RBMS1 (NM_016836) Human Tagged ORF Clone

CAT#: RG203437

RBMS1 (tGFP-tagged) - Human RNA binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 1

Product Images

Circular map for RG203437

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBMS1
• Synonyms: C2orf12; HCC-4; MSSP; MSSP-1; MSSP-2; MSSP-3; SCR2; YC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203437 representing NM_016836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAAGTGTGGAAACAGCAGATGTACCCTCAGTACGCCACCTACTATTACCCCCAGTATCTGCAAG
  CCAAGCAGTCTCTGGTCCCAGCCCACCCCATGGCCCCTCCCAGTCCCAGCACCACCAGCAGTAATAACAA
  CAGTAGCAGCAGTAGCAACTCAGGATGGGATCAGCTCAGCAAAACGAACCTCTATATCCGAGGACTGCCT
  CCCCACACCACCGACCAGGACCTGGTGAAGCTCTGTCAACCATATGGGAAAATAGTCTCCACAAAGGCAA
  TTTTGGATAAGACAACGAACAAATGCAAAGGTTATGGTTTTGTCGACTTTGACAGCCCTGCAGCAGCTCA
  AAAAGCTGTGTCTGCCCTGAAGGCCAGTGGGGTTCAAGCTCAAATGGCAAAGCAACAGGAACAAGATCCT
  ACCAACCTCTACATTTCTAATTTGCCACTCTCCATGGATGAGCAAGAACTAGAAAATATGCTCAAACCAT
  TTGGACAAGTTATTTCTACAAGGATACTACGTGATTCCAGTGGTACAAGTCGTGGTGTTGGCTTTGCTAG
  GATGGAATCAACAGAAAAATGTGAAGCTGTTATTGGTCATTTTAATGGAAAATTTATTAAGACACCACCA
  GGAGTTTCTGCCCCCACAGAACCTTTATTGTGTAAGTTTGCTGATGGAGGACAGAAAAAGAGACAGAACC
  CAAACAAATACATCCCTAATGGAAGACCATGGCATAGAGAAGGAGAGGTGAGACTTGCTGGAATGACACT
  TACTTACGACCCAACTACAGCTGCTATACAGAACGGATTTTATCCTTCACCATACAGTATTGCTACAAAC
  CGAATGATCACTCAAACTTCTATTACACCCTATATTGCATCTCCTGTATCTGCCTACCAGGTGCAAAGTC
  CTTCGTGGATGCAACCTCAACCATATATTCTACAGCACCCTGGTGCCGTGTTAACTCCCTCAATGGAGCA
  CACCATGTCACTACAGCCCGCATCAATGATCAGCCCTCTGGCCCAGCAGATGAGTCATCTGTCACTAGGC
  AGCACCGGAACATACATGCCTGCAACGTCAGCTATGCAAGGAGCCTACTTGCCACAGTATGCACATATGC
  AGACGACAGCGGTTCCTGTTGAGGAGGCAAGTGGTCAACAGCAGGTGGCTGTCGAGACGTCTAATGACCA
  TTCTCCATATACCTTTCAACCTAATAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203437 representing NM_016836
  Red=Cloning site Green=Tags(s)
  
  MGKVWKQQMYPQYATYYYPQYLQAKQSLVPAHPMAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLP
  PHTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDP
  TNLYISNLPLSMDEQELENMLKPFGQVISTRILRDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPP
  GVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRPWHREGEVRLAGMTLTYDPTTAAIQNGFYPSPYSIATN
  RMITQTSITPYIASPVSAYQVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLG
  STGTYMPATSAMQGAYLPQYAHMQTTAVPVEEASGQQQVAVETSNDHSPYTFQPNK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016836
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016836.4
• RefSeq Size: 2438 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5937
• UniProt ID: P29558
• Cytogenetics: 2q24.2
• Gene Summary: This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. Several transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. A pseudogene for this locus is found on chromosome 12. [provided by RefSeq, Feb 2009]