKP
  SMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQ
  RLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRM
  RAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYK
  EEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKT
  QEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKA
  LERH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016292
• ORF Size: 2112 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016292.3
• RefSeq Size: 2225 bp
• RefSeq ORF: 2115 bp
• Locus ID: 10131
• UniProt ID: Q12931
• Cytogenetics: 16p13.3
• Domains: HSP90, HATPase_c
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]