XLF (NHEJ1) (NM_024782) Human Tagged ORF Clone

CAT#: RG203393

NHEJ1 (tGFP-tagged) - Human nonhomologous end-joining factor 1 (NHEJ1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NHEJ1
• Synonyms: XLF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203393 representing NM_024782
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAACTCCCTCT
  TGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCTTCAACAGGTGTGGCATGA
  ACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGGCTCACTGCTCCTCCTGCA
  GCTTTCCTCTGTCATTTGGATAATCTCCTTCGCCCATTGTTGAAGGACGCTGCTCACCCTAGCGAAGCTA
  CCTTCTCCTGTGATTGTGTGGCAGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTT
  CTATTGGAATTTCCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATG
  GGCATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACTTCATATGAAAGACCTAGAGA
  TCCAAGACTACCAGGAGAGTGGGGCTACGCTGATTCGAGATCGATTGAAGACAGAACCATTTGAAGAAAA
  TTCCTTCTTGGAACAATTTATGATAGAGAAACTGCCAGAGGCATGCAGCATTGGTGATGGAAAGCCCTTT
  GTCATGAATCTGCAGGATCTGTATATGGCAGTCACCACACAAGAGGTCCAAGTGGGACAGAAGCATCAAG
  GCGCTGGAGATCCTCATACCTCAAACAGTGCTTCCCTGCAAGGAATCGATAGCCAATGTGTAAACCAGCC
  AGAACAACTGGTCTCCTCAGCCCCAACCCTCTCAGCACCTGAGAAAGAGTCCACGGGTACTTCAGGCCCT
  CTGCAGAGACCTCAGCTGTCAAAGGTCAAGAGGAAGAAGCCAAGGGGTCTCTTCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203393 representing NM_024782
  Red=Cloning site Green=Tags(s)
  
  MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPA
  AFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLM
  GMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPF
  VMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGP
  LQRPQLSKVKRKKPRGLFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024782
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024782.3
• RefSeq Size: 2037 bp
• RefSeq ORF: 900 bp
• Locus ID: 79840
• UniProt ID: Q9H9Q4
• Cytogenetics: 2q35
• Protein Pathways: Non-homologous end-joining
• Gene Summary: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]