SC65 (P3H4) (NM_006455) Human Tagged ORF Clone

CAT#: RG203203

LEPREL4 (tGFP-tagged) - Human leprecan-like 4 (LEPREL4)

Product Images

Circular map for RG203203

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SC65
• Synonyms: LEPREL4; NO55; NOL55; SC65
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203203 representing NM_006455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGGTGGCGTGGGGGCTGCTGTGGTTGCTGCTGGGCAGCGCCGGGGCGCAGTACGAGAAGTACA
  GCTTCCGGGGCTTCCCGCCCGAGGACCTGATGCCGCTGGCCGCGGCGTACGGGCACGCTCTGGAGCAGTA
  CGAGGGAGAGAGCTGGCGCGAGAGCGCGCGCTACCTGGAGGCGGCGCTGCGGCTGCACCGGCTCCTGCGC
  GACAGCGAGGCCTTCTGCCACGCCAACTGCAGCGGCCCCGCGCCCGCGGCCAAGCCCGATCCCGACGGCG
  GCCGCGCAGACGAGTGGGCCTGCGAGCTGCGGCTCTTCGGCCGCGTCCTGGAGCGAGCCGCCTGCCTGCG
  GCGCTGCAAGCGGACGCTGCCCGCCTTCCAGGTGCCCTACCCGCCGCGGCAGCTGCTGCGTGACTTCCAG
  AGCCGCCTGCCCTACCAGTACCTGCACTACGCGCTGTTCAAGGCTAACCGGCTGGAGAAGGCGGTGGCGG
  CGGCCTACACCTTCCTCCAGAGGAACCCGAAGCACGAGCTGACCGCCAAGTATCTCAACTACTATCAGGG
  GATGCTGGACGTCGCCGACGAGTCCCTCACGGACCTAGAGGCCCAGCCCTACGAGGCCGTGTTCCTCCGG
  GCTGTGAAGCTCTACAACAGCGGGGATTTCCGCAGCAGCACGGAGGACATGGAGCGGGCCTTGTCAGAGT
  ACCTGGCAGTCTTTGCCCGGTGCCTGGCCGGCTGTGAAGGGGCCCATGAGCAGGTGGACTTCAAGGACTT
  CTACCCGGCCATAGCAGATCTCTTTGCAGAGTCCCTGCAGTGCAAGGTGGACTGTGAGGCCAATTTGACC
  CCCAATGTGGGTGGCTACTTCGTGGACAAGTTCGTGGCCACCATGTACCACTACCTGCAGTTTGCCTACT
  ATAAGTTGAATGATGTGCGCCAGGCTGCCCGCAGCGCCGCCAGCTACATGCTCTTCGACCCCAAGGACAG
  CGTCATGCAGCAGAACCTGGTGTATTACCGGTTCCACCGGGCTCGCTGGGGCCTGGAAGAGGAGGACTTC
  CAGCCCCGGGAGGAGGCCATGCTCTACCACAACCAGACCGCCGAGCTGCGGGAGCTGCTGGAGTTCACCC
  ACATGTACCTGCAGTCAGATGATGAGATGGAGCTGGAGGAGACAGAACCGCCCCTGGAGCCTGAGGATGC
  CCTATCTGACGCCGAGTTTGAGGGGGAGGGTGACTACGAGGAGGGCATGTATGCTGACTGGTGGCAGGAG
  CCGGATGCCAAGGGTGACGAGGCCGAGGCTGAGCCAGAGCCTGAACTCGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203203 representing NM_006455
  Red=Cloning site Green=Tags(s)
  
  MARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLR
  DSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQ
  SRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLR
  AVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLT
  PNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDF
  QPREEAMLYHNQTAELRELLEFTHMYLQSDDEMELEETEPPLEPEDALSDAEFEGEGDYEEGMYADWWQE
  PDAKGDEAEAEPEPELA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006455
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006455.3
• RefSeq Size: 2619 bp
• RefSeq ORF: 1314 bp
• Locus ID: 10609
• UniProt ID: Q92791
• Cytogenetics: 17q21.2
• Gene Summary: This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq, Jul 2008]