WBSCR16 (RCC1L) (NM_030798) Human Tagged ORF Clone

CAT#: RG203234

WBSCR16 (tGFP-tagged) - Human Williams-Beuren syndrome chromosome region 16 (WBSCR16)

Product Images

Circular map for RG203234

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: WBSCR16
• Synonyms: WBSCR16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203234 representing NM_030798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGGTGGCGTTGGTGGCTGGGGCTCGGCTGGGGCGGCGGCTGAGCGGGCCGGGGCTGGGGCGAG
  GGCACTGGACGGCGGCCGGGCGCTCCCGGAGCCGGCGCGAAGCGGCAGAAGCCGAGGCGGAGGTGCCCGT
  GGTCCAGTACGTGGGCGAGCGCGCTGCCCGCGCCGATCGCGTCTTCGTGTGGGGCTTCAGCTTCTCGGGG
  GCGCTGGGCGTGCCTTCCTTTGTGGTGCCCAGCTCCGGGCCCGGGCCCCGCGCCGGCGCCCGACCGCGCC
  GCAGGATCCAGCCCGTGCCCTATCGCCTGGAGCTGGACCAAAAGATTTCATCTGCTGCTTGCGGCTATGG
  ATTCACACTGCTGTCCTCTAAGACTGCGGATGTTACGAAAGTCTGGGGGATGGGACTCAACAAAGATTCT
  CAGCTTGGATTTCACAGGAGCCGGAAAGATAAAACGAGGGGCTACGAGTATGTGTTGGAGCCCTCACCCG
  TCTCCCTGCCTCTGGACAGACCTCAGGAGACACGGGTGCTGCAGGTCTCCTGCGGCCGAGCTCACTCTCT
  TGTGTTGACTGACAGGGAAGGAGTCTTCAGCATGGGAAACAATTCTTATGGGCAATGTGGAAGAAAGGTG
  GTCGAAAATGAAATTTACAGTGAAAGTCACAGAGTCCACAGGATGCAGGACTTCGATGGCCAGGTGGTCC
  AGGTCGCCTGTGGTCAGGATCATAGTCTGTTCCTGACGGATAAAGGAGAAGTCTATTCTTGTGGATGGGG
  TGCTGATGGGCAAACAGGTCTGGGTCACTACAATATCACCAGCTCGCCCACCAAGCTGGGTGGAGACCTG
  GCGGGAGTGAACGTTATCCAAGTTGCCACCTACGGTGATTGCTGCCTGGCCGTGTCCGCCGACGGAGGAC
  TTTTTGGTTGGGGAAACTCGGAGTACCTGCAGCTGGCCTCTGTCACTGACTCCACACAGGTGAATGTGCC
  CCGCTGCTTACACTTCTCAGGAGTGGGGAAGGTGCGACAGGCTGCATGCGGTGGCACGGGCTGTGCAGTG
  TTAAACGGAGAAGGACATGTTTTTGTCTGGGGCTATGGAATTCTTGGGAAAGGTCCAAACCTAGTGGAAA
  GTGCCGTCCCTGAAATGATTCCACCCACTCTCTTTGGCTTGACGGAGTTCAACCCAGAAATCCAGGTTTC
  CCGCATCCGATGTGGACTCAGCCACTTTGCTGCACTGACCAACAAAGGAGAGCTGTTTGTATGGGGCAAG
  AACATCCGAGGGTGCCTGGGAATCGGTCGCCTGGAGGACCAGTATTTCCCATGGAGGGTGACGATGCCTG
  GGGAGCCTGTGGACGTGGCATGTGGCGTGGACCACATGGTGACCCTGGCCAAGTCATTCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203234 representing NM_030798
  Red=Cloning site Green=Tags(s)
  
  MALVALVAGARLGRRLSGPGLGRGHWTAAGRSRSRREAAEAEAEVPVVQYVGERAARADRVFVWGFSFSG
  ALGVPSFVVPSSGPGPRAGARPRRRIQPVPYRLELDQKISSAACGYGFTLLSSKTADVTKVWGMGLNKDS
  QLGFHRSRKDKTRGYEYVLEPSPVSLPLDRPQETRVLQVSCGRAHSLVLTDREGVFSMGNNSYGQCGRKV
  VENEIYSESHRVHRMQDFDGQVVQVACGQDHSLFLTDKGEVYSCGWGADGQTGLGHYNITSSPTKLGGDL
  AGVNVIQVATYGDCCLAVSADGGLFGWGNSEYLQLASVTDSTQVNVPRCLHFSGVGKVRQAACGGTGCAV
  LNGEGHVFVWGYGILGKGPNLVESAVPEMIPPTLFGLTEFNPEIQVSRIRCGLSHFAALTNKGELFVWGK
  NIRGCLGIGRLEDQYFPWRVTMPGEPVDVACGVDHMVTLAKSFI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030798
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030798.5
• RefSeq Size: 2386 bp
• RefSeq ORF: 1395 bp
• Locus ID: 81554
• UniProt ID: Q96I51
• Cytogenetics: 7q11.23
• Domains: RCC1
• Gene Summary: This gene encodes a protein containing regulator of chromosome condensation 1-like repeats. The encoded protein may function as a guanine nucleotide exchange factor. This gene is located in a region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]