MGC12965 (NM_198519) Human Tagged ORF Clone

CAT#: RG202962

MGC12965 (tGFP-tagged) - Human similar to Cytochrome c, somatic (MGC12965)

Product Images

Circular map for RG202962

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MGC12965
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202962 representing NM_198519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCATATATGACCTGAAGAAACAGAAGGATAAACTGTTGAAGTTTTATGCTGAAAGTGATGAGC
  AGATATTAATGAAAAATAGAAAAACACTTCATAAAGCTAAAAATGAAGATCTTGATCGTGTATTGAAAGA
  GTGGATCCGTCAGCGTCGCAGTGAACACATGCCACTTAATGGTATGCTGATCATGAAACAAGCAAAGATA
  TATCACAATGAACTAAAAATTGAGGGGAACTGTGAATATTCAACAGGCTGGTTGCAGAAATTTAAGAAAA
  GACATGGCATTAAATTTTTAAAGACTTGTGGCAATAAAGCATCTGCTGGTCATGAAGCAACAGAGAAGTT
  TACTGGCAAGTTCAGTAATGATGATGAACAAGATGGTAACTTTGAAGGATTCAGTATGTCAAGTGAGAAA
  AAAATAATGTCTGACCTCCTTACATATACAAAAAATATACATCCAGAGACTGTCAGTAAGCTGGAAGAAG
  AGGATATCAAAGATGTTTTTAACAGTAATAATGAGGCTCCAGTTGTTCATTCATTGTCCAATGGTGAAGT
  AACAAAAATGGTTCTGAATCAAGATGATCATGATGATAATGATAATGAAGATGATGTTAACACTGCAGAA
  AAAGTGCCTATAGACGACATGGTAAAAATGTGTGATGGGCTTATTAAAGGACTAGAGCAGCATGCATTCA
  TAACAGAGCAAGAAATCATGTCAGTTTATAAAATCAAAGAGAGACTTCTAAGACAAAAAGCATCATTAAT
  GAGGCAGATGACTCTGAAAGAAACATTTAAAAAAGCCATCCAGAGGAATGCTTCTTCCTCTCTACAGGAC
  CCACTTCTTGGTCCCTCAACTGCTTCTGATGCTTCTTCTCACCTAAAAATAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202962 representing NM_198519
  Red=Cloning site Green=Tags(s)
  
  MTTIYDLKKQKDKLLKFYAESDEQILMKNRKTLHKAKNEDLDRVLKEWIRQRRSEHMPLNGMLIMKQAKI
  YHNELKIEGNCEYSTGWLQKFKKRHGIKFLKTCGNKASAGHEATEKFTGKFSNDDEQDGNFEGFSMSSEK
  KIMSDLLTYTKNIHPETVSKLEEEDIKDVFNSNNEAPVVHSLSNGEVTKMVLNQDDHDDNDNEDDVNTAE
  KVPIDDMVKMCDGLIKGLEQHAFITEQEIMSVYKIKERLLRQKASLMRQMTLKETFKKAIQRNASSSLQD
  PLLGPSTASDASSHLKIK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198519
• ORF Size: 894 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198519.1, NP_940921.1
• RefSeq Size: 2145 bp
• RefSeq ORF: 896 bp
• Locus ID: 374408
• Cytogenetics: 11q13.4