JSRP1 (NM_144616) Human Tagged ORF Clone

CAT#: RG203004

JSRP1 (tGFP-tagged) - Human junctional sarcoplasmic reticulum protein 1 (JSRP1)

Product Images

Circular map for RG203004

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: JSRP1
• Synonyms: JP-45; JP45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203004 representing NM_144616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATGACAACCAGAGCCTGGGAGGAGCTGGATGGCGGCCTGGGCAGCTGCCAGGCCCTGGAGGACC
  ACTCTGCGCTGGCCGAGACCCAGGAGGACAGGGCTTCAGCGACACCCAGGCTGGCCGACTCCGGCAGCGT
  GCCCCACGACTCTCAGGTGGCTGAAGGCCCCAGTGTGGACACCAGGCCCAAGAAGATGGAAAAAGAGCCT
  GCCGCCAGGGGGACCCCAGGAACGGGGAAGGAGAGGCTGAAAGCCGGAGCGAGCCCTCGGAGCGTCCCCG
  CGCGCAAGAAGGCGCAGACCGCGCCGCCCCTGCAGCCGCCGCCGCCGCCCCCGGCCCTGAGCGAGGAGCT
  GCCCTGGGGAGACCTGTCGCTCAACAAGTGCCTGGTGCTCGCCTCGCTGGTGGCGCTGCTGGGCTCGGCT
  TTCCAGCTGTGCCGCGACGCCGTCCCTGGGGAGGCAGCACTCCAAGCACGTGTGCCCGAGCCCTGGGTCC
  CGCCAAGCTCAGCCCCGAGGGAGCCATCGTCGCCCCTGCCTAAGTTCGAGGCCCAGGCGCCTCCATCAGC
  GCCGCCTGCGCCCCGGGCCGAGGCAGAGGTCAGACCCAAGATTCCCGGGAGTCGGGAGGCTGCAGAGAAC
  GACGAAGAGGAGCCCGGCGAGGCCACCGGAGAGGCCGTCCGGGAGGACCGTGTGACCCTCGCAGACCGGG
  GACCCAAGGAGAGGCCTCGGAGAGAGGGGAAGCCGCGGAAGGAGAAGCCGCGGAAGGAGGAGAGACCTAA
  GAAAGAGAGGCCGCGGAAAGAGGAGAGGCCACGGGCCGCCAGGGAGCCCCGGGAAGCCCTACCCCAGCGC
  TGGGAGTCACGCGAAGGGGGCCACCGGCCGTGGGCACGGGACTCCAGGGACGCCGAGCCCAGGAAGAAGC
  AGGCCTGGGTGTCCCCGAGGCGTCCCGACGAGGAGCAGCGGCCTGGGAGTCGCCAGAAGCTCCGCGCAGG
  CAAGGGGCGGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203004 representing NM_144616
  Red=Cloning site Green=Tags(s)
  
  MSMTTRAWEELDGGLGSCQALEDHSALAETQEDRASATPRLADSGSVPHDSQVAEGPSVDTRPKKMEKEP
  AARGTPGTGKERLKAGASPRSVPARKKAQTAPPLQPPPPPPALSEELPWGDLSLNKCLVLASLVALLGSA
  FQLCRDAVPGEAALQARVPEPWVPPSSAPREPSSPLPKFEAQAPPSAPPAPRAEAEVRPKIPGSREAAEN
  DEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEERPRAAREPREALPQR
  WESREGGHRPWARDSRDAEPRKKQAWVSPRRPDEEQRPGSRQKLRAGKGRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144616
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144616.4
• RefSeq Size: 1145 bp
• RefSeq ORF: 996 bp
• Locus ID: 126306
• UniProt ID: Q96MG2
• Cytogenetics: 19p13.3
• Gene Summary: The protein encoded by this gene is involved in excitation-contraction coupling at the sarcoplasmic reticulum. The encoded protein can interact with CACNA1S, CACNB1, and calsequestrin to help regulate calcium influx and efflux in skeletal muscle. [provided by RefSeq, Jul 2012]